Abstract:
BACKGROUND:Mesenchymal stromal/stem cells derived from human umbilical cord (UC-MSCs) uniquely combine properties of embryonic and postnatal MSCs and may be the most acceptable, safe, and effective source for allogeneic cell therapy e.g. for therapeutic angiogenesis. In this report we describe pro-angiogenic properties of UC-MSCs as manifested in vitro. METHODS:UC-MSCs were isolated from human Wharton's jelly by enzymatic digestion. Presence of soluble forms of VEGF-A in UC-MSC-conditioned media was measured by ELISA. Effects of the conditioned media on human umbilical vein-derived endothelial EA.hy926 cells proliferation were measured by MTT-assay; changes in cell motility and directed migration were assessed by scratch wound healing and transwell chamber migration assays. Angiogenesis was modeled in vitro as tube formation on basement membrane matrix. Progressive differentiation of MSCs to endothelioid progeny was assessed by CD31 immunostaining. RESULTS:Although no detectable quantities of soluble VEGF-A were produced by UC-MSCs, the culture medium, conditioned by the UC-MSCs, effectively stimulated proliferation, motility, and directed migration of EA.hy926 cells. In 2D culture, UC-MSCs were able to acquire CD31(+) endothelial cell-like phenotype when stimulated by EA.hy926-conditioned media supplemented with VEGF-A165. UC-MSCs were capable of forming unstable 2D tubular networks either by themselves or in combinations with EA.hy926 cells. Active spontaneous sprouting from cell clusters, resulting from disassembling of such networks, was observed only in the mixed cultures, not in pure UC-MSC cultures. In 3D mode of sprouting experimentation, structural support of newly formed capillary-like structures was provided by UC-MSCs that acquired the CD31(+) phenotype in the absence of exogenous VEGF-A. CONCLUSION:These data suggest that a VEGF-A-independent paracrine mechanism and at least partially VEGF-A-independent differentiation mechanism are involved in the pro-angiogenic activity of UC-MSCs.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Arutyunyan I,Fatkhudinov T,Kananykhina E,Usman N,Elchaninov A,Makarov A,Bolshakova G,Goldshtein D,Sukhikh Gdoi
10.1186/s13287-016-0305-4subject
Has Abstractpub_date
2016-03-22 00:00:00pages
46issn
1757-6512pii
10.1186/s13287-016-0305-4journal_volume
7pub_type
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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abstract:INTRODUCTION:New therapeutic options are necessary for patients with chronic Chagas disease, a leading cause of heart failure in Latin American countries. Stem cell therapy focused on improving cardiac function is a promising approach for treating heart disease. Here, we evaluated the therapeutic effects of cardiac mes...
journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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abstract:INTRODUCTION:Despite attempts to prevent brain injury during the hyperacute phase of stroke, most sufferers end up with significant neuronal loss and functional deficits. The use of cell-based therapies to recover the injured brain offers new hope. In the current study, we employed human neural stem cells (hNSCs) isola...
journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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abstract:INTRODUCTION:Administration of bone marrow-derived cells produces beneficial effects in experimental extrapulmonary acute respiratory distress syndrome (ARDS). However, there are controversies regarding the effects of timing of cell administration and initial insult severity on inflammatory response. We evaluated the e...
journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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更新日期:2017-01-23 00:00:00
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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abstract:INTRODUCTION:Stem cell therapy has emerged as potential therapeutic strategy for damaged heart muscles. Umbilical cord blood (UCB) cells are the most prevalent stem cell source available, yet have not been fully tested in cardiac regeneration. Herein, studies were performed to evaluate the cardiovascular safety and ben...
journal_title:Stem cell research & therapy
pub_type: 杂志文章
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journal_title:Stem cell research & therapy
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journal_title:Stem cell research & therapy
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