HisAK70: progress towards a vaccine against different forms of leishmaniosis.

Abstract:

BACKGROUND:Leishmania major and Leishmania infantum are among the main species that are responsible for cutaneous leishmaniosis (CL) and visceral leishmaniosis (VL), respectively. The leishmanioses represent the second-largest parasitic killer in the world after malaria. Recently, we succeeded in generating a plasmid DNA (pCMV-HISA70m2A) and demonstrated that immunized mice were protected against L. major challenge. The efficacy of the DNA-vaccine was further enhanced by the inclusion of KMP-11 antigen into the antibiotic-free plasmid pVAX1-asd. METHODS:Here, we describe the use of a HisAK70 DNA-vaccine encoding seven Leishmania genes (H2A, H2B, H3, H4, A2, KMP11 and HSP70) for vaccination of mice to assess the induction of a resistant phenotype against VL and CL. RESULTS:HisAK70 was successful in vaccinated mice, resulting in a high amount of efficient sterile hepatic granulomas associated with a hepatic parasite burden fully resolved in the VL model; and resulting in 100% inhibition of parasite visceralization in the CL model. CONCLUSIONS:The results suggest that immunization with the HisAK70 DNA-vaccine may provide a rapid, suitable, and efficient vaccination strategy to confer cross-protective immunity against VL and CL.

journal_name

Parasit Vectors

journal_title

Parasites & vectors

authors

Domínguez-Bernal G,Horcajo P,Orden JA,Ruiz-Santa-Quiteria JA,De La Fuente R,Ordóñez-Gutiérrez L,Martínez-Rodrigo A,Mas A,Carrión J

doi

10.1186/s13071-015-1246-y

subject

Has Abstract

pub_date

2015-12-09 00:00:00

pages

629

issn

1756-3305

pii

10.1186/s13071-015-1246-y

journal_volume

8

pub_type

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