Abstract：
INTRODUCTION:Adipose-derived stroma cells (ASCs) are attractive cells for cell-based gene therapy but are generally difficult to transfect. Nucleofection has proven to be an efficient method for transfection of primary cells. Therefore, we used this technique to transfect ASCs with a vector encoding for Ambystoma mexicanum epidermal lipoxygenase (AmbLOXe) which is a promising bioactive enzyme in regenerative processes. Thereby, we thought to even further increase the large regenerative potential of the ASCs. METHODS:ASCs were isolated from the inguinal fat pad of Lewis rats and were subsequently transfected in passage 1 using Nucleofector® 2b and the hMSC Nucleofector kit. Transfection efficiency was determined measuring co-transfected green fluorescent protein (GFP) in a flow cytometer and gene expression in transfected cells was detected by reverse transcription polymerase chain reaction (RT-PCR). Moreover, cell migration was assessed using a scratch assay and results were tested for statistical significance with ANOVA followed by Bonferroni's post hoc test. RESULTS:High initial transfection rates were achieved with an average of 79.8 ± 2.82% of GFP positive cells although longer cultivation periods reduced the number of positive cells to below 5% after four passages. Although successful production of AmbLOXe transcript could be proven the gene product had no measureable effect on cell migration. CONCLUSIONS:Our study demonstrates the feasibility of ASCs to serve as a vehicle of AmbLOXe transport for gene therapeutic purposes in regenerative medicine. One potential field of applications could be peripheral nerve injuries.
journal_name
Stem Cell Res Therjournal_title
Stem cell research & therapyauthors
Fülbier A,Schnabel R,Michael S,Vogt PM,Strauß S,Reimers K,Radtke Cdoi
10.1186/scrt503subject
Has Abstractpub_date
2014-10-09 00:00:00pages
113issue
5issn
1757-6512pii
scrt503journal_volume
5pub_type
杂志文章abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that affects female fertility. However, with the lack of a corresponding research model, the pathology mechanism of PCOS is poorly understood. Induced pluripotent stem cell (iPSC) technology has been recognized as means to generate...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0950-x
更新日期：2018-08-09 00:00:00
abstract：BACKGROUND:Dystrophic epidermolysis bullosa (DEB), a rare genodermatosis, is characterized by the formation of intra-epidermal blistering and the development of chronic nonhealing skin wounds. Recently, attempts have been made to develop cell-based therapies for this currently intractable disorder. The molecular mechan...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0388-y
更新日期：2016-08-27 00:00:00
abstract：BACKGROUND:A keloid is a fibroproliferative disorder occurring in wounds characterized by an exaggerated response to injury. To date, no effective cure has been identified. As multipotent stem cells, human adipose-derived stem cells (ADSCs) may show the possibility for curing diseases such as fibrosis. This study sough...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0786-4
更新日期：2018-02-21 00:00:00
abstract：BACKGROUND:Mesenchymal stromal cells (MSCs) are an attractive therapeutic agent in regenerative medicine. Recently, there has been a paradigm shift from differentiation of MSCs to their paracrine effects at the injury site. Several reports elucidate the role of trophic factors secreted by MSCs toward the repair of inju...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0752-6
更新日期：2018-02-06 00:00:00
abstract：AIM:Myocardial infarction (MI) is a severe disease with increased mortality and disability rates, posing heavy economic burden for society. Exosomes were uncovered to mediate intercellular communication after MI. This study aims to explore the effect and mechanism of lncRNA KLF3-AS1 in exosomes secreted by human mesenc...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1522-4
更新日期：2019-12-17 00:00:00
abstract：BACKGROUND:C-kit-positive cardiac stem cells (CSCs) have been shown to be a promising candidate treatment for myocardial infarction and heart failure. Insulin-like growth factor (IGF)-1 is an anabolic growth hormone that regulates cellular proliferation, differentiation, senescence, and death in various tissues. Althou...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-017-0762-4
更新日期：2018-02-21 00:00:00
abstract：BACKGROUND:Mesenchymal stromal/stem cells derived from human umbilical cord (UC-MSCs) uniquely combine properties of embryonic and postnatal MSCs and may be the most acceptable, safe, and effective source for allogeneic cell therapy e.g. for therapeutic angiogenesis. In this report we describe pro-angiogenic properties...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0305-4
更新日期：2016-03-22 00:00:00
abstract：BACKGROUND:Currently, the overall therapeutic efficiency of mesenchymal stem cells (MSCs) transplantation for the treatment of cardiovascular disease is not satisfactory. The low viability and angiogenic capacity of the implanted cells in the local infarct tissues restrict their further application. Evidence shows that...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0861-x
更新日期：2018-04-19 00:00:00
abstract：BACKGROUND:Nicotinamide adenine dinucleotide (NAD+) is a critical molecule involved in various biological functions. Poly (ADP-ribose) polymerase 1 (PARP1) and sirtuin 1 (SIRT1) affect cellular NAD+ levels and play essential roles in regulating metabolism. However, there has been little research on the effects of PARP1...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0961-7
更新日期：2018-08-23 00:00:00
abstract：BACKGROUND:Alveolar bone loss is a frequent occurrence. Dental pulp stem cells (DPSCs) which have invasive accessibility and high osteogenic potential is a promising source for cell-based bone regeneration. EphrinB2 is involved in bone homeostasis and osteogenesis. The aim of this study was to investigate the effect an...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1540-2
更新日期：2020-01-29 00:00:00
abstract：BACKGROUND:Acute liver failure (ALF) is a complicated condition that is characterized by global hepatocyte death and often requires immediate liver transplantation. However, this therapy is limited by shortage of donor organs. Mesenchymal stem cells (MSCs) and hepatocytes are two attractive sources of cell-based therap...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01962-7
更新日期：2020-10-23 00:00:00
abstract：INTRODUCTION:Cell-based therapy represents a new frontier in the treatment of a wide variety of human diseases traditionally associated with morbidity outcomes, including those involving inflammation, autoimmunity, tissue damage, and cancer. However, the use of mesenchymal stem cells (MSCs) to treat multiple myeloma (M...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt322
更新日期：2013-01-01 00:00:00
abstract：INTRODUCTION:Nervous system injuries comprise a diverse group of disorders that include traumatic brain injury (TBI). The potential of mesenchymal stem cells (MSCs) to differentiate into neural cell types has aroused hope for the possible development of autologous therapies for central nervous system injury. METHODS:I...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt151
更新日期：2013-01-06 00:00:00
abstract：BACKGROUND:Mesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different sources, such as bone marrow (BM), umbilical cord tissue (UCT) and adipose tissue (AT), share many biolo...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-016-0383-3
更新日期：2016-08-25 00:00:00
abstract：INTRODUCTION:The therapeutic effects of mesenchymal stem cells (MSCs) have been limited by their apoptosis induced by oxidative stress after delivery into the injured sites. Therefore, strategies designed to improve the MSC therapeutic efficacy need to be explored. Tetramethylpyrazine (TMP) can promote the proliferatio...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01700-z
更新日期：2020-05-19 00:00:00
abstract：BACKGROUND:Spinal metastasis is a major challenge in patients with advanced lung cancer, but the mechanisms in the organotropism of metastasis are still unclear. Adipose-derived mesenchymal stem cells (ADSCs) exhibit cancer-promoting properties that influence the tumour microenvironment; however, there is no research o...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1280-3
更新日期：2019-06-13 00:00:00
abstract：BACKGROUND:WNT and TGFβ signaling pathways play critical regulatory roles in cardiomyocyte fate determination and differentiation. MiRNAs are also known to regulate different biological processes and signaling pathways. Here, we intended to find candidate miRNAs that are involved in cardiac differentiation through regu...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-019-1249-2
更新日期：2019-06-27 00:00:00
abstract：:Stem cell behaviors are regulated by multiple microenvironmental cues. As an external signal, mechanical stiffness of the extracellular matrix is capable of governing stem cell fate determination, but how this biophysical cue is translated into intracellular signaling remains elusive. Here, we elucidate mechanisms by ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-015-0083-4
更新日期：2015-05-27 00:00:00
abstract：INTRODUCTION:Fallopian tube, which is normally discarded in surgical procedures, has proven to be a source of mesenchymal stem cells (MSCs) with increasing evidence. However, fallopian tube mucosa, which can be acquired via non-invasive procedures, is a previously unknown source of MSCs. In the present study, we explor...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0094-1
更新日期：2015-05-21 00:00:00
abstract：BACKGROUND:Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal cells currently tested for multiple therapeutic purposes. Their potential to home into tumors, to secrete trophic/vasculogenic factors, and to suppress immune response raises questions regarding their biosafety. Our aim was to evaluate wheth...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0878-1
更新日期：2018-05-11 00:00:00
abstract：BACKGROUND:In recent years, adipose-derived stromal cells (ASCs) have been heavily studied for soft tissue regeneration, augmentation, and dermal wound healing. METHODS:In this review, we investigated the trends in injectable scaffolds for ASC delivery in the dermis, and injectable or implantable scaffolds for ASC del...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-020-02132-5
更新日期：2021-01-19 00:00:00
abstract：:Destruction or dysfunction of limbal epithelial stem cells (LESCs) leads to unilateral or bilateral limbal stem cell deficiency (LSCD). Fifteen years have passed since the first transplantation of ex vivo cultivated oral mucosal epithelial cells (COMET) in humans in 2004, which represents the first use of a cultured n...
journal_title：Stem cell research & therapy
pub_type： 杂志文章,评审
doi：10.1186/s13287-020-01783-8
更新日期：2020-07-21 00:00:00
abstract：:The advent of human induced pluripotent stem cells has been heralded as a major breakthrough in the study of pluripotent stem cells, for these cells have yielded fundamental insights into the reprogrammability of somatic cell fates, but also because of their seemingly great promise in applications, including potential...
journal_title：Stem cell research & therapy
pub_type： 社论
doi：10.1186/scrt9
更新日期：2010-04-12 00:00:00
abstract：OBJECTIVES:Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet. MATERIALS AND METHODS:Here, we developed the full chemical-defined medium, NBVbe med...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-01641-7
更新日期：2020-03-19 00:00:00
abstract：BACKGROUND:For patients with thromboangiitis obliterans (TAO), revascularization with bypass or angioplasty is frequently not feasible due to the poor outflow of the distal small vessels. We evaluated the long-term results of our experience treating patients with TAO with autologous bone marrow-derived mononuclear cell...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-018-0784-6
更新日期：2018-02-22 00:00:00
abstract：INTRODUCTION:Estrogen plays an important role in the osteogenic differentiation of mesenchymal stem cells, while stem cells from apical papilla (SCAP) can contribute to the formation of dentin/bone-like tissues. To date, the effects of estrogen on the differentiation of SCAP remain unclear. METHODS:SCAP was isolated a...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt515
更新日期：2014-11-17 00:00:00
abstract：INTRODUCTION:Transplanting mesenchymal stromal cells (MSCs) or their derivatives into a neurodegenerative environment is believed to be beneficial because of the trophic support, migratory guidance, immunosuppression, and neurogenic stimuli they provide. SB623, a cell therapy for the treatment of chronic stroke, curren...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/scrt418
更新日期：2014-02-26 00:00:00
abstract：:Evidence suggests that transdifferentiation of mesenchymal stem cells (MSCs) into various neuronal cells contributes to functional recovery after experimental spinal cord injury. Qiu et al. have recently published an exciting article in Stem Cell Research & Therapy demonstrating the transdifferentiation of already dif...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0186-y
更新日期：2015-10-05 00:00:00
abstract：BACKGROUND:Precision or individualized medicine is increasingly pervasive across medical and surgical disciplines. However, the concept has not been introduced in regenerative medicine. MAIN BODY:Targeted and engineered cellular and acellular therapy, specific to individuals or disease states, could significantly impr...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-020-02092-w
更新日期：2021-01-07 00:00:00
abstract：INTRODUCTION:Fetal bovine serum (FBS) is an animal product used as a medium supplement. The animal origin of FBS is a concern if cultured stem cells are to be utilized for human cell therapy. Therefore, a substitute for FBS is desirable. In this study, an industrial, xeno-free, pharmaceutical-grade supplement for cell ...
journal_title：Stem cell research & therapy
pub_type： 杂志文章
doi：10.1186/s13287-015-0016-2
更新日期：2015-03-13 00:00:00
