Abstract：
BACKGROUND:High invasion and metastasis are the primary factors causing poor prognosis of patients with hepatocellular carcinoma (HCC). However, the molecular mechanisms underlying these biological behaviors have not been completely elucidated. In this study, we investigate the molecular mechanism by which hypoxia promotes HCC invasion and metastasis through inducing epithelial-mesenchymal transition (EMT). METHODS:The expression of EMT markers was analyzed by immunohistochemistry. Effect of hypoxia on induction of EMT and ability of cell migration and invasion were performed. Luciferase reporter system was used for evaluation of Snail regulation by hypoxia-inducible factor -1α (HIF-1α). RESULTS:We found that overexpression of HIF-1α was observed in HCC liver tissues and was related to poor prognosis of HCC patients. HIF-1α expression profile was correlated with the expression levels of SNAI1, E-cadherin, N-cadherin and Vimentin. Hypoxia was able to induce EMT and enhance ability of invasion and migration in HCC cells. The same phenomena were also observed in CoCl2-treated cells. The shRNA-mediated HIF-1α suppression abrogated CoCl2-induced EMT and reduced ability of migration and invasion in HCC cells. Luciferase assay showed that HIF-1α transcriptional regulated the expression of SNAI1 based on two hypoxia response elements (HREs) in SNAI1 promoter. CONCLUSIONS:We demonstrated that hypoxia-stabilized HIF1α promoted EMT through increasing SNAI1 transcription in HCC cells. This data provided a potential therapeutic target for HCC treatment.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Zhang L,Huang G,Li X,Zhang Y,Jiang Y,Shen J,Liu J,Wang Q,Zhu J,Feng X,Dong J,Qian Cdoi
10.1186/1471-2407-13-108subject
Has Abstractpub_date
2013-03-09 00:00:00pages
108issn
1471-2407pii
1471-2407-13-108journal_volume
13pub_type
杂志文章相关文献
BMC CANCER文献大全abstract：BACKGROUND:We studied the cases of single and multiple HPV infection and analyzed the correlation with negative cases, and preneoplastic and neoplastic lesions of the uterine cervix with the aim of making a contribution to the prognostic factor under discussion. METHODS:Nine hundred nine women undergoing second level ...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-020-06946-7
更新日期：2020-05-19 00:00:00
abstract：BACKGROUND:Lifestyle factors, including food and nutrition, physical activity, body composition and reproductive factors, and single nucleotide polymorphisms (SNPs) are associated with breast cancer risk, but few studies of these factors have been performed in the Japanese population. Thus, the goals of this study were...
journal_title：BMC cancer
pub_type： 杂志文章,多中心研究
doi：10.1186/1471-2407-13-565
更新日期：2013-12-01 00:00:00
abstract：BACKGROUND:Although Temozolomide is effective against glioblastoma, the prognosis remains dismal and new regimens with synergistic activity are sought for. METHODS:In this phase-I/II trial, pegylated liposomal doxorubicin (Caelyx, PEG-Dox) and prolonged administration of Temozolomide in addition to radiotherapy was in...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-9-308
更新日期：2009-09-02 00:00:00
abstract：BACKGROUND:Increased mammographic breast density is a moderate risk factor for breast cancer. Different scales have been proposed for classifying mammographic density. This study sought to assess intra-rater agreement for the most widely used scales (Wolfe, Tabár, BI-RADS and Boyd) and compare them in terms of classify...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-10-485
更新日期：2010-09-13 00:00:00
abstract：BACKGROUND:We examined 15 years of key performance indicators (KPIs) of the population-based mammography screening programme (PMSP) in Flanders, Belgium. METHODS:Individual screening data were linked to the national cancer registry to obtain oncological follow-up. We benchmarked crude KPI results against KPI-targets s...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-019-6230-z
更新日期：2019-10-28 00:00:00
abstract：BACKGROUND:RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients. METHODS:Microvesicle RNA from serum from patients with de-novo primary glioblastoma multiforme (N = 9...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-12-22
更新日期：2012-01-17 00:00:00
abstract：BACKGROUND:Wilms' tumor is also called nephroblastoma and is the most common pediatric renal cancer. Several genetic and epigenetic factors have been found to account for the development of Wilms' tumor. MiRNAs play important roles in this tumorigenic process. In the present study, we aimed to investigate the role of m...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-019-5609-1
更新日期：2019-04-29 00:00:00
abstract：BACKGROUND:HIV infection is associated with a higher incidence of precancerous cervical lesions and their progression to invasive cervical cancer (ICC). Zambia is a global epicenter of HIV and ICC, yet the overall burden of cervical pre-cancer [cervical intraepithelial neoplasia 3 (CIN3)] and ICC among its HIV positive...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-015-1558-5
更新日期：2015-07-24 00:00:00
abstract：BACKGROUND:In Scotland colorectal cancer (CRC) is the third most common cancer and a leading cause of cancer death. Epidemiological studies have reported conflicting associations between statins and CRC risk and there is one published report of the association between statins and CRC survival. METHODS:Analysis was car...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-12-487
更新日期：2012-10-22 00:00:00
abstract：BACKGROUND:Colorectal cancer screening is key to early detection and thus to early treatment, but uptake is often sub-optimal, particularly amongst lower income groups. It is proposed that the imminent introduction of the single-sample Faecal Immunochemical Test (FIT) in Scotland may lead to increased uptake as compare...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-016-2133-4
更新日期：2016-02-13 00:00:00
abstract：BACKGROUND:It is often difficult to diagnose inflammatory bowel disease (IBD)-associated neoplasia endoscopically due to background inflammation. In addition, due to the absence of sensitive tumor biomarkers, countermeasures against IBD-associated neoplasia are crucial. The purpose of this study is to develop a new dia...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-020-07699-z
更新日期：2020-12-03 00:00:00
abstract：BACKGROUND:Previous studies on bladder cancer have shown nodal involvement to be an independent indicator of prognosis and survival. This study aimed at developing an objective method for detection of nodal metastasis from molecular profiles of primary urothelial carcinoma tissues. METHODS:The study included primary b...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-6-159
更新日期：2006-06-16 00:00:00
abstract：BACKGROUND:Following resection of liver metastases from colorectal cancer, 5-year survivals are reportedly 30 - 39%. It can be assumed that this clinical situation represents systemic disease. Therefore, it is postulated that systemic chemotherapy would improve outcomes, particularly in those whose disease is sensitive...
journal_title：BMC cancer
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1186/1471-2407-4-32
更新日期：2004-07-10 00:00:00
abstract：BACKGROUND:Population attributable risks (PARs) are useful tool to estimate the burden of risk factors in cancer incidence. Few studies estimated the PARs of oral cavity cancer to tobacco smoking alone, alcohol drinking alone and their joint consumption but none performed analysis stratified by subsite, gender or age. ...
journal_title：BMC cancer
pub_type： 杂志文章,多中心研究
doi：10.1186/s12885-015-1841-5
更新日期：2015-10-31 00:00:00
abstract：BACKGROUND:MicroRNA (miR)-200c has been widely reported to be involved in colon cancer progress. However, the mechanisms of miR-200c in regulating tumor metastasis and growth remain to be fully elucidated. This study aimed to investigate the mechanism of miR-200c targets fucosyltransferase 4 (FUT4) on the proliferation...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-020-07670-y
更新日期：2021-01-04 00:00:00
abstract：BACKGROUND:CD73 is one of the critical component in the formation of immunosuppressive microenvironment in cancers. We aimed to provide an overview of the current status of CD73 expression and its relationship with clinicopathlogical features and prognosis in different cancers. METHODS:PubMed, Web of Science, EMBASE a...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-018-4073-7
更新日期：2018-03-07 00:00:00
abstract：BACKGROUND:Gynecologic cancer is one of the most common malignant diseases in working-age women. This study investigated whether several characteristics influence return to work after treatment of gynecologic cancer. METHODS:We investigated the correlations between return to work and several other characteristics in 1...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-016-2627-0
更新日期：2016-07-29 00:00:00
abstract：BACKGROUND:As a key gene in the immunosurveillance of cell malignancy, Cytotoxic T-lymphocyte antigen 4 (CTLA-4 is an important negative regulator of T cell activation and proliferation. The CTLA-4 +49G > A polymorphism is one of the most commonly studied polymorphisms in this gene due to its association with cancer ri...
journal_title：BMC cancer
pub_type： 杂志文章,meta分析
doi：10.1186/1471-2407-10-522
更新日期：2010-10-04 00:00:00
abstract：BACKGROUND:Temozolomide (TMZ)-induced side effects and drug tolerance to human gliomas are still challenging issues now. Our previous studies showed that honokiol, a major bioactive constituent of Magnolia officinalis (Houpo), is safe for normal brain cells and can kill human glioma cells. This study was further aimed ...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-018-4267-z
更新日期：2018-04-03 00:00:00
abstract：BACKGROUND:Elucidating the molecular mechanisms by which tumors become resistant to Herceptin is critical for the treatment of Her2-overexpressed metastatic breast cancer. METHODS:To further understand Herceptin resistance mechanisms at the molecular level, we used comparative proteome approaches to analyze two human ...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-8-286
更新日期：2008-10-04 00:00:00
abstract：BACKGROUND:Prostate tumours are commonly poorly oxygenated which is associated with tumour progression and development of resistance to chemotherapeutic drugs and radiotherapy. Fibroblast growth factor 8b (FGF8b) is a mitogenic and angiogenic factor, which is expressed at an increased level in human prostate tumours an...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-10-596
更新日期：2010-10-30 00:00:00
abstract：BACKGROUND:Exercise has favorable effects on biomarkers associated with a lower risk of breast cancer, however it is unclear if higher doses of exercise provide additional effects. No clinical trial has systematically examined how different exercise volumes influence the mechanisms underlying breast cancer etiology. Th...
journal_title：BMC cancer
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1186/1471-2407-14-919
更新日期：2014-12-06 00:00:00
abstract：BACKGROUND:Adoptive T-cell therapy relying on conventional T cells transduced with T-cell receptors (TCRs) or chimeric antigen receptors (CARs) has caused substantial tumor regression in several clinical trials. However, genetically engineered T cells have been associated with serious side-effects due to off-target tox...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-017-3539-3
更新日期：2017-08-17 00:00:00
abstract：BACKGROUND:There has been a sharp rise in the incidence of human papillomavirus (HPV) associated oropharyngeal squamous cell carcinoma (OPSCC) in many countries. Patients with HPV-positive OPSCC have a more favorable prognosis compared with HPV-negative OPSCC, leading to investigation and adoption of de-escalation trea...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-019-6266-0
更新日期：2019-11-06 00:00:00
abstract：INTRODUCTION:Metastasis represents a major adverse step in the progression of breast carcinoma. Lymph node invasion is the most relevant prognostic factor; however little is known on the molecular events associated with lymph node metastasis process. This study is to investigate the status and role of methylation in ly...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-10-378
更新日期：2010-07-20 00:00:00
abstract：BACKGROUND:CST6 promoter is highly methylated in cancer, and its detection can provide important prognostic information in breast cancer patients. The aim of our study was to develop a Methylation-Sensitive High Resolution Melting Analysis (MS-HRMA) assay for the investigation of CST6 promoter methylation. METHODS:We ...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-12-486
更新日期：2012-10-22 00:00:00
abstract：BACKGROUND:Breast cancer can impact survivors in many aspects of their life. Scarce information is currently available on the quality of life of cancer survivors in Bahrain. The objective of this study is to describe the quality of life of Bahraini women with breast cancer and its association with their sociodemographi...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/1471-2407-13-212
更新日期：2013-04-28 00:00:00
abstract：BACKGROUND:The aim of this study was to compare surgical treatment received by Aboriginal and non-Aboriginal people with non-small cell lung cancer (NSCLC) in New South Wales (NSW), Australia and to examine whether patient and disease characteristics are associated with any disparities found. An additional objective wa...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-016-2322-1
更新日期：2016-04-25 00:00:00
abstract：BACKGROUND:The survival following transarterial chemoembolization (TACE) alone is still low in unresectable hepatocellular carcinoma (HCC) with almost patients developing disease progression after treatment. There is need to investigate additional therapeutic options that would intensify the initial response to TACE. T...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-016-2894-9
更新日期：2016-11-03 00:00:00
abstract：BACKGROUND:Colorectal cancer (CRC) patients with metastatic disease can become cured if neoadjuvant treatment can enable a resection. The search for predictive biomarkers is often performed on primary tumours tissue. In order to assess the effectiveness of tailored treatment in regard to the primary tumour the differen...
journal_title：BMC cancer
pub_type： 杂志文章
doi：10.1186/s12885-018-4639-4
更新日期：2018-07-20 00:00:00
