The effect of cytokine mediators on prostaglandin inhibition by human decidual cells.

Abstract:

OBJECTIVE:The purpose of this study was to determine the inhibitory effects of anti-interleukin-1beta and transforming growth factor-beta2 on the production of interleukin-1beta and prostaglandin E2 by human decidual cells. STUDY DESIGN:Decidual membranes were obtained from 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopolysaccharide as a control, lipopolysaccharide plus anti-interleukin-1beta, and lipopolysaccharide plus transforming growth factor-beta2, respectively. Interleukin-1beta and prostaglandin E2 were measured by enzyme-linked immunosorbent assay and radioimmunoassay. RESULTS:At the end of a 24-hour culture with lipopolysaccharide plus anti-interleukin-1beta, anti-interleukin-1beta decreased the lipopolysaccharide-induced prostaglandin E2 production significantly at the concentrations of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P < .05) and also decreased the lipopolysaccharide-induced interleukin-1beta production significantly at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P < .05). In the medium containing lipopolysaccharide plus transforming growth factor-beta2, transforming growth factor-beta2 also decreased the lipopolysaccharide-induced prostaglandin E2 production significantly at the concentrations of 1 ng/mL and 10 ng/mL (P < .05) but increased the lipopolysaccharide-induced interleukin-1beta production significantly at the concentration of 1 ng/mL (P < .05). CONCLUSIONS:Lipopolysaccharide-induced prostaglandin E2 production by human decidual cells in vitro may be prevented by anti-interleukin-1beta and transforming growth factor-beta2, and interleukin-1beta production may be prevented by anti-interleukin-1beta.

journal_name

Am J Obstet Gynecol

authors

Kim YJ,Ahn JJ,Woo BH

doi

10.1016/s0002-9378(98)70265-0

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

146-9

issue

1

eissn

0002-9378

issn

1097-6868

pii

S0002937898702650

journal_volume

179

pub_type

临床试验,杂志文章
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    pub_type: 杂志文章

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    pub_type: 杂志文章

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    pub_type: 杂志文章

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    pub_type: 杂志文章

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    更新日期:1978-02-15 00:00:00

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    更新日期:2007-09-01 00:00:00

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    更新日期:2000-02-01 00:00:00

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