Studies of phospholipid hydration by high-resolution magic-angle spinning nuclear magnetic resonance.

abstract：:We present a theoretical and experimental analysis of the effects of nonlinear flow in a cone-plate viscometer. The analysis predicts that flow in the viscometer is a function of two parameters, the Reynolds number and the cone angle. Nonlinear flow occurs at high shear rates and causes spatial variations in wall shea...

journal_title：Biophysical journal

authors： Shankaran H,Neelamegham S

更新日期：2001-06-01 00:00:00

abstract：:Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction limit emit separately when simultaneously exposed to excitation light. A basic mechanism for such transient fluo...

journal_title：Biophysical journal

authors： Testa I,Wurm CA,Medda R,Rothermel E,von Middendorf C,Fölling J,Jakobs S,Schönle A,Hell SW,Eggeling C

更新日期：2010-10-20 00:00:00

abstract：:Red blood cell membrane exhibits a large resistance to changes in surface area. This resistance is characterized by the area expansivity modulus K, which relates the isotropic membrane force resultant, T, to the fractional change in membrane surface area delta A/Ao. The experimental technique commonly used to determin...

journal_title：Biophysical journal

authors： Katnik C,Waugh R

更新日期：1990-04-01 00:00:00

abstract：:Polymers play an important role in many biological systems, so a fundamental understanding of their cross-links is crucial not only for the development of medicines but also for the development of biomimetic materials. The biomechanical movements of all mammals are aided by tendon fibrils. The self-organization and bi...

journal_title：Biophysical journal

authors： Gutsmann T,Hassenkam T,Cutroni JA,Hansma PK

更新日期：2005-07-01 00:00:00

abstract：:Myosin II is a central mechanoenzyme in a wide range of cellular morphogenic processes. Its cellular localization is dependent not only on signal transduction pathways, but also on mechanical stress. We suggest that this stress-dependent distribution is the result of both the force-dependent binding to actin filaments...

journal_title：Biophysical journal

authors： Luo T,Mohan K,Srivastava V,Ren Y,Iglesias PA,Robinson DN

更新日期：2012-01-18 00:00:00

abstract：:We compared wild-type rat skeletal muscle NaChs (micro1) and a mutant NaCh (Y1586K) that has a single amino acid substitution, lysine (K) for tyrosine (Y), at position 1586 in the S6 transmembrane segment of domain 4. In Y1586K, macroscopic current decay is faster, the V(1/2) of the activation curve is shifted in the ...

journal_title：Biophysical journal

authors： O'Reilly JP,Wang SY,Wang GK

更新日期：2000-02-01 00:00:00

abstract：:The properties of vesicle membranes prepared from 16:0-SM, 16:0-DHSM, or DPPC were characterized using steady-state and time-resolved fluorescence spectroscopy and different fluorescent reporter molecules. The acyl-chain region was probed using free and phospholipid-bound 1,6-diphenyl-1,3,5-hexatriene. 16:0-DHSM was f...

journal_title：Biophysical journal

authors： Nyholm T,Nylund M,Söderholm A,Slotte JP

更新日期：2003-02-01 00:00:00

abstract：:The equation of motion for sperm flagella, in which the elastic bending moment and the active contractile moment are balanced by the moment from the viscous resistance of the surrounding fluid, is solved for a wave solution that superimposes partial solutions. Substitution of the expression for the wave solution into ...

journal_title：Biophysical journal

authors： Rikmenspoel R

更新日期：1978-08-01 00:00:00

abstract：:Single-stranded junctions/loops are frequently occurring structural motifs in nucleic acid structures. Due to the polyanionic nature of the nucleic acid backbone, metal ions play a crucial role in the loop stability. Here we use the tightly bound ion theory, which can account for the possible ion correlation and ensem...

journal_title：Biophysical journal

authors： Tan ZJ,Chen SJ

更新日期：2008-07-01 00:00:00

abstract：:We introduce here a novel Monte Carlo simulation method for studying the interactions of hydrophobic peptides with lipid membranes. Each of the peptide's amino acids is represented as two interaction sites: one corresponding to the backbone alpha-carbon and the other to the side chain, with the membrane represented as...

journal_title：Biophysical journal

authors： Kessel A,Shental-Bechor D,Haliloglu T,Ben-Tal N

更新日期：2003-12-01 00:00:00

abstract：:The biophysical characterization of nonfunctional protein aggregates at physiologically relevant temperatures is much needed to gain deeper insights into the kinetic and thermodynamic relationships between protein folding and misfolding. Dynamic and static laser light scattering have been employed for the detection an...

journal_title：Biophysical journal

authors： Chow C,Kurt N,Murphy RM,Cavagnero S

更新日期：2006-01-01 00:00:00

abstract：:Calmodulin (CaM) expression is upregulated upon HIV-1 infection and interacts with proteins involved in viral processing, including the multifunctional HIV-1 MA protein. We present here the results of studies utilizing small-angle neutron scattering with contrast variation that, when considered in the light of earlier...

journal_title：Biophysical journal

authors： Taylor JE,Chow JYH,Jeffries CM,Kwan AH,Duff AP,Hamilton WA,Trewhella J

更新日期：2012-08-08 00:00:00

abstract：:We report here a method for the determination of the pKa of histidine in complex or heterogeneous systems amenable to neither solid-state nor solution NMR spectroscopy. Careful synthesis of a fluorenylmethyloxycarbonyl- and trityl-protected, C2-deuterated histidine produces a vibrational-probe-equipped amino acid that...

journal_title：Biophysical journal

authors： Braun GA,Pogostin BH,Pucetaite M,Londergan CH,Åkerfeldt KS

更新日期：2020-11-03 00:00:00

abstract：:A number of properties of certain living embryonic tissues can be explained by considering them as liquids. Tissue fragments left in a shaker bath round up to form spherical aggregates, as do liquid drops. When cells comprising two distinct embryonic tissues are mixed, typically a nucleation-like process takes place, ...

journal_title：Biophysical journal

authors： Forgacs G,Foty RA,Shafrir Y,Steinberg MS

更新日期：1998-05-01 00:00:00

abstract：:Clusters of calcium-loaded sarcoplasmic reticulum (SR) vesicles in agarose gel were previously shown to behave as an excitable medium that propagates calcium waves. In a 3D-hexagonal disposition, the distance between neighboring spheres (which may stand for SR vesicles) is constant and the relationship between distanc...

journal_title：Biophysical journal

authors： Wussling MH,Aurich I,Knauf O,Podhaisky H,Holzhausen HJ

更新日期：2004-12-01 00:00:00

abstract：:Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed the biophysical prop...

journal_title：Biophysical journal

authors： Yurlova L,Kahya N,Aggarwal S,Kaiser HJ,Chiantia S,Bakhti M,Pewzner-Jung Y,Ben-David O,Futerman AH,Brügger B,Simons M

更新日期：2011-12-07 00:00:00

abstract：:Using polarography and suitable mathematical analysis, the oxygen permeability of an individual layer of a bilayered tissue may be determined if the oxygen consumption and permeability of the other layer are known. Results obtained on rabbit cornea indicate that the permeability of the epithelium is about four times t...

journal_title：Biophysical journal

authors： Freeman RD,Fatt I

更新日期：1972-03-01 00:00:00

abstract：:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipet...

journal_title：Biophysical journal

authors： Shevchuk A,Tokar S,Gopal S,Sanchez-Alonso JL,Tarasov AI,Vélez-Ortega AC,Chiappini C,Rorsman P,Stevens MM,Gorelik J,Frolenkov GI,Klenerman D,Korchev YE

更新日期：2016-05-24 00:00:00

abstract：:Human posttranslationally modified N-ras oncogenes are known to be implicated in numerous human cancers. Here, we applied a combination of experimental and computational techniques to determine structural and dynamical details of the lipid chain modifications of an N-ras heptapeptide in 1,2-dimyristoyl-sn-glycero-3-ph...

journal_title：Biophysical journal

authors： Vogel A,Tan KT,Waldmann H,Feller SE,Brown MF,Huster D

更新日期：2007-10-15 00:00:00

abstract：:The structure of dynamic states in biological networks is of fundamental importance in understanding their function. Considering the elementary reaction structure of reconstructed metabolic networks, we show how appreciation of a gradient matrix, G =dv/dx (where v is the vector of fluxes and x is the vector of concent...

journal_title：Biophysical journal

authors： Jamshidi N,Palsson BØ

更新日期：2009-09-02 00:00:00

abstract：:Chromatin is partitioned on multiple length scales into subcompartments that differ from each other with respect to their molecular composition and biological function. It is a key question how these compartments can form even though diffusion constantly mixes the nuclear interior and rapidly balances concentration gr...

journal_title：Biophysical journal

authors： Erdel F,Rippe K

更新日期：2018-05-22 00:00:00

abstract：:This paper describes an analysis of microscopic models for the coupling between ion flow and rotation of bacterial flagella. In model I it is assumed that intersecting half-channels exist on the rotor and the stator and that the driving ion is constrained to move together with the intersection site. Model II is based ...

journal_title：Biophysical journal

authors： Läuger P

更新日期：1988-01-01 00:00:00

abstract：:The enzyme F1-adenosine triphosphatase (ATPase) is a molecular motor that converts the chemical energy stored in the molecule adenosine triphosphate (ATP) into mechanical rotation of its gamma-subunit. During steady-state catalysis, the three catalytic sites of F1 operate in a cooperative fashion such that at every in...

journal_title：Biophysical journal

authors： Dittrich M,Hayashi S,Schulten K

更新日期：2004-11-01 00:00:00

abstract：:We have used magnetic relaxation dispersion to study bovine pancreatic trypsin inhibitor (BPTI) self-association as a function of pH, salt type and concentration, and temperature. The magnetic relaxation dispersion method sensitively detects stable oligomers without being affected by other interactions. We find that B...

journal_title：Biophysical journal

authors： Gottschalk M,Venu K,Halle B

更新日期：2003-06-01 00:00:00

abstract：:Impact loading of articular cartilage causes extensive chondrocyte death. Cell membranes have a limited elastic range of 3-4% strain but are protected from direct stretch during physiological loading by their membrane reservoir, an intricate pattern of membrane folds. Using a finite-element model, we suggested previou...

journal_title：Biophysical journal

authors： Moo EK,Amrein M,Epstein M,Duvall M,Abu Osman NA,Pingguan-Murphy B,Herzog W

更新日期：2013-10-01 00:00:00

abstract：:Excitation-contraction coupling in atrial cells is mediated by calcium (Ca) signaling between L-type Ca channels and Ryanodine receptors that occurs mainly at the cell boundary. This unique architecture dictates essential aspects of Ca signaling under both normal and diseased conditions. In this study we apply laser s...

journal_title：Biophysical journal

authors： Shiferaw Y,Aistrup GL,Wasserstrom JA

更新日期：2017-08-08 00:00:00

abstract：:The electrogenic transport of ATP and ADP by the mitochondrial ADP/ATP carrier (AAC) was investigated by recording transient currents with two different techniques for performing concentration jump experiments: 1) the fast fluid injection method: AAC-containing proteoliposomes were adsorbed to a solid supported membra...

journal_title：Biophysical journal

authors： Gropp T,Brustovetsky N,Klingenberg M,Müller V,Fendler K,Bamberg E

更新日期：1999-08-01 00:00:00

abstract：:The mating pathway in Saccharomyces cerevisiae is one of the best understood signal transduction pathways in eukaryotes. It transmits the mating signal from plasma membrane into the nucleus through the G-protein coupled receptor and the mitogen-activated protein kinase (MAPK) cascade. According to current understandin...

journal_title：Biophysical journal

authors： Shao D,Zheng W,Qiu W,Ouyang Q,Tang C

更新日期：2006-12-01 00:00:00

abstract：:Single-molecule studies allow the study of subtle activity differences due to local folding in proteins, but are time consuming and difficult because only a few molecules are observed in one experiment. We developed an assay where we can simultaneously measure the activity of hundreds of individual molecules. The assa...

journal_title：Biophysical journal

authors： Lee AI,Brody JP

更新日期：2005-06-01 00:00:00

abstract：:The vessel wall experiences progressive stiffening with age and the development of cardiovascular disease, which alters the micromechanical environment experienced by resident vascular smooth muscle cells (VSMCs). In vitro studies have shown that VSMCs are sensitive to substrate stiffness, but the exact molecular mech...

journal_title：Biophysical journal

authors： Sazonova OV,Lee KL,Isenberg BC,Rich CB,Nugent MA,Wong JY

更新日期：2011-08-03 00:00:00