Abstract：
:Anandamide and 2-arachidonoylglycerol (2-AG) are two endogenous ligands for the cannabinoid receptors, and their cannabimimetic activities are lost when they are hydrolyzed enzymatically. Cytosol and particulate fractions of porcine brain exhibited a high 2-AG hydrolyzing activity of 100 nmol/min/mg protein. Most of the activity could be attributed to a monoacylglycerol lipase-like enzyme that did not hydrolyze anandamide. It was separated by hydroxyapatite chromatography from anandamide amidohydrolase, which is also capable of hydrolyzing 2-AG as well as anandamide. Thus, porcine brain has at least two enzymes capable of hydrolyzing 2-AG. The 2-AG hydrolase activities of both the cytosolic and particulate enzymes were irreversibly and time-dependently inhibited by methyl arachidonyl fluorophosphonate with IC50 values as low as 2-3 nM.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Goparaju SK,Ueda N,Taniguchi K,Yamamoto Sdoi
10.1016/s0006-2952(98)00314-1subject
Has Abstractpub_date
1999-02-15 00:00:00pages
417-23issue
4eissn
0006-2952issn
1873-2968pii
S0006295298003141journal_volume
57pub_type
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