Abstract:
:The major metabolic products of the endogeneous catecholamine dopamine are its 3- and 4-O-sulphates which have also been implicated as intermediates in noradrenaline biosynthesis. Because of the unsatisfactory status of the literature concerning the synthesis, isolation, purity and characterisation of the dopamine O-sulphates we describe both a one-step synthesis and definitive separation and characterisation procedures for these metabolites. High-performance liquid chromatography (HPLC) combined with high-field nuclear magnetic resonance techniques were employed. The chemical sulphonation of dopamine gave three synthetic products, whose relative amounts depended critically upon the reaction conditions employed. Dopamine 3- and 4-O-sulphates together with dopamine 6-sulphonic acid, a hitherto undescribed derivative of dopamine, were for the first time isolated and characterised unequivocally. It should now prove possible to reappraise critically the biological significance of the major metabolite products of dopamine.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Osikowska BA,Idle JR,Swinbourne FJ,Sever PSdoi
10.1016/0006-2952(82)90115-0subject
Has Abstractpub_date
1982-07-01 00:00:00pages
2279-84issue
13eissn
0006-2952issn
1873-2968pii
0006-2952(82)90115-0journal_volume
31pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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