Abstract:
:Complex I is the largest of the mitochondrial respiratory chain proteins, and contains subunits encoded by both mitochondrial and nuclear genomes. Leber's hereditary optic neuropathy has been clearly linked to mutations of mitochondrial DNA complex I genes, and variable complex I functional defects have been reported. We have confirmed an approximate 60% defect in mitochondrial NADH CoQ1 reductase activity in cultured fibroblasts bearing the 3460-bp G to A mutation within the ND1 gene. However complex I-linked ATP synthesis was found to be normal in these fibroblasts. A 60% rotenone-induced decrease in complex I activity was shown to reduce ATP synthesis in normal fibroblasts, indicating that this level of complex I activity was below the threshold required to affect ATP synthesis. Although 3460 LHON mitochondria were less sensitive to rotenone inhibition, this did not explain the decreased complex I activity as the rotenone insensitive activity was not increased, nor did the inhibitor diphenyleneiodonium inhibit the NADH CoQ1 reductase activity to a greater extent. Decreased NADH cytochrome c reductase activity in cybrids homoplasmic for the 3460 LHON mtDNA mutation confirmed that the decrease in complex I activity was not specific to the assay used and was not caused by inhibitory effects of ubiquinone analogues used in the NADH CoQ1 reductase assay. These findings have important implications for our understanding of complex I dysfunction in the pathogenesis of 3460 Leber's hereditary optic neuropathy.
journal_name
J Neurol Scijournal_title
Journal of the neurological sciencesauthors
Cock HR,Cooper JM,Schapira AHdoi
10.1016/s0022-510x(99)00088-xsubject
Has Abstractpub_date
1999-05-01 00:00:00pages
10-7issue
1eissn
0022-510Xissn
1878-5883pii
S0022-510X(99)00088-Xjournal_volume
165pub_type
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journal_title:Journal of the neurological sciences
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journal_title:Journal of the neurological sciences
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doi:10.1016/s0022-510x(98)00327-x
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journal_title:Journal of the neurological sciences
pub_type: 临床试验,杂志文章
doi:10.1016/0022-510x(95)00016-u
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/j.jns.2007.10.029
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
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journal_title:Journal of the neurological sciences
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pub_type: 杂志文章
doi:10.1016/j.jns.2017.03.015
更新日期:2017-05-15 00:00:00
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pub_type: 杂志文章
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更新日期:2009-07-15 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/0022-510x(77)90190-3
更新日期:1977-08-01 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/0022-510x(76)90103-9
更新日期:1976-06-01 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
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更新日期:2008-06-15 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/j.jns.2015.01.014
更新日期:2015-02-15 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章,评审
doi:10.1016/j.jns.2009.09.009
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章,评审
doi:10.1016/j.jns.2005.11.012
更新日期:2006-03-15 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/s0022-510x(98)00050-1
更新日期:1998-04-15 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/j.jns.2011.03.004
更新日期:2011-06-15 00:00:00
abstract::A multidimensional quantitative EMG analysis method with averaged motor unit potentials (MUPs) was applied to a study of different stages of polymyositis. The duration parameters had a general tendency to increase in polymyositis. The only exception was the total MUP duration, which decreased in the onset stage of acu...
journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/0022-510x(82)90029-6
更新日期:1982-12-01 00:00:00
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journal_title:Journal of the neurological sciences
pub_type: 杂志文章
doi:10.1016/j.jns.2014.03.043
更新日期:2014-06-15 00:00:00