Abstract：
:We have previously demonstrated that daunorubicin (DNR) induces apoptosis in some leukemic myeloid cell lines. We investigated a potential protective role for Bcl-2 in apoptosis induced by DNR in two leukemic cell lines, one myeloid and one lymphoid, overexpressing the anti-apoptotic gene Bcl-2. Parental cells treated with DNR exhibited classical features of apoptosis 6 h after drug exposure, all the cells being dead after 30-48 h. In contrast, overexpression of Bcl-2 significantly delayed, but did not prevent the occurrence of DNR-induced apoptosis, with no surviving cells 96 h after drug exposure. To elucidate the mechanism of the protection mediated by Bcl-2, we explored the signaling pathway which initiates DNR-induced apoptosis. In this report, we show that, in both the myeloid and lymphoid parental cell lines, DNR triggered a sphingomyelin (SM) hydrolysis after 10-15 min with a concomitant ceramide generation. Moreover, exogenous ceramide induced DNA fragmentation in these cells, with levels similar to those observed with DNR treatment. In contrast, Bcl-2 overexpression protected the cells against apoptosis induced by ceramide treatment, without preventing the early SM hydrolysis nor the ceramide generation in these cells. Our results strongly suggest that Bcl-2-mediated protection of DNR-induced apoptosis is effected downstream of the SM-ceramide signaling pathway.
journal_name
Oncogenejournal_title
Oncogeneauthors
Allouche M,Bettaieb A,Vindis C,Rousse A,Grignon C,Laurent Gdoi
10.1038/sj.onc.1201023subject
Has Abstractpub_date
1997-04-17 00:00:00pages
1837-45issue
15eissn
0950-9232issn
1476-5594journal_volume
14pub_type
杂志文章相关文献
ONCOGENE文献大全abstract：:The phosphatase Cdc25A was shown to be a target of the transcription factor c-Myc. Myc-induced apoptosis appeared dependent on Cdc25A expression and Cdc25A over-expression could substitute for Myc-triggered apoptosis. These findings suggested that an important downstream component of Myc-mediated apoptosis was identif...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1204499
更新日期：2001-07-27 00:00:00
abstract：:Expression of neurotrophin receptors of the tyrosine kinase receptor (Trk) family is an important prognostic factor in solid tumors including neuroblastoma. High expression of TrkA (NTRK1) is associated with a favorable biology and outcome of neuroblastoma, whereas TrkB (NTRK2) is expressed on aggressive neuroblastoma...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1208000
更新日期：2005-01-06 00:00:00
abstract：:Mutations of SMAD4/DPC4 are found in about 60% of human invasive pancreatic ductal adenocarcinomas (PDACs); yet, the manner in which SMAD4 deficiency enhances tumorigenesis remains elusive. Using a Cre-LoxP approach, we generated a mutant mouse carrying a targeted deletion of Smad4 in the pancreas. We showed that the ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2009.375
更新日期：2010-02-04 00:00:00
abstract：:The proangiogenic cytokine Interleukin-3 (IL-3) is released by inflammatory cells in breast and ovarian cancer tissue microenvironments and also acts as an autocrine factor for human breast and kidney tumor-derived endothelial cells (TECs). We have previously shown that IL-3-treated endothelial cells (ECs) release ext...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-017-0034-x
更新日期：2018-03-01 00:00:00
abstract：:The study and comprehension of the molecular mechanisms underlying cancer biology strongly rely on mouse modeling. An ideal mouse model should have molecular, histopathological, and etiological characteristics as close as possible to those of the corresponding human tumors. Among solid tumors, colorectal cancer (CRC) ...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/s41388-018-0155-x
更新日期：2018-05-01 00:00:00
abstract：:Molecular, functional, and clinical analyses strongly suggest that chromosome segment 11p15.5 contains a gene involved in lung cancer pathogenesis. The critical region of allele loss is 310 kb in size. We used our contig of P1-phage artificial chromosome (PAC) clones together with newly identified bacterial artificial...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1205027
更新日期：2001-12-06 00:00:00
abstract：:Constitutive activation of the signal transducer and activator of transcription 3 (Stat3) and mutation of the p53 are both commonly detected in human prostate cancer cells. We sought to investigate whether there is functional regulation of Stat3 by wild-type (wt) p53. Our results demonstrate that expression of wt p53 ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1205426
更新日期：2002-05-02 00:00:00
abstract：:Induction of interstrand crosslinks (ICLs) in chromosomal DNA is considered a major reason for the antiproliferative effect of psoralen plus ultraviolet A (PUVA). It is unclear as to whether PUVA-induced cell cycle arrest is caused by ICLs mechanically stalling replication forks or by triggering cell cycle checkpoints...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1206613
更新日期：2003-09-18 00:00:00
abstract：:Recently the rat and mouse Growth Factor Independence (Gfi-1) genes have been cloned (Gilks et al., 1993; Zoring et al; 1996). This gene allows cells in culture to overcome the depletion of growth factors in the culture medium and maintain their proliferative potential. As part of a cloning strategy to isolated genes ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1200910
更新日期：1997-02-27 00:00:00
abstract：:The p21WAF1/CIP1/SDI1 gene is an important regulator of crucial cellular processes, including cell cycle control, cellular differentiation, and the response to genotoxic stress. Induction of p21 gene expression upon DNA damage is widely believed to be p53-dependent. In the present study we analysed the expression of p...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1201995
更新日期：1998-08-13 00:00:00
abstract：:Non-small-cell lung cancer (NSCLC) represents the most frequent and therapy-refractive sub-class of lung cancer. Improving apoptosis induction in NSCLC represents a logical way forward in treating this tumor. Cisplatin, a commonly used therapeutic agent in NSCLC, induces activation of N-terminal-c-Jun kinase (JNK) tha...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1209364
更新日期：2006-06-01 00:00:00
abstract：:Rapamycin, a natural product inhibitor of the Raptor-mammalian target of rapamycin complex (mTORC1), is known to induce Protein kinase B (Akt/PKB) Ser-473 phosphorylation in a subset of human cancer cell lines through inactivation of S6K1, stabilization of insulin receptor substrate (IRS)-1, and increased signaling th...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1210343
更新日期：2007-08-16 00:00:00
abstract：:Cellular adhesion to extracellular matrix is a central phenomenon for the maintenance of tissue integrity and cellular movement. Collectively, these processes are regulated by a fine-tuned balance between the formation and loosening of adhesive contacts, a process involving integrins, and the elevation and diminution ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1210883
更新日期：2008-04-10 00:00:00
abstract：:Reversible acetylation on protein lysine residues has been shown to regulate the function of both nuclear proteins such as histones and p53 and cytoplasmic proteins such as alpha-tubulin. To identify novel acetylated proteins, we purified several proteins by the affinity to an anti-acetylated-lysine antibody from cell...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1209731
更新日期：2006-11-30 00:00:00
abstract：:We have examined the mitogenic potential of platelet-derived growth factor (PDGF) on AIDS (acquired immune deficiency syndrome)-related and sporadic Kaposi's sarcoma cells in comparison to fibroblasts at physiological and subphysiological calcium concentrations of the culture medium. At low calcium concentrations in t...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:While studying Bim, a BH3-only proapoptotic protein, we identified an approximately 36 kDa protein, which was abundantly expressed in all five strains of primary normal human prostate (NHP) epithelial cells but significantly reduced or lost in seven prostate cancer cell lines. The approximately 36 kDa protein was subs...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1206196
更新日期：2003-03-13 00:00:00
abstract：:Somatostatin receptors (sst1-5) are present in different types of tumors, where they inhibit key cellular processes such as proliferation and invasion. Although ssts are densely expressed in breast cancer, especially sst2, their role and therapeutic potential remain uncertain. Recently, we identified a new truncated s...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2011.389
更新日期：2012-04-19 00:00:00
abstract：:Malignant pleural mesothelioma (MPM) is a highly aggressive tumor that is related to asbestos exposure. MPM is characterized by rapid and diffuse local growth in the thoracic cavity, and it has a poor prognosis because it is often refractory to conventional therapy. Although MPM is an extraordinarily challenging disea...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2009.415
更新日期：2010-02-25 00:00:00
abstract：:Previously, we have mimicked human neurofibromatosis type 2 (NF2) in conditional Nf2 mutant (P0Cre;Nf2flox2/flox2) mice. Schwannomas, characteristic for NF2, were found at low frequency in older mice. Here, we report that these mice, upon additional hemizygosity for p53, rapidly develop multiple tumours showing featur...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1207858
更新日期：2004-08-26 00:00:00
abstract：:Centromeric instability is characterized by dynamic formation of centromeric breaks, deletions, isochromosomes and translocations, which are commonly observed in cancer. So far, however, the mechanisms of centromeric instability in cancer cells are still poorly understood. In this study, we tested the hypothesis that ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2010.508
更新日期：2011-03-17 00:00:00
abstract：:Branching morphogenesis within the peripubertal mouse mammary gland is directed by progesterone (P). A role for the homeobox-containing transcription factor, Msx2, during branching morphogenesis is suggested from its ontogenic expression profile and hormonal regulation. Herein, we define the spatio-temporal control of...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1210555
更新日期：2007-11-29 00:00:00
abstract：:The p53 protein is a multifunctional transcription factor which orchestrates cellular responses to DNA damage, so helping to conserve genomic stability. It may also regulate genes involved in intercellular signalling, such as thrombospondin, a negative regulator of angiogenesis and metastatic spread. Activation of p53...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1995-08-17 00:00:00
abstract：:Chromosome 8 loss of heterozygosity (LOH) in cancer of the urinary bladder is associated with high tumour grade and stage. We have screened 193 cases of transitional cell carcinoma (TCC) of the bladder using 30 microsatellite polymorphisms on chromosome 8. Forty three cases (22%) showed LOH on 8p, the majority of whic...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1996-03-07 00:00:00
abstract：:We have previously reported on the identification of a cDNA (placenta growth factor, PlGF) coding for a novel angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF). Biochemical and functional characterization of PlGF derived from t...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:N-methyl-D-aspartate receptors (NMDARs) are the predominant excitatory neurotransmitter receptors in the mammalian brain. We found that among the three NMDARs examined (NMDAR1, NMDAR2A, NMDAR2B), only NMDAR2A was silenced in colorectal carcinoma (CRC) cell lines at basal line and reactivated by the demethylating agent...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1210842
更新日期：2008-03-27 00:00:00
abstract：:Overexpression of the c-Jun proto-oncogene in MCF7 breast cancer cells results in a variety of phenotype changes related to malignant progression including increased motility and invasion. Concurrent with these phenotypic effects are changes in the expression of multiple gene targets. We previously demonstrated that e...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1205857
更新日期：2002-10-10 00:00:00
abstract：:Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are extracellular lipid mediators that signal through distinct members of the Edg/LP subfamily of G protein-coupled receptors (GPCRs). LPA and S1P receptors are expressed in almost every cell type and can couple to multiple G proteins (G(i), G(q) and G(12/1...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1204187
更新日期：2001-03-26 00:00:00
abstract：:The A-myb gene belongs to the family of the c-myb proto-oncogene. We report here the cloning from a B lymphocyte cDNA library of the previously missing 3' half of the human A-myb cDNA, thus closing the previously still incomplete open reading frame. Analysis of the homologies between the different myb proteins reveals...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:BRCA2 is a breast cancer susceptibility gene. Germline mutations of BRCA2 account for about 10-30% of familial breast cancer cases. Consistent with its tumor-suppressor activity, BRCA2 plays an important role in DNA repair. To assess the susceptibility of carriers of mutant BRCA2 to tumorigenesis induced by DNA-damagi...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1207314
更新日期：2004-03-11 00:00:00
abstract：:An integrative functional genomics study of multiple forms of data are vital for discovering molecular drivers of cancer development and progression. Here, we present an integrated genomic strategy utilizing DNA methylation and transcriptome profile data to discover epigenetically regulated genes implicated in cancer ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2016.176
更新日期：2017-01-05 00:00:00
