Regulation of SV40 large T-antigen stability by reversible acetylation.


:Reversible acetylation on protein lysine residues has been shown to regulate the function of both nuclear proteins such as histones and p53 and cytoplasmic proteins such as alpha-tubulin. To identify novel acetylated proteins, we purified several proteins by the affinity to an anti-acetylated-lysine antibody from cells treated with trichostatin A (TSA). Among the proteins identified, here we report acetylation of the SV40 large T antigen (T-Ag). The acetylation site was determined to be lysine-697, which is located adjacent to the C-terminal Cdc4 phospho-degron (CPD). Overexpression of the CBP acetyltransferase acetylated T-Ag, whereas HDAC1, HDAC3 and SIRT1 bound and deacetylated T-Ag. The acetylation and deacetylation occurred independently of p53, a binding partner of T-Ag, but the acetylation was enhanced in the presence of p53. T-Ag in the cells treated with TSA and NA or the acetylation mimic mutant (K697Q) became unstable in COS-7 cells, suggesting that acetylation regulates stability of T-Ag. Indeed, NIH3T3 cells stably expressing K697Q showed decreased anchorage-independent growth compared with those expressing wild type or the K697R mutant. These results demonstrate that acetylation destabilizes T-Ag and regulates the transforming activity of T-Ag in NIH3T3 cells.






Shimazu T,Komatsu Y,Nakayama KI,Fukazawa H,Horinouchi S,Yoshida M




Has Abstract


2006-11-30 00:00:00
















  • Isolation of chromosome-specific DNA sequences from an Alu polymerase chain reaction library to define the breakpoint in a patient with a constitutional translocation t(1;13) (q22;q12) and ganglioneuroblastoma.

    abstract::We describe the cytogenetic and molecular characterization of a t(1;13)(q22;q12) constitutional rearrangement occurring in a patient with a relatively benign form of neuroblastoma, called ganglioneuroblastoma. Somatic cell hybrids were generated between mouse 3T3 cells and a lymphoblastoid cell line from this patient,...


    pub_type: 杂志文章


    authors: Michalski AJ,Cotter FE,Cowell JK

    更新日期:1992-08-01 00:00:00

  • Inhibition of cyclo-oxygenase 2 expression in colon cells by the chemopreventive agent curcumin involves inhibition of NF-kappaB activation via the NIK/IKK signalling complex.

    abstract::Colorectal cancer is a major cause of cancer deaths in Western countries, but epidemiological data suggest that dietary modification might reduce these by as much as 90%. Cyclo-oxygenase 2 (COX2), an inducible isoform of prostaglandin H synthase, which mediates prostaglandin synthesis during inflammation, and which is...


    pub_type: 杂志文章


    authors: Plummer SM,Holloway KA,Manson MM,Munks RJ,Kaptein A,Farrow S,Howells L

    更新日期:1999-10-28 00:00:00

  • Stat3 promotes the development of erythroleukemia by inducing Pu.1 expression and inhibiting erythroid differentiation.

    abstract::Leukemogenesis requires two classes of mutations, one that promotes proliferation and one that blocks differentiation. The erythroleukemia induced by Friend virus is a multistage disease characterized by an early proliferative stage driven by the interaction of the viral glycoprotein, gp55, with Sf-Stk and the EpoR, a...


    pub_type: 杂志文章


    authors: Hegde S,Ni S,He S,Yoon D,Feng GS,Watowich SS,Paulson RF,Hankey PA

    更新日期:2009-09-24 00:00:00

  • The Tek/Tie2 receptor signals through a novel Dok-related docking protein, Dok-R.

    abstract::Tek/Tie2 is an endothelial cell-specific receptor tyrosine kinase that has been shown to play a role in vascular development of the mouse. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathway(s) mediated by this rec...


    pub_type: 杂志文章


    authors: Jones N,Dumont DJ

    更新日期:1998-09-03 00:00:00

  • Molecular interdiction of Src-family kinase signaling in hematopoietic cells.

    abstract::The ability of Src-family kinases (SFKs) to mediate signaling from cell surface receptors in hematopoietic cells is a function of their catalytic activity, location and binding partners. Kinase activity is regulated in the cell by kinases and phosphatases that alter the state of phosphorylation of key tyrosine residue...


    pub_type: 杂志文章,评审


    authors: Geahlen RL,Handley MD,Harrison ML

    更新日期:2004-10-18 00:00:00

  • Restoring PUMA induction overcomes KRAS-mediated resistance to anti-EGFR antibodies in colorectal cancer.

    abstract::Intrinsic and acquired resistance to anti-EGFR antibody therapy, frequently mediated by a mutant or amplified KRAS oncogene, is a significant challenge in the treatment of colorectal cancer (CRC). However, the mechanism of KRAS-mediated therapeutic resistance is not well understood. In this study, we demonstrate that ...


    pub_type: 杂志文章


    authors: Knickelbein K,Tong J,Chen D,Wang YJ,Misale S,Bardelli A,Yu J,Zhang L

    更新日期:2018-08-01 00:00:00

  • E2F1 suppresses skin carcinogenesis via the ARF-p53 pathway.

    abstract::The E2F1 transcription factor, which is deregulated in most human cancers by mutations in the p16-cyclin D-Rb pathway, has both oncogenic and tumor-suppressive properties. This is dramatically illustrated by the phenotype of an E2F1 transgenic mouse model that spontaneously develops tumors in the skin and other epithe...


    pub_type: 杂志文章


    authors: Russell JL,Weaks RL,Berton TR,Johnson DG

    更新日期:2006-02-09 00:00:00

  • PHLPP2 stabilization by p27 mediates its inhibition of bladder cancer invasion by promoting autophagic degradation of MMP2 protein.

    abstract::Pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2) is a tumor suppressor that catalyzes the de-phosphorylation of the AGC kinases, while p27 acts as a tumor suppressor that regulates cell cycle, apoptosis, and cell motility. Our previous studies have identified that PHLPP2 participates in in...


    pub_type: 杂志文章


    authors: Peng M,Wang J,Zhang D,Jin H,Li J,Wu XR,Huang C

    更新日期:2018-10-01 00:00:00

  • Prothymosin-alpha mRNA expression correlates with that of c-myc in human colon cancer.

    abstract::Prothymosin alpha (PT-alpha) is a nuclear protein involved in cell proliferation. Transcription of PT-alpha has been reported to be regulated by the c-myc gene in vitro. We identified PT-alpha as being overexpressed in a human colon cancer minus normal mucosa subtraction cDNA library. Northern blot (messenger RNA) ana...


    pub_type: 杂志文章


    authors: Mori M,Barnard GF,Staniunas RJ,Jessup JM,Steele GD Jr,Chen LB

    更新日期:1993-10-01 00:00:00

  • P-Rex1 participates in Neuregulin-ErbB signal transduction and its expression correlates with patient outcome in breast cancer.

    abstract::The Neuregulins and their receptors, the ErbB/HER subfamily of receptor tyrosine kinases, have critical roles in animal physiology, and their deregulation is frequent in cancer. Here we report the identification of the guanine nucleotide exchange factor, phosphatidylinositol 3,4,5-triphosphate-dependent Rac exchanger ...


    pub_type: 杂志文章


    authors: Montero JC,Seoane S,Ocaña A,Pandiella A

    更新日期:2011-03-03 00:00:00

  • Modulation of c-myc oncogene expression by phorbol ester and interferon-gamma: appraisal by flow cytometry.

    abstract::A flow cytometric assay was developed to examine the expression of the cellular myc oncogene in relation to cell cycle in individual cells. C-myc-oncoprotein was detected by indirect immunofluorescence using a purified sheep polyclonal antibody, anti-human-myc. Specific binding of anti-human-myc was measured by flow c...


    pub_type: 杂志文章


    authors: Mohamed AN,Nakeff A,Mohammad RM,KuKuruga M,al-Katib A

    更新日期:1988-10-01 00:00:00

  • EGFRvIII gene rearrangement is an early event in glioblastoma tumorigenesis and expression defines a hierarchy modulated by epigenetic mechanisms.

    abstract::Amplification and rearrangements of the epidermal growth factor receptor (EGFR) gene are frequently found in glioblastoma multiforme (GBM). The most common variant is EGFR variant III (EGFRvIII). Research suggests that EGFRvIII could be a marker for a cancer stem cell or tumor-initiating population. If amplification a...


    pub_type: 杂志文章


    authors: Del Vecchio CA,Giacomini CP,Vogel H,Jensen KC,Florio T,Merlo A,Pollack JR,Wong AJ

    更新日期:2013-05-23 00:00:00

  • CKIP-1 acts as a colonic tumor suppressor by repressing oncogenic Smurf1 synthesis and promoting Smurf1 autodegradation.

    abstract::Dysregulation of cellular signaling pathways can lead to colon cancer. However, research on the key signaling effectors or regulators in colon carcinogenesis is limited. Casein kinase-2 interacting protein-1 (CKIP-1; also known as PLEKHO1) is crucial during adult bone formation and is a promising drug target for osteo...


    pub_type: 杂志文章


    authors: Nie J,Liu L,Xing G,Zhang M,Wei R,Guo M,Li X,Xie P,Li L,He F,Han W,Zhang L

    更新日期:2014-07-10 00:00:00

  • Phosphorylation of Ets1 regulates the complementation of a CSF-1 receptor impaired in mitogenesis.

    abstract::Ets1, the founder member of the Ets transcription factor family, is involved in a variety of developmental and cellular processes. Previous studies have shown that serine phosphorylation of Ets1 inhibits its DNA binding activity, suggesting that phosphorylation is important in the regulation of Ets1 function. To furth...


    pub_type: 杂志文章


    authors: Rabault B,Roussel MF,Quang CT,Ghysdael J

    更新日期:1996-08-15 00:00:00

  • In vivo and in vitro effects of v-fos and EJ-Ha-ras oncogene expression in murine epidermal keratinocytes.

    abstract::Primary neonatal Balb/c keratinocyte (NEK) cultures grown, using 3T3 feeder cell support in high calcium, serum supplemented medium, were transfected with EJ-Ha-ras or v-fos DNA sequences and pSV2 neo. Several neo resistant clones were isolated and several established cell lines expressing the transfected gene product...


    pub_type: 杂志文章


    authors: Appleby MW,Greenfield IM,Crook T,Parkinson EK,Stanley MA

    更新日期:1989-11-01 00:00:00

  • Expression of SCC-S2, an antiapoptotic molecule, correlates with enhanced proliferation and tumorigenicity of MDA-MB 435 cells.

    abstract::SCC-S2/GG2-1/NDED is a recently discovered antiapoptotic molecule induced by the activation of the transcription factor NF-kappaB. Here we have examined a role of SCC-S2 in cell growth regulation in vitro and in vivo. Western blotting using an antipeptide antibody revealed endogenous SCC-S2 as a approximately 21 kDa c...


    pub_type: 杂志文章


    authors: Kumar D,Gokhale P,Broustas C,Chakravarty D,Ahmad I,Kasid U

    更新日期:2004-01-15 00:00:00

  • The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression.

    abstract::In this study, we examined the role of the E-cadherin-repressed gene human Nanos1 (hNanos1) in tumor invasion process. First, our in vivo study revealed that hNanos1 mRNAs were overexpressed in invasive lung carcinomas. Moreover, hNanos1 was co-localized with MT1-MMP (membrane type 1-matrix metalloproteinase) in E-cad...


    pub_type: 杂志文章


    authors: Bonnomet A,Polette M,Strumane K,Gilles C,Dalstein V,Kileztky C,Berx G,van Roy F,Birembaut P,Nawrocki-Raby B

    更新日期:2008-06-12 00:00:00

  • Association of RACK1 and PKCbeta with the common beta-chain of the IL-5/IL-3/GM-CSF receptor.

    abstract::Granulocyte macrophage colony stimulating factor (GM-CSF), interleukin-3 (IL-3) and interleukin-5 (IL-5 belong to a family of cytokines that regulate proliferation, differentiation and function of haematopoietic cells. Their receptor consists of a ligand specific alpha-chain and a signal transducing beta-chain (betac)...


    pub_type: 杂志文章


    authors: Geijsen N,Spaargaren M,Raaijmakers JA,Lammers JW,Koenderman L,Coffer PJ

    更新日期:1999-09-09 00:00:00

  • Apoptosis in v-myc transformation of myelomonocytic cells and its modulation by CSF-1.

    abstract::c-myc is a proto-oncogene essential for cell growth. When activated, its expression can lead to uncontrolled cell proliferation, transformation and tumorigenesis. The cell line tEMmyc4 is a murine myelomonocytic cell line that was established following transformation by v-myc. It has a high level of v-myc expression a...


    pub_type: 杂志文章


    authors: Dolnikov A,Ward RL,Hawkins NJ,Symonds G

    更新日期:1996-03-21 00:00:00

  • Death inducer-obliterator 1 (Dido1) is a BMP target gene and promotes BMP-induced melanoma progression.

    abstract::Bone morphogenetic proteins (BMPs) are known to play an important role in melanoma development and progression. However, the downstream targets of BMPs have not been investigated thus far. Therefore, we treated melanoma cell lines with the Smad-specific BMP inhibitor Dorsomorphin and performed a cDNA microarray. We id...


    pub_type: 杂志文章


    authors: Braig S,Bosserhoff AK

    更新日期:2013-02-14 00:00:00

  • Hepatocyte growth factor induces apoptosis through the extrinsic pathway in hepatoma cells: favouring role of hypoxia-inducible factor-1 deficiency.

    abstract::Two hepatocarcinoma cell lines, the Hepa-1 wild-type (c1c7) and the beta-subunit mutated (c4) lacking hypoxia-inducible factor-1 (HIF-1) activity, were differentially susceptible to apoptosis by hepatocyte growth factor (HGF). The c4 cells were 40% apoptotic 48 h after HGF treatment. On the contrary, the wild-type c1c...


    pub_type: 杂志文章


    authors: Matteucci E,Modora S,Simone M,Desiderio MA

    更新日期:2003-06-26 00:00:00

  • SVCT-2 in breast cancer acts as an indicator for L-ascorbate treatment.

    abstract::L-ascorbate (L-ascorbic acid, vitamin C) clearly has an inhibitory effect on cancer cells. However, the mechanism underlying differential sensitivity of cancer cells from same tissue to L-ascorbate is yet to be clarified. Here, we demonstrate that L-ascorbate has a selective killing effect, which is influenced by sodi...


    pub_type: 杂志文章


    authors: Hong SW,Lee SH,Moon JH,Hwang JJ,Kim DE,Ko E,Kim HS,Cho IJ,Kang JS,Kim DJ,Kim JE,Shin JS,Jung DJ,Jeong YJ,Cho BJ,Kim TW,Lee JS,Kang JS,Hwang YI,Noh DY,Jin DH,Lee WJ

    更新日期:2013-03-21 00:00:00

  • Synthetic lethality of Chk1 inhibition combined with p53 and/or p21 loss during a DNA damage response in normal and tumor cells.

    abstract::Cell cycle checkpoints ensure genome integrity and are frequently compromised in human cancers. A therapeutic strategy being explored takes advantage of checkpoint defects in p53-deficient tumors in order to sensitize them to DNA-damaging agents by eliminating Chk1-mediated checkpoint responses. Using mouse models, we...


    pub_type: 杂志文章


    authors: Origanti S,Cai SR,Munir AZ,White LS,Piwnica-Worms H

    更新日期:2013-01-31 00:00:00

  • The nuclear BAG-1 isoform, BAG-1L, enhances oestrogen-dependent transcription.

    abstract::BAG-1 is a multifunctional protein that interacts with a wide range of cellular targets including heat-shock proteins and some nuclear hormone receptors. BAG-1 exists as three major isoforms, BAG-1L, BAG-1M and BAG-1S. BAG-1L contains a nuclear localization signal, which is not present in the other isoforms, and is pr...


    pub_type: 杂志文章


    authors: Cutress RI,Townsend PA,Sharp A,Maison A,Wood L,Lee R,Brimmell M,Mullee MA,Johnson PW,Royle GT,Bateman AC,Packham G

    更新日期:2003-08-07 00:00:00

  • Serine/threonine phosphatases in the DNA damage response and cancer.

    abstract::The cellular response to DNA damage is a crucial surveillance mechanism that maintains genomic integrity and prevents cancer progression. Previous studies identified multiple Ser/Thr protein kinases that have pivotal roles in the activation of this response. It is interesting that a growing body of evidence suggests t...


    pub_type: 杂志文章,评审


    authors: Peng A,Maller JL

    更新日期:2010-11-11 00:00:00

  • The 'WS motif' common to v-mpl and members of the cytokine receptor superfamily is dispensable for myeloproliferative leukemia virus pathogenicity.

    abstract::Several motifs are conserved in the extracellular domain of the cloned chains of the recently described cytokine receptor superfamily. One of them, usually close to the transmembrane region, is the 'WS motif'. Its function remains unknown, but it has been recently shown that the integrity of this motif is essential fo...


    pub_type: 杂志文章


    authors: Bénit L,Charon M,Cocault L,Wendling F,Gisselbrecht S

    更新日期:1993-03-01 00:00:00

  • Frequent hypermethylation of the RASSF1A gene in prostate cancer.

    abstract::Recently, we have cloned and characterized the Ras association domain family 1A gene (RASSF1A) at 3p21.3, from which loss of genetic material is one of the most frequent events in several types of human solid tumors. The CpG island promoter region of this gene is highly methylated in several human cancers, most notabl...


    pub_type: 杂志文章


    authors: Liu L,Yoon JH,Dammann R,Pfeifer GP

    更新日期:2002-10-03 00:00:00

  • Modulation of cellular apoptotic potential: contributions to oncogenesis.

    abstract::The importance of apoptosis as a natural means to eliminate unwanted or damaged cells has been realized over the past decade. Many components required to exercise programmed cell death have been identified and shown to pre-exist in most, if not all, cells. Such ubiquity requires that apoptosis be tightly controlled an...


    pub_type: 杂志文章,评审


    authors: Stambolic V,Mak TW,Woodgett JR

    更新日期:1999-11-01 00:00:00

  • Latent membrane protein 1 of Epstein-Barr virus coordinately regulates proliferation with control of apoptosis.

    abstract::Latent membrane protein 1 (LMP1), an oncoprotein encoded by Epstein-Barr virus (EBV), is an integral membrane protein, which acts like a constitutively active receptor. LMP1 is critical for some facet of EBV's induction and maintenance of proliferation of infected B cells. It, in part, mimics signaling by the CD40 rec...


    pub_type: 杂志文章


    authors: Dirmeier U,Hoffmann R,Kilger E,Schultheiss U,Briseño C,Gires O,Kieser A,Eick D,Sugden B,Hammerschmidt W

    更新日期:2005-03-03 00:00:00

  • Oncogenic transformation by beta-catenin: deletion analysis and characterization of selected target genes.

    abstract::Genetic analysis of beta-catenin-induced oncogenic transformation in chicken embryo fibroblasts (CEF) revealed the following prerequisites for oncogenicity: (1) removal of the N terminal phosphorylation sites targeted by glycogen synthase kinase 3beta (GSK3beta), (2) retention of the N terminal transactivation domain,...


    pub_type: 杂志文章


    authors: Aoki M,Sobek V,Maslyar DJ,Hecht A,Vogt PK

    更新日期:2002-10-10 00:00:00