Abstract:
:Leptin, a recently identified hormone, is believed to reduce appetite and maintain body weight. The mRNA of leptin is expressed only in mature adipose cells. To clarify the regulation of leptin gene expression in adipocytes, 3T3-L1 adipocytes were treated for 16 h with various agents known to modulate lipid metabolism, and then the leptin mRNA was measured by the reverse transcription-polymerase chain reaction method. Interestingly, both norepinephrine and isoproterenol reduced the level of leptin mRNA to about 20% of that found in untreated control cells in a dose-dependent fashion. The maximum reduction occurred at 100 nmol/l of either norepinephrine or isoproterenol, and the half-maximal effect was observed at approximately 3 nmol/l norepinephrine and approximately 1 nmol/l isoproterenol. Propranolol reversed about 50% of the reduction by either norepinephrine or isoproterenol. In contrast, phentolamine did not inhibit the reduction by either norepinephrine or isoproterenol. Moreover, both cholera toxin and dibutyryl cAMP decreased the level of leptin mRNA to about 10% of that in control cells in a dose-dependent fashion. The maximum effect was elicited at 100 ng/ml cholera toxin and 100 micromol/l dibutyryl cAMP. The concentration producing the half-maximal effect was approximately 1 ng/ml cholera toxin and approximately 50 micromol/l dibutyryl cAMP. Dibutyryl cGMP, however, did not affect leptin gene expression. These results suggest that a signaling pathway that results in the activation of protein kinase A regulates leptin gene expression in 3T3-L1 adipocytes.
journal_name
Diabetesjournal_title
Diabetesauthors
Kosaki A,Yamada K,Kuzuya Hdoi
10.2337/diab.45.12.1744subject
Has Abstractpub_date
1996-12-01 00:00:00pages
1744-9issue
12eissn
0012-1797issn
1939-327Xjournal_volume
45pub_type
杂志文章相关文献
DIABETES文献大全abstract::Improvement of glycemic status by insulin is associated with profound changes in amino acid metabolism in type 1 diabetes. In contrast, a dissociation of insulin effect on glucose and amino acid metabolism has been reported in type 2 diabetes. Type 2 diabetic patients are reported to have reduced muscle oxidative enzy...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.51.8.2395
更新日期:2002-08-01 00:00:00
abstract::To clarify the event that is involved in the pathogenesis of impaired glucose tolerance (IGT), we studied 15 individuals with IGT and 15 subjects with normal tolerance using the minimal model approach. Our IGT subjects were characterized by normal insulin secretory responses to oral glucose and mild impairments in ins...
journal_title:Diabetes
pub_type: 临床试验,杂志文章
doi:10.2337/diab.43.10.1211
更新日期:1994-10-01 00:00:00
abstract:OBJECTIVE:The ATP-binding cassette transporter A1 (ABCA1) R230C variant is associated with low HDL cholesterol levels, obesity, and the metabolic syndrome in Mexican-Mestizos. Because a pivotal role for ABCA1 in pancreatic beta-cell function was recently observed in the mouse model, we assessed the association of this ...
journal_title:Diabetes
pub_type: 杂志文章,多中心研究
doi:10.2337/db07-0484
更新日期:2008-02-01 00:00:00
abstract::We previously reported a syndrome of severe hyperinsulinemia and early-onset hypertension in three patients with dominant-negative mutations in the nuclear hormone receptor peroxisome proliferator-activated receptor (PPAR)-gamma. We now report the results of further detailed pathophysiological evaluation of these subj...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.4.910
更新日期:2003-04-01 00:00:00
abstract::Prolonged near-physiological pulsatile insulin infusion has a greater hypoglycemic effect than continuous insulin infusion. We have previously shown that continuous hyperinsulinemia induces insulin insensitivity. This study examines the mechanisms responsible for the greater hypoglycemic effect of pulsatile insulin ad...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.39.4.501
更新日期:1990-04-01 00:00:00
abstract::Diabetes results from an inadequate mass of functional beta-cells. Such inadequacy could result from loss of beta-cells due to an immune assault or the inability to compensate for insulin resistance. Thus, mechanisms that regulate the number of beta-cells will be key to understanding both the pathogenesis of diabetes ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0249
更新日期:2006-11-01 00:00:00
abstract::The effect of nonenzymatic glycosylation on the biologic function of human antithrombin III was evaluated using a chromogenic thrombin substrate assay in the presence of catalytic amounts of heparin. Experimental conditions that increased the rate of nonenzymatic protein glycosylation were associated with decreases in...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.6.532
更新日期:1984-06-01 00:00:00
abstract::Members of the tumor necrosis factor receptor superfamily play an important role in the initiation, expansion, and termination of an immune response. It has recently been demonstrated that one member of this family, CD30, plays a central role in maintaining peripheral tolerance by controlling the expansion of autoreac...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.9.1612
更新日期:2000-09-01 00:00:00
abstract:OBJECTIVE:Sarco-endoplasmic reticulum Ca(2+)-ATPase 2b (SERCA2b) and SERCA3 pump Ca(2+) in the endoplasmic reticulum (ER) of pancreatic β-cells. We studied their role in the control of the free ER Ca(2+) concentration ([Ca(2+)](ER)) and the role of SERCA3 in the control of insulin secretion and ER stress. RESEARCH DES...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-1543
更新日期:2011-10-01 00:00:00
abstract::We examined the effects of aldose reductase inhibition (ARI) on glomerular filtration rate (GFR), albuminuria, and kidney histology in partially insulin-treated streptozocin-induced diabetic (STZ-D) rats. After 1 mo of diabetes, GFR was elevated over control values in the STZ-D rats but was not affected by treatment w...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.8.981
更新日期:1989-08-01 00:00:00
abstract::Islet β-cells adapt to insulin resistance through increased insulin secretion and expansion. Type 2 diabetes typically occurs when prolonged insulin resistance exceeds the adaptive capacity of β-cells. Our prior screening efforts led to the discovery that adenosine kinase (ADK) inhibitors stimulate β-cell replication....
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-0816
更新日期:2017-07-01 00:00:00
abstract::It is accepted for insulin-secreting cells in culture that the closure of ATP-sensitive K+ channels causes the glucose-dependent depolarization of pancreatic beta-cells seen at subthreshold levels (less than 100 mg/dl) of glucose. The question remains for the more thoroughly studied beta-cells in freshly dissected int...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.4.416
更新日期:1989-04-01 00:00:00
abstract::African Americans are at increased risk of type 2 diabetes and many diabetes complications. We have carried out a genome-wide scan for African American type 2 diabetes using 638 affected sibling pairs (ASPs) from 247 families ascertained through impaired renal function to identify type 2 diabetes loci in this high-ris...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.3.830
更新日期:2004-03-01 00:00:00
abstract::The past several years have seen an explosive increase in our understanding of the transcriptional basis of adipose cell differentiation. In particular, a key role has been illustrated for PPAR-gamma, a member of the nuclear hormone receptor superfamily. PPAR-gamma has also been recently identified as the major functi...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.47.4.507
更新日期:1998-04-01 00:00:00
abstract::Insulin receptors from rat hepatoma cells (Fao) and human placenta were partially purified by detergent solubilization and lectin purification. The insulin receptor preparations were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis under reducing or nonreducing conditions. The proteins were...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.10.923
更新日期:1984-10-01 00:00:00
abstract::The molecular and cellular level reaches of the metabolic dysregulations that characterize diabetes are yet to be fully discovered. As mechanisms underlying management of reactive oxygen species (ROS) gain interest as crucial factors in cell integrity, questions arise about the role of redox cues in the regulation and...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db18-0232
更新日期:2019-01-01 00:00:00
abstract::GAD autoantibodies (GADAs) are sensitive markers of islet autoimmunity and type 1 diabetes. They form the basis of robust prediction models and are widely used for the recruitment of subjects at high risk of type 1 diabetes to prevention trials. However, GADAs are also found in many individuals at low risk of diabetes...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db14-1693
更新日期:2015-09-01 00:00:00
abstract::Insulin replacement therapy in type 1 diabetes is imperfect because proper glycemic control is not always achieved. Most patients develop microvascular, macrovascular, and neurological complications, which increase with the degree of hyperglycemia. Engineered muscle cells continuously secreting basal levels of insulin...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.51.3.704
更新日期:2002-03-01 00:00:00
abstract::Considerable debate exists about whether alterations in mitochondrial respiratory capacity and/or content play a causal role in the development of insulin resistance during obesity. The current study was undertaken to determine whether such alterations are present during the initial stages of insulin resistance in hum...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-0940
更新日期:2014-01-01 00:00:00
abstract::Class IIa histone deacetylases (HDACs), such as HDAC4, HDAC5, and HDAC7, provide critical mechanisms for regulating glucose homeostasis. Here we report that HDAC9, another class IIa HDAC, regulates hepatic gluconeogenesis via deacetylation of a Forkhead box O (FoxO) family transcription factor, FoxO1, together with HD...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db15-0197
更新日期:2015-12-01 00:00:00
abstract::Insulin secretion from the pancreas is pulsatile. The precise site and function of the pacemaker that regulates insulin periodicity in humans have not been determined. We isolated human pancreatic islets from five cadaver organ donors by collagenase digestion and density gradient purification. After 24 h of culture in...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.6.827
更新日期:1994-06-01 00:00:00
abstract:OBJECTIVE:To evaluate whether healthy or diabetic adult mice can tolerate an extreme loss of pancreatic α-cells and how this sudden massive depletion affects β-cell function and blood glucose homeostasis. RESEARCH DESIGN AND METHODS:We generated a new transgenic model allowing near-total α-cell removal specifically in...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-0876
更新日期:2011-11-01 00:00:00
abstract::Recent data have suggested a key role for tumor necrosis factor (TNF)-alpha in the insulin resistance of obesity and non-insulin-dependent diabetes mellitus (NIDDM). TNF-alpha expression is elevated in the adipose tissue of multiple experimental models of obesity. Neutralization of TNF-alpha in one of these models imp...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diab.43.11.1271
更新日期:1994-11-01 00:00:00
abstract::Diet-induced obesity is known to cause peripheral insulin resistance in rodents. We have recently found that feeding cod protein to high-fat-fed rats prevents the development of insulin resistance in skeletal muscle. In the present study, we have further explored the cellular mechanisms behind this beneficial effect o...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.1.29
更新日期:2003-01-01 00:00:00
abstract::To evaluate possible advantages of human insulin of recombinant DNA origin (HI) in the treatment of diabetic patients, we compared cellular and humoral immunoreactivities of HI and porcine insulin (PI). Anti-insulin IgE bound equal amounts of 125I-HI and 125I-PI. There was no difference between HI- and PI-stimulated l...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.6.516
更新日期:1983-06-01 00:00:00
abstract::We investigated whether the effect of troglitazone on glucose disposal is associated with altered insulin signaling. Nondiabetic first-degree relatives of type 2 diabetic patients (age 30 +/- 2 years, BMI 30 +/- 1 kg/m(2); n = 20) were randomized in a double-blind manner to 3 months of troglitazone (200 mg/day) or pla...
journal_title:Diabetes
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2337/diabetes.51.9.2691
更新日期:2002-09-01 00:00:00
abstract::Duct-occluded segmental-pancreas transplants develop progressive fibrotic atrophy, even though endocrine function seems to be unaffected. To determine end-stage size of the graft and to evaluate magnetic resonance imaging (MRI) and computed tomography (CT), these imaging techniques were applied in eight patients with ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.1.s24
更新日期:1989-01-01 00:00:00
abstract::The aim of this study was to test the hypothesis that plasma leptin concentrations contributed to the pathophysiology of NIDDM by decreasing both insulin-mediated glucose disposal and glucose-stimulated insulin secretion. The study was performed in 60 women with normal oral glucose tolerance. Differences in insulin-me...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.47.2.244
更新日期:1998-02-01 00:00:00
abstract::We evaluated the production of antibodies against insulin in a genetically well-defined population. In the first study, 124 young patients with type I diabetes for longer than 6 mo were included. Anti-insulin antibodies were detected by polyethyleneglycol (PEG) precipitation after incubation of acidified, charcoal-str...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.35.6.634
更新日期:1986-06-01 00:00:00
abstract::Plasma and urine fibrinopeptide-A (FPA) levels were investigated in type I and II diabetic patients. Plasma FPA and 24-h urinary excretion of FPA were significantly elevated in diabetic patients compared with normal volunteers, indicating augmented thrombin activity in diabetes. Plasma and urine FPA did not differ bet...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.34.9.836
更新日期:1985-09-01 00:00:00