Abstract:
:Diet-induced obesity is known to cause peripheral insulin resistance in rodents. We have recently found that feeding cod protein to high-fat-fed rats prevents the development of insulin resistance in skeletal muscle. In the present study, we have further explored the cellular mechanisms behind this beneficial effect of cod protein on skeletal muscle insulin sensitivity. Rats were fed a standard chow diet or a high-fat diet in which the protein source was either casein, soy, or cod proteins for 4 weeks. Whole-body and muscle glucose disposal were reduced by approximately 50% in rats fed high-fat diets with casein or soy proteins, but these impairments were not observed in animals fed cod protein. Insulin-induced tyrosine phosphorylation of the insulin receptor and insulin receptor substrate (IRS) proteins were similar in muscle of chow- and high-fat-fed rats regardless of the dietary protein source. However, IRS-1-associated phosphatidylinositol (PI) 3-kinase activity was severely impaired (-60%) in muscle of high-fat-fed rats consuming casein or soy protein. In marked contrast, feeding rats with cod protein completely prevented the deleterious effect of fat feeding on insulin-stimulated PI 3-kinase activity. The activation of the downstream kinase Akt/PKB by insulin, assessed by in vitro kinase assay and phosphorylation of GSK-3beta, were also impaired in muscle of high-fat-fed rats consuming casein or soy protein, but these defects were also fully prevented by dietary cod protein. However, no effect of cod protein was observed on atypical protein kinase C activity. Normalization of PI 3-kinase/Akt activation by insulin in rats fed high-fat diets with cod protein was associated with improved translocation of GLUT4 to the T-tubules but not to the plasma membrane. Taken together, these results show that dietary cod protein is a natural insulin-sensitizing agent that appears to prevent obesity-linked muscle insulin resistance by normalizing insulin activation of the PI 3-kinase/Akt pathway and by selectively improving GLUT4 translocation to the T-tubules.
journal_name
Diabetesjournal_title
Diabetesauthors
Tremblay F,Lavigne C,Jacques H,Marette Adoi
10.2337/diabetes.52.1.29subject
Has Abstractpub_date
2003-01-01 00:00:00pages
29-37issue
1eissn
0012-1797issn
1939-327Xjournal_volume
52pub_type
杂志文章相关文献
DIABETES文献大全abstract::Ambulatory blood pressure (AMBP) measurements were obtained at 20-min intervals for 24 h in 25 subjects with insulin-dependent (type I) diabetes mellitus and 21 control subjects. The diabetic patients had normal kidney function (glomerular filtration rate 112.1 +/- 7.2 ml.min-1.1.73 m-2, renal plasma flow 459.0 +/- 23...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.39.12.1556
更新日期:1990-12-01 00:00:00
abstract:OBJECTIVE:Rosiglitazone (RSG) is an insulin-sensitizing drug used to treat type 2 diabetes mellitus. The A Diabetes Outcome Progression Trial (ADOPT) shows that women taking RSG experienced more fractures than patients taking other type 2 diabetes drugs. These were not osteoporotic vertebral fractures but, rather, occu...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-1672
更新日期:2011-12-01 00:00:00
abstract::Chronic hyperglycemia desensitizes beta-cells to glucose. To further define the mechanisms behind desensitization and the role of overstimulation, we tested human pancreatic islets for the effects of long-term elevated glucose levels on cytoplasmic free Ca2+ concentration ([Ca2+]i) and its relationship to overstimulat...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.11.1840
更新日期:2000-11-01 00:00:00
abstract::To explore metabolic changes associated with the sorbitol accumulation and myo-inositol depletion observed in glomeruli of rats with experimental diabetes, we examined total and ouabain-inhibited adenosine triphosphatase (ATPase) activity in glomeruli isolated from control and streptozocin (STZ)-diabetic rats. Glomeru...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.34.11.1071
更新日期:1985-11-01 00:00:00
abstract::The association between clinical autonomic dysfunction and myocardial MIBG accumulation was investigated. The study groups comprised 6 male diabetic patients with autonomic neuropathy (ANP+ group), 6 male diabetic patients without autonomic neuropathy (ANP-group), and 6 male nondiabetic control subjects. The mean age ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.41.9.1069
更新日期:1992-09-01 00:00:00
abstract::Marked improvement in glycemic control occurs in patients with type 2 diabetes mellitus shortly after Roux-en-Y gastric bypass surgery (RYGB) and before there is major weight loss. The objective of this study was to determine whether the magnitude of this change is primarily due to caloric restriction or is unique to ...
journal_title:Diabetes
pub_type: 临床试验,杂志文章
doi:10.2337/db12-1762
更新日期:2013-09-01 00:00:00
abstract::Rapid and sustained stimulation of beta-cells with glucose induces biphasic insulin secretion. The two phases appear to reflect a characteristic of stimulus-secretion coupling in each beta-cell rather than heterogeneity in the time-course of the response between beta-cells or islets. There is no evidence indicating th...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.51.2007.s60
更新日期:2002-02-01 00:00:00
abstract::Immunosuppressive drugs are routinely used to provide tolerance after whole pancreas and islet cell transplantations. While they are essential in inhibiting graft rejection, little is known about their effect on islet function and beta-cell viability. In this study, we report that tacrolimus, sirolimus, and mycophenol...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.54.2.424
更新日期:2005-02-01 00:00:00
abstract::Production of reactive oxygen species (ROS) is a key instigator of β-cell dysfunction in diabetes. The pleiotropic cytokine interleukin 6 (IL-6) has previously been linked to β-cell autophagy but has not been studied in the context of β-cell antioxidant response. We used a combination of animal models of diabetes and ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db17-1280
更新日期:2018-08-01 00:00:00
abstract::Genome-wide nonparametric linkage analysis of 480 sib-pairs affected with type 2 diabetes revealed linkage to a previously unreported susceptibility locus on chromosome 18p11. This result improved with stringent subphenotyping using age- and sex-adjusted BMI, ultimately reaching a logarithm of odds of 3.82 (allele sha...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.3.675
更新日期:2001-03-01 00:00:00
abstract::Previous studies have indicated that individuals with type 2 diabetes have an increased resting metabolic rate (RMR) but decreased insulin-induced thermogenesis (IIT) compared with those with normal glucose tolerance (NGT). When and by which mechanisms these abnormalities occur during the development of diabetes remai...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.48.8.1607
更新日期:1999-08-01 00:00:00
abstract:OBJECTIVE:Chronic pancreatitis, characterized by pancreatic exocrine tissue destruction with initial maintenance of islets, eventually leads to insulin-dependent diabetes in most patients. Mice deficient for the transcription factors E2F1 and E2F2 suffer from a chronic pancreatitis-like syndrome and become diabetic. Su...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-1577
更新日期:2008-06-01 00:00:00
abstract::The uptake of 2-deoxy-D-[1-14C]-glucose, in the presence and absence of insulin, was measured in adipocytes from normal and streptozotocin-diabetic animals, over a wide temperature range (25-45 degrees C). Optimum temperatures for uptake in the presence of maximally stimulating insulin concentrations occurred near phy...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.3.258
更新日期:1984-03-01 00:00:00
abstract::Acute insulin secretion determines the efficiency of glucose clearance. Moreover, impaired acute insulin release is characteristic of reduced glucose control in the prediabetic state. Incretin hormones, which increase β-cell cAMP, restore acute-phase insulin secretion and improve glucose control. To determine the phys...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-1013
更新日期:2013-05-01 00:00:00
abstract::Exosomes are important for intercellular communication, but the role of exosomes in the communication between adipose tissue (AT) and the liver remains unknown. The aim of this study is to determine the contribution of AT-derived exosomes in nonalcoholic fatty liver disease (NAFLD). Exosome components, liver fat conte...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db20-0146
更新日期:2021-02-01 00:00:00
abstract::Two sisters presented with severe insulin resistance and markedly decreased insulin binding to erythrocytes, cultured fibroblasts, and transformed lymphocytes. The dose-response curve of insulin-stimulated amino acid uptake in the fibroblasts was shifted to the right. The molecular weight of the insulin receptor on th...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.5.653
更新日期:1988-05-01 00:00:00
abstract::The ability to transfer immunoregulatory, cytoprotective, or antiapoptotic genes into pancreatic islet cells may allow enhanced posttransplantation survival of islet allografts and inhibition of recurrent autoimmune destruction of these cells in type 1 diabetes. However, transient transgene expression and the tendency...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.3.515
更新日期:2001-03-01 00:00:00
abstract::Diabetes-induced alterations in heme and hemoproteins, as well as its relationship to drug-mediated induction of ALA Synthase (ALA-S), were examined in female Sprague-Dawley rats. Animals were rendered diabetic by a single i.v. injection of streptozotocin (STZ, 65 mg/kg) and measurements were made at various times aft...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.1.37
更新日期:1984-01-01 00:00:00
abstract::Leptin resistance has recently been confirmed not only in animal obese models but in human obesity. Evidence is rapidly emerging that suggests that activation of histamine signaling in the hypothalamus may have substantial anti-obesity and antidiabetic actions, particularly in leptin-resistant states. To address this ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.2.376
更新日期:2001-02-01 00:00:00
abstract::To examine the impact of diabetes and its treatment on plasma free-fatty acid (FFA) and oxidative fuel metabolism during hypoglycemia, we combined indirect calorimetry with [3-3H]glucose during a 4-h low-dose insulin infusion (plasma insulin approximately 2-fold above basal) in six poorly controlled and nine well-cont...
journal_title:Diabetes
pub_type: 临床试验,杂志文章
doi:10.2337/diab.39.2.134
更新日期:1990-02-01 00:00:00
abstract::To investigate the short-term effects of didecanoyl-L-alpha-phosphatidylcholine on the nasal mucosa and the mechanism by which didecanoyl-L-alpha-phosphatidylcholine enhances the nasal absorption of insulin, an in vitro model was developed. The mucosa from the posterior part of the rabbit nasal septum was mounted in a...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.42.7.1032
更新日期:1993-07-01 00:00:00
abstract::Defects in insulin production and signaling are suspected to share a key role in diabetes and Alzheimer disease (AD), two age-related pathologies. In this study, we investigated the interrelation between AD and diabetes using a high-fat diet (HFD) in a mouse model of genetically induced AD-like neuropathology (3xTg-AD...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db14-0375
更新日期:2014-12-01 00:00:00
abstract::For the period of 1 January 1979 through 31 December 1985, an average annual incidence rate (IR) of 12.1/100,000 (95% confidence interval [10.4/100,000, 14.0/100,000]) of insulin-dependent diabetes mellitus (IDDM) was observed in Jefferson County, Alabama, among people less than 20 yr old. Hospital medical-record revi...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.5.629
更新日期:1989-05-01 00:00:00
abstract::In skeletal muscle, the actin cytoskeleton-regulating GTPase, Rac1, is necessary for insulin-dependent GLUT4 translocation. Muscle contraction increases glucose transport and represents an alternative signaling pathway to insulin. Whether Rac1 is activated by muscle contraction and regulates contraction-induced glucos...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-0491
更新日期:2013-04-01 00:00:00
abstract::Activation of the sympathetic nervous system (SNS) constitutes a putative mechanism of obesity-induced insulin resistance. Thus, we hypothesized that inhibiting the SNS by using renal denervation (RDN) will improve insulin sensitivity (SI) in a nonhypertensive obese canine model. SI was measured using euglycemic-hyper...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-0698
更新日期:2016-11-01 00:00:00
abstract::Insulin resistance is an underappreciated facet of type 1 diabetes that occurs with remarkable consistency and considerable magnitude. Although therapeutic innovations are continuing to normalize dysglycemia, a sizable body of data suggests a second metabolic abnormality-iatrogenic hyperinsulinemia-principally drives ...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/dbi19-0026
更新日期:2020-05-01 00:00:00
abstract::In diabetic subjects, polyol pathway activity might inhibit neutrophil function and cause nerve damage. The effects of ponalrestat, an aldose reductase inhibitor, were assessed on neutrophil intracellular killing of Escherichia coli and on autonomic function in diabetic subjects in a randomized double-blind, placebo-c...
journal_title:Diabetes
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2337/diab.42.2.336
更新日期:1993-02-01 00:00:00
abstract::This article seeks to determine whether factors related to autoimmunity risk remain significant after the initiation of two or more diabetes-related autoantibodies and continue to contribute to type 1 diabetes (T1D) risk among autoantibody-positive children in The Environmental Determinants of Diabetes in the Young (T...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db17-0261
更新日期:2017-12-01 00:00:00
abstract::We addressed the question of whether newly diagnosed type I (insulin-dependent) diabetes mellitus patients showed an increased number of DR (la+) T-lymphocytes compared with nondiabetic siblings and normal control subjects. Two-color flow cytometry measurements of peripheral-blood lymphocytes showed a slight but stati...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.6.792
更新日期:1988-06-01 00:00:00
abstract::The last two decades have witnessed an explosion of interest in adipocyte biology, coinciding with the upsurge of obesity and metabolic syndrome. Now we have new perspectives on the distinct developmental origins of white, brown, and beige adipocytes and their role in metabolic physiology and disease. Beyond fuel meta...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/dbi18-0009
更新日期:2019-05-01 00:00:00