Abstract:
:Recent pharmacological studies have shown that perivascular nerves can influence the development and function of vascular endothelial cells (ECs). However, morphological studies have not yet been carried out to investigate whether these functional changes are associated with changes in vasoactive substances in ECs. We used postembedding electron microscopy (EM) triple gold-labeling immunocytochemistry to study the effects of short-term sympathectomy (3 days after 6-hydroxydopamine [6-OHDA] treatment) and long-term sympathectomy (guanethidine and 8 days after 6-OHDA) on the distribution of vasoactive substances in ECs of the rat thoracic aorta. The post-embedding immunocytochemistry, which can detect levels of label in individual cells, showed that there was a significant decrease in endothelial NO synthase (NOS3)-labeled, serotonin (5-HT)-labeled, and substance P (SP)-labeled, but a significant increase in endothelin-1 (ET-1)-labeled, gold particles in ECs after long-term, but not after short-term (3-day), sympathectomy. In conclusion, our results show that long-term sympathectomy causes an increase in ET-1 and decrease in NOS3, 5-HT, and SP immunoreactivity in ECs of the thoracic aorta. Our data also indicate that postembedding EM triple gold-labeling immunocytochemistry is a valuable technique for quantitative studies of the content of vasoactive substances in ECs.
journal_name
Circ Resjournal_title
Circulation researchauthors
Aliev G,Ralevic V,Burnstock Gdoi
10.1161/01.res.79.2.317subject
Has Abstractpub_date
1996-08-01 00:00:00pages
317-23issue
2eissn
0009-7330issn
1524-4571journal_volume
79pub_type
杂志文章abstract::Whole-cell voltage-clamp experiments were performed on enzymatically dissociated single ventricular myocytes harvested from feline endocardial and epicardial surfaces. The studies were designed to test the hypothesis that the differences in the amplitude of transient outward current (Ito) contribute to the difference ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.67.5.1287
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abstract::The role of Ca(2+)-dependent potassium channels in mediating vascular responses to activation of adenylate cyclase in vivo is not known. The goal of this study was to examine the hypothesis that dilatation of cerebral arterioles in response to activation of adenylate cyclase is mediated by activation of Ca(2+)-depende...
journal_title:Circulation research
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2018-12-07 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.88.1.30
更新日期:2001-01-19 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.86.2.233
更新日期:2000-02-04 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.82.8.908
更新日期:1998-05-04 00:00:00
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journal_title:Circulation research
pub_type: 新闻
doi:10.1161/RES.0b013e3181ffeac0
更新日期:2010-10-29 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.78.4.627
更新日期:1996-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.59.1.43
更新日期:1986-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.51.5.614
更新日期:1982-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.5.957
更新日期:1995-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.0000032490.04207.bd
更新日期:2002-09-06 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.65.1.63
更新日期:1989-07-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.62.2.315
更新日期:1988-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.64.6.1190
更新日期:1989-06-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.110.233403
更新日期:2012-01-20 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000261352.81736.37
更新日期:2007-03-30 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.88.4.422
更新日期:2001-03-02 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.117.311450
更新日期:2017-12-08 00:00:00
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pub_type: 杂志文章
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更新日期:1976-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-07-01 00:00:00
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doi:10.1161/01.res.68.2.319
更新日期:1991-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2009-07-17 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:1975-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-09-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.62.5.1001
更新日期:1988-05-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.6.1166
更新日期:1995-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.60.4.626
更新日期:1987-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.78.4.724
更新日期:1996-04-01 00:00:00