Abstract:
:A new protocol has been developed for selective removal of the endothelium from the arteries of the perfused rat hind-limb preparation. The hind limb was perfused with oxygenated Krebs-Ringer bicarbonate solution with a combination of high flow and timed air bubbles (2 minutes at high flow followed by 5 minutes of air bubbles, followed by a further 2 minutes at high flow). Representative vessels at different diameters of the arterial tree--the femoral artery, the artery supplying the extensor hallucis proprius muscle, and the arteries, arterioles, and capillaries within the hallucis proprius muscle--were taken for examination by transmission and scanning electron microscopy. A graded degree of damage occurred along the length of the hind-limb vasculature, with endothelial cells having been completely removed from the femoral artery, removed from or severely damaged in the artery supplying the hallucis proprius muscle, and partially damaged in arteries within the hallucis proprius muscle. However, no damage occurred to the endothelial cells of the arterioles and capillaries within the hallucis proprius muscle. The integrity of the vascular bed after endothelial removal according to the above protocol was confirmed by the demonstration of no diminution, but in fact an increase, in contractile responses to bolus injections of alpha,beta-methylene ATP, resulting in a shift to the left of the dose-response curve. The viability was further confirmed by the fact that the conductance (flow/perfusion pressure) of the preparation during the periods of high flow was no different before and after removal of the endothelium.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Circ Resjournal_title
Circulation researchauthors
Ralevic V,Kristek F,Hudlická O,Burnstock Gdoi
10.1161/01.res.64.6.1190subject
Has Abstractpub_date
1989-06-01 00:00:00pages
1190-6issue
6eissn
0009-7330issn
1524-4571journal_volume
64pub_type
杂志文章abstract::Purkinje cells contain sarcoplasmic reticulum (SR) directly under the surface membrane, are devoid of t-tubuli, and are packed with myofibrils surrounded by central SR. Several studies have reported that electrical excitation induces a biphasic Ca(2+) transient in Purkinje fiber bundles. We determined the nature of th...
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abstract::The introduction of statins ≈ 30 years ago ushered in the era of lipid lowering as the most effective way to reduce risk of atherosclerotic cardiovascular disease. Nonetheless, residual risk remains high, and statin intolerance is frequently encountered in clinical practice. After a long dry period, the field of thera...
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更新日期:1987-06-01 00:00:00
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更新日期:1992-03-01 00:00:00
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更新日期:1989-12-01 00:00:00
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更新日期:2020-02-28 00:00:00
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更新日期:2007-08-31 00:00:00
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更新日期:1990-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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更新日期:1984-09-01 00:00:00
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更新日期:2008-08-29 00:00:00
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更新日期:2011-07-08 00:00:00
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doi:10.1161/01.res.51.1.43
更新日期:1982-07-01 00:00:00
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更新日期:1997-03-01 00:00:00
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更新日期:2015-04-10 00:00:00
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更新日期:2018-12-07 00:00:00
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更新日期:1984-09-01 00:00:00
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更新日期:2019-01-18 00:00:00
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更新日期:1992-07-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
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更新日期:1994-03-01 00:00:00