Abstract：
:D-penicillamine (PSH) is a copper chelator that generates hydrogen peroxide and inhibits neovascularization. As hydrogen peroxide is toxic to some tumor cells and to blood vessels, we reasoned that PSH plus copper would inhibit tumors in vivo and in vitro. To test this hypothesis, we first incubated murine J558L plasmacytoma cells with varying combinations of drug (PSH and/or copper sulfate) plus modulators (fetal calf serum, dithiothrietol, catalase, eosinophil peroxidase) and then used fluorescence microscopy to measure cell proliferation, necrosis, and apoptosis. We also incubated various types of human tumor cells with PSH plus copper for 24 hours and then measured the number of surviving cells 24 hours later. For the in vivo studies, we measured the effects of 7 daily i.p. injections of 10 mg of PSH on the growth rates of interleukin-5 genetransfected J558L tumors in 20 BALB/c mice. Our experiments demonstrated that PSH plus copper exerted a significant antiproliferative effect on tumor cells in vitro that was neutralized by protein or catalase and enhanced by adherent eosinophil peroxidase. Human acute myelogenous leukemia cells were especially sensitive to PSH plus copper. In vivo, however, PSH had no significant effect on the growth rates of J558L tumors that were infiltrated by eosinophils. We conclude that the interaction of PSH-copper with tumors is primarily antiproliferative, mediated by hydrogen peroxide, and inhibitable by protein. Therefore, for PSH to be an effective antineoplastic drug strategies will need to be developed to prevent its rapid neutralization by protein.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Samoszuk M,Nguyen Vsubject
Has Abstractpub_date
1996-05-01 00:00:00pages
1219-23issue
3Aeissn
0250-7005issn
1791-7530journal_volume
16pub_type
杂志文章abstract：:The triphenylmethane derivative, aurintricarboxylic acid (ATA), but not aurin, is a potent inhibitor of angiogenesis in the chick chorioallantoic membrane. ATA alone showed potent anti-angiogenic activity in a dose-related manner. The presence of heparin or bFGF decreased the anti-angiogenic activity of ATA. ATA also ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Osteosarcoma of the jaw is a rare disease; we report two cases, one in which the primary osteosarcoma had occurred in the sacrum and ileum, the second at the mandible. Dissemination of osteosarcoma to other organs, especially early dissemination to the lung, is common, but metastasis to the jaw has only rarely been re...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：BACKGROUND:The doxorubicin-docetaxel combination is active in breast cancer; the aim of the present study was to evaluate the complete response rate and safety profile of the doxorubicin and docetaxel regimen as first-line chemotherapy in metastatic breast cancer patients. PATIENTS AND METHODS:Forty-three patients ent...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy (PDT) following topical application of 5-aminolevulinic acid (ALA) is increasingly employed for several types of malignancies. However, data with respect to tissue penetration and distribution of ALA-induced porphyrins after topical application are scarce. Therefore, it was our aim to st...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Taxane-based chemotherapy is useful for peritoneal dissemination control in advanced/recurrent gastric cancer; however, insufficient relative dose intensity (RDI) may preclude disease control achievement. Nab-paclitaxel, with high tumour permeability, is a promising second- or later-line treatment. PATIENTS...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11622
更新日期：2017-05-01 00:00:00
abstract：:Small cell lung cancer is known to carry a high capacity to metastasize in various sites including bone marrow. We here review the respective sensitivities of classical cyto-histological methods and of more recently described techniques such as cell cultures, immunostaining with monoclonal antibodies and magnetic reso...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1994-11-01 00:00:00
abstract：:Normal human skin fibroblasts and cells from 2 benign (3T3 and 3T6B) and 1 malignant (3T6D) fibroblast lines were grown in the presence of C20 fatty acids in the concentration range 0-100 mg/l culture medium. The culture medium consisted of 10% FCS and 90% DMEM, the cells were dosed with various concentrations of fatt...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND/AIM:Inflammation is a key process in gastric carcinogenesis. Cytokines are mediators of inflammation and are involved in metastasis and tumorigenicity. We previously assessed the role of cytokine gene polymorphisms in gastric cancer risk in Chile. In the present study, we aimed to analyze whether these polym...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12907
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:Chemotherapy for collecting duct carcinoma (CDC) has demonstrated only limited efficacy in the advanced setting. The present study evaluated the activity of targeted therapies in metastatic CDC. PATIENTS AND METHODS:We evaluated a cohort of 384 consecutive patients with metastatic renal cell carcinoma (mRCC...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
abstract：:We have previously shown that oral administration of PSK not only exerts antitumor activity, but also restores immune response in tumor-bearing mice. In order to analyze the immunomodulative effect of PSK, we investigated the effects of oral PSK on the intestinal immune systems in ICR mice bearing sarcoma 180. Oral ad...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:Currently, feeder cells are γ-irradiated immediately before use for the ex vivo expansion of natural killer (NK) cells from human peripheral blood. Storing irradiated feeder cells by cryopreserving them in multiple vials would be more convenient than irradiating cells each time they are needed. We compared NK cell exp...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
abstract：AIM:To determine the predictive value of HIF-1α and VEGF-C in primary neuroendocrine breast cancers (NEBC) and their correlation with other clinico-pathological characteristics of NEBC. MATERIALS AND METHODS:HIF-1α and VEGF-C were determined immunohistochemically in tissue samples from 31 cases of NEBC. RESULTS:The H...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：:Serially propagated fibroblast clones were treated with 25-mM methionine and a thioguanine resistance assay was performed at each passage to measure mutagenesis at the HGPRT locus. The spontaneous frequency of thioguanine resistant mutants before treatment was less than 5 X 10(-6) in four clones and 22 X 10(-6) in one...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：BACKGROUND/AIM:The European MRI and Rectal Cancer Surgery (EuMaRCS) score was proposed to identify preoperatively difficult laparoscopic total mesorectal excision (L-TME) for locally advanced rectal cancer (LARC). This study aimed to test EuMaRCS's validity. PATIENTS AND METHODS:Data were retrieved from a European mul...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14166
更新日期：2020-04-01 00:00:00
abstract：:The insufficient interobserver reproducibility limits the practical use of histomorphological tumor grading in daily routine pathology. In this study, the reasons for the quite low rates of agreement between different observers have been investigated by the application of a factor analytical technique, i.e. principal ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：BACKGROUND/AIM:4H-1-Benzopyran-4-ones (chromones) have provided backbone structure for the chemical synthesis of potent anticancer drugs. In this study, the cytotoxicity of fifteen 2-(N-cyclicamino)chromone derivatives was investigated and subjected to quantitative structure-activity relationship (QSAR) analysis. MATE...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12674
更新日期：2018-07-01 00:00:00
abstract：:AIDS-related Epstein-Barr virus (EBV)-associated T cell lymphomas are emerging as a new, distinct histopathological entity. The pathway whereby EBV infects T cells as well as the initial EBV transcriptional program in T cells has not been established. In order to shed light on the early events of the EBV infection of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Three histamine H2-receptor antagonists, Cimetidine, Metiamide and Ranitidine, were tested for their inhibitory effect on two experimental bowel cancer models. In the first model mitotic rates were measured in dimethylhydrazine-induced tumors of rat colon and in the second model volumetric changes in human large bowel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Human epidemiological studies suggest an association between N-acetyltransferase (NAT) activity and the incidence of bladder and colorectal cancers. In this study, paclitaxel was selected to examine the inhibition of arylamine NAT activity, gene expression and 2-aminofluorene-DNA adduct formation in a human osteogenic...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：UNLABELLED:The present study evaluated the frequency of the polymorphism of Interleukin-6 (IL6) in women positive for E6/E7 Human Papillomavirus (HPV) (n=152) and women negative for HPV (n=238), 390 women in total. Material for analysis was obtained at the Federal University of São Paulo. Interleukin-6 polymorphism was...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：:Surgery remains the mainstay of treatment for breast cancer, including complete or partial mastectomy and lumpectomy. Breast reconstruction has gained popularity mainly due to its tremendous impact on the psychological status of the patients. Autologous fat grafting is a well-established method used in cosmetic surger...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.12112
更新日期：2017-12-01 00:00:00
abstract：AIM:To elucidate the behavior of 1 phosphatidylinositol 4,5 bisphosphate (PIP2) 5 phosphatase (PIP2 5-Pase, EC 3.1.3.36) and 1 phosphatidylinositol 4 phosphate (PIP) 4-phosphatase (PIP 4-Pase) in cancer cells, the steady state activities of PIP2 5-Pase and PIP 4-Pase were determined in the particulate fractions of rat ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:We investigated whether the levels of the DNA-dependent protein kinase (DNA-PK) activity and content correlate with drug sensitivity in different tumour materials and if this can be utilised in predicting treatment outcome. DNA-PK activity and expression were investigated in tumour cells from 8 patients with chronic l...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:Evidence has been presented that tumor suppressor genes p53 and Rb play a crucial role in the development of both human prostate and bladder cancer. Patients with either cancer are at an increased risk for developing the other malignancy as compared to the general population. The purpose of the present study was to in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:The use of gene-modified dendritic cells (DC) is a powerful tool to enhance antitumor immune responses stimulated by these cells in cancer immunotherapy. Cationized gelatin is preferably incorporated via phagocytosis and is gradually degraded by proteolysis while buffering lysosomal activity. This may be appropriate f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：:The usefulness of lymphocyte subset change as an indicator for predicting survival time and the effectiveness of combined treatment with an immunopotentiator, lentinan, and carboplatin (CBDCA) were investigated. Of 13 patients with advanced unresectable cancer entered in this study, 9 were administered 2 mg/body lenti...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：BACKGROUND:Low 5-fluorouracil (5-FU) concentrations cause a significant increase in DNA synthesis in mitogen-activated human lymphocytes. MATERIALS AND METHODS:We explored 2.5 microM 5-FU-induced DNA synthesis by testing 5-FU activity in hypoxanthine-aminopterin-thymidine (HAT)-containing medium, and its effect on thy...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-10-01 00:00:00
abstract：BACKGROUND:Recent studies have demonstrated that heparin-binding epidermal growth factor-like growth factor (HB-EGF) plays a role in carcinogenesis and carcinoma progression. In this study we investigated the expression of HB-EGF in human colonic non-neoplastic and neoplastic tissues. MATERIALS AND METHODS:We performe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:A series of 2-triazenoindole derivatives was prepared in moderate to good yields by coupling 2-diazoindoles with secondary amines. Only 3-ethoxycarbonyl-2-(3.3-tetramethylenetriazenyl)-indole, tested against a panel of 60 human tumor cell lines, showed moderate activity on breast, ovarian and CNS cancer with GI50 in t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:The search for promising modulators of cancer multidrug resistance (MDR) that are able to reduce the activity of P-glycoprotein, thus restoring the cytotoxicity of anticancer drugs, is ongoing. The identification of compounds that overcome the apoptosis deficiency that frequently accompanies MDR is also of g...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
