Abstract:
:Because the administration of hematopoietic growth factors and the use of stem cell support often fails to alleviate the neutropenic phase induced by cytotoxic drugs, several investigators have attempted to expand ex vivo hematopoietic progenitors for clinical use. These attempts have clearly shown that the cultured cells are functional and can be safely administered to patients, but that the in vivo performance is disappointing and the concept as a whole is not yet clinically useful. The major reasons for these unsuccessful attempts are thought to be cumbersome cell fractionation techniques, contamination, prolonged incubation, and the use of less than ideal cytokine combinations. In response, we have developed a simple procedure for ex vivo expansion of myeloid progenitor cells. In this assay, unfractionated mononuclear cells from apheresis donors are incubated in nonpyrogenic plastic bags for 7 days in the presence of culture medium either containing fetal calf serum or human plasma, granulocyte colony-stimulating factor, and stem cell factor. We have demonstrated that under these conditions the number of colony-forming units (CFU) granulocyte-macrophage (CFU-GM) and of CFU-granulocyte-macrophage-erythroid-megakaryocyte (CFU-GEMM) increased 7- and 9-fold, respectively, by day 7 and the number of burst-forming units-erythroid (BFU-E) increased 2.7-fold by day 5 of culture. Significant increases in the numbers of cells expressing CD34+, CD34+/CD38+, CD34+/CD33+, CD34+/CD15+, and CD34+/CD90+ and significant declines in the numbers of cells expressing CD34+/CD38- and CD19 surface antigens were also observed. The relative numbers of cells expressing T-cell markers and CD56 surface antigen did not change. By using different concentrations of various hematopoietic growth factor combinations, we can increase the number of mature and immature cells of different hematopoietic lineages.
journal_name
J Clin Apherjournal_title
Journal of clinical apheresisauthors
Estrov Z,Huh YO,Ginsberg CF,Harris D,Van Q,Mirza NQ,Talpaz M,Korbling Mdoi
10.1002/jca.10012subject
Has Abstractpub_date
2002-01-01 00:00:00pages
7-16issue
1eissn
0733-2459issn
1098-1101pii
10.1002/jca.10012journal_volume
17pub_type
杂志文章abstract::The influence of 6 weeks of cyclosporin A (CYA), followed by a further 6 weeks of this agent in combination with plasma exchange (PE), was defined on panel reactivity and titre of preformed cytotoxic antibodies in stable patients on haemodialysis awaiting renal transplantation. Nine individuals with antibodies to 30% ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920060107
更新日期:1991-01-01 00:00:00
abstract::The literature on the use of therapeutic apheresis is often criticized for the preponderance of low to very low quality evidence. Shehata et al. examined the apheresis literature published between 1976 and 1999 and found a lack of randomized controlled trials with those identified demonstrating significant limitations...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/jca.21263
更新日期:2013-02-01 00:00:00
abstract::This study examined the incidence and predictors of peripheral blood stem cell (PBSC) mobilization failure in patients with multiple myeloma (MM). Retrospective data for 104 patients who received granulocyte colony-stimulating factor (G-CSF) alone or with cyclophosphamide as mobilization regimens were analyzed. The ra...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21326
更新日期:2014-12-01 00:00:00
abstract::Mobilization regimens for CD34+ cells have generally been judged successful based on the number of cells collected without evaluating mobilization separately from collection. Using retrospective data for patients who collected CD34+ cells on Total Therapy protocols 3a/3b (VTD-PACE) and Total Therapy 4/5 using a novel ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21313
更新日期:2014-10-01 00:00:00
abstract::Plasma replacement for thrombotic thrombocytopenic purpura (TTP) is accomplished with various plasma products. This study sought to determine the incidence of allergic reactions with FFP or CPP as replacement in therapeutic plasma exchange (TPE). Forty-one TTP patients were identified retrospectively who received TPE ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.1025
更新日期:2001-01-01 00:00:00
abstract::Treatment by low density lipoprotein (LDL) apheresis using dextran sulfate columns (DSC) leads to hemostasis alterations with prolonged activated partial thromboplastin time (APTT) of more than 120 seconds. In order to explain this hypocoagulability, we studied hemostasis parameters both in patients and in the extraco...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920080302
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:Effects of therapeutic plasma exchange (TPE) on immune cells and their cytokine production in MS, are unknown. Since interleukine-6 and tumor growth factor-β have critical roles in MS immunopathogenesis, the impacts of TPE on the expression of these cytokines and their receptors on the surface of CD4+ T lymph...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21755
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:Securing adequate vascular access is essential for a successful apheresis procedure. In most, peripheral access is preferred but it is not always technically possible. Ultrasound-Guided Peripheral Vascular Access (USG-PIVA) is a well-documented technique in the setting of Emergency departments. However, limi...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21440
更新日期:2016-12-01 00:00:00
abstract::We describe the case of a 24 year old male with severe fistulating Crohn's disease who failed mutliple modalities of medical and surgical therapy. We present objective and subjective evidence of disease remission after induction and maintenance treatment with leukocyte apheresis. This is the first described case of su...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21308
更新日期:2014-06-01 00:00:00
abstract::Leukemic-dendritic cells (leukemic-DCs) have certain limitations, which include difficult generation in 30-40% of patients, and low levels of expression of several key molecules. Therefore, an alternative approach using monocyte-derived DCs pulsed with tumor antigens is required. We investigated the possibility of imm...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20085
更新日期:2006-10-01 00:00:00
abstract::Transfusion-acquired babesiosis can be an asymptomatic or self-limited febrile hemolytic illness in a healthy host. A persistent, relapsing, and/or fulminant course with the development of life-threatening complications may be seen in immunocompromised or splenectomized patients. As in malaria, erythrocyte parasitemia...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20262
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Nephrogenic systemic fibrosis (NSF), also known as nephrogenic sclerosing dermopathy (NSD), is a rare progressive fibrosing disease associated with gadolinium based dyes in patients with renal disease. The exact pathophysiology is not well understood. Accepted treatments include corticosteroids, immune modul...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21253
更新日期:2013-08-01 00:00:00
abstract::The recent introduction of low-density lipoprotein (LDL)/very low-density lipoprotein (VLDL) selective-removal systems offers an alternative to plasma exchange (PE). For the last 10 years, we have treated a male homozygous hypercholesterolemia type IIA patient with PE using 5% normal serum albumin (NSA) replacement, P...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920070206
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Estimation of the total blood volume (TBV) is fundamental to the control of automated red cell exchange (RCE). Error in the TBV estimate can produce outcomes that deviate from the prescribed targets, and may endanger the patient. Both the Spectra Optia and the COBE Spectra use the Nadler formu...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21748
更新日期:2019-12-01 00:00:00
abstract::Modest success has been achieved with the use of high-dose cytotoxic therapy and bone marrow transplantation in solid tumors. Patient outcome can potentially be improved with further intensification of the therapy. The rapid hematologic recovery achieved with mobilized peripheral blood progenitor cells (PBPC) may redu...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920090307
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Transfusion of D+ red blood cells (RBCs) to D- recipients can be accidental or necessary due to D- RBC shortage. Alloimmunization can complicate future transfusions; implications for women of childbearing age are compounded by possible hemolytic disease of the fetus and newborn. Rh immunoprophylaxis is effec...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20225
更新日期:2010-01-01 00:00:00
abstract::This study chronicles leukocyte- and erythrocyte-depleted platelet concentrate collection by a dual stage channel in which three variables: 1) donor peripheral blood platelet concentration, 2) total blood processed, and 3) collection volume were statistically correlated with platelet yield as determined by a multiple ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920020304
更新日期:1985-01-01 00:00:00
abstract::Excess free iodide in the blood (ingested or injected) may cause thyrotoxicosis in patients at risk. Iodinated contrast solutions contain small amounts of free iodide and may be of significance for patients affected by Graves' disease, multinodular goiter or living in areas of iodine deficiency. Herein, we report a 57...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20161
更新日期:2008-01-01 00:00:00
abstract::The infusion of high-dose (275 mg/kg body weight) immune globulin intravenous (IGIV) after 466 plasma exchanges in 64 patients with autoimmune disease was studied. Side effects occurred during 15% of IGIV infusions. For the most part they were transient and mild, and could be controlled by slowing the infusion rate. T...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920010207
更新日期:1983-01-01 00:00:00
abstract::During the course of heparin-induced extracorporeal lipoprotein apheresis, a patient with no prior known renal impairment or proteinuria demonstrated sustained improvement in estimated glomerular filtration rate, commensurate with reduction in serum lipids and creatine phosphokinase levels. Causes and implications of ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20272
更新日期:2011-01-01 00:00:00
abstract::Thrombopoietin (c-Mpl ligand) is the hematopoietic growth factor that is responsible for regulating the production of platelets from bone marrow megakaryocytes. This approximately 90 kd protein has recently been isolated and is comprised of an erythropoietin domain that is approximately 50% homologous to erythropoieti...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1098-1101(1996)11:3<149::AID-JCA6>3.
更新日期:1996-01-01 00:00:00
abstract::Extracorporeal photopheresis (ECP) has been shown to be clinically effective in the treatment of many T cell-mediated conditions. ECP's mechanism of action includes the induction of apoptosis and the release of pro-inflammatory cytokines. Recently, we have observed early lymphoid apoptosis, detectable immediately post...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.10039
更新日期:2002-01-01 00:00:00
abstract::Peripheral blood stem cells (PBSC) reinfusion appears to hasten hematologic reconstitution following myeloablative therapy. While procurement of PBSC adds apheresis procedures, rapid engraftment could decrease the demand for platelet transfusions. To determine the impact of PBSC collection on workload in our apheresis...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920070408
更新日期:1992-01-01 00:00:00
abstract:INTRODUCTION:ABO- or HLA-incompatible kidney transplantation is possible thanks to pretransplant antibody-depletion achieved by extracorporeal-treatment modalities. These methods induce depletion of some plasma proteins and may also impact on proteins involved in hemostasis. METHODS:To determine the impact of one sess...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21825
更新日期:2020-08-17 00:00:00
abstract::Acute thrombotic thrombocytopenic purpura (TTP) occurred in three patients following abdominal surgeries. One patient underwent extensive lysis for intestinal adhesions with bowel resection, another cholecystectomy for acute cholecystitis, and the third right colectomy and partial intestinal resection for colon cancer...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/1098-1101(2000)15:3<176::aid-jca4>3.0.co;2
更新日期:2000-01-01 00:00:00
abstract::Management of aTTP in patients who refuse or are intolerant to plasma remains challenging, but new drugs can be implemented with success. A 39-year-old woman presented to the Emergency department for bruises at the upper and lower limbs together with worsening anemia and thrombocytopenia; PLASMIC score was seven, indi...
journal_title:Journal of clinical apheresis
pub_type:
doi:10.1002/jca.21877
更新日期:2021-01-18 00:00:00
abstract::Lipid-apheresis (LA) is thought to improve microcirculation. However, limited data are available on the effects on peripheral microcirculation. We investigated upper limb microcirculation of 22 patients undergoing regular LA on a weekly basis before and after LA. Using standardized semiquantitative scales, we analyzed...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20285
更新日期:2011-01-01 00:00:00
abstract::Therapeutic plasma exchange (TPE), a form of extracorporeal therapy, has been used in the management of many systemic conditions some of which include the systemic vasculitides that affect the kidneys. The following is a mini review of what is known about renal related indications for TPE in such conditions and the ev...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/jca.20306
更新日期:2011-01-01 00:00:00
abstract::Extracorporeal photopheresis (ECP) in young pediatric patients has a risk for procedural hypotension and anemia due to extracorporeal fluid shifts. A standard mitigation policy in these patients is to prime the device with packed red blood cells (RBC) or whole blood. We now report multiple episodes of hemolysis while ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21740
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Factors affecting progenitor cell mobilization in patients with non-Hodgkin's lymphoma (NHL) and Hodgkin's lymphoma (HL) are incompletely understood. The aim of this retrospective study was to determine which factors are crucial for effective mobilization and collection of autologous peripheral blood stem ce...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21204
更新日期:2012-01-01 00:00:00