Abstract:
:Extracorporeal photopheresis (ECP) has been shown to be clinically effective in the treatment of many T cell-mediated conditions. ECP's mechanism of action includes the induction of apoptosis and the release of pro-inflammatory cytokines. Recently, we have observed early lymphoid apoptosis, detectable immediately post ECP. We were interested to determine what influence ECP has on pro-inflammatory cytokine secretion at this early pre-infusion stage. Samples from 6 cutaneous T cell lymphoma (CTCL) and 5 graft versus host disease (GvHD) patients were taken pre ECP and immediately post ECP, prior to re-infusion. Following separation, the PBMCs were added to a cell culture medium and stimulated with PMA, Ionomycin, and Brefeldin A for 6 hours. Using flow cytometry, intracellular cytokine expression of IFNgamma and TNFalpha was determined in the T cell population. The monocytes were evaluated for IL6, IFNgamma, IL12, and TNFalpha. For both patient groups, the number of IFNgamma-expressing T cells fell significantly at re-infusion, whilst both T cell- and monocyte-expressing TNFalpha levels were reduced at re-infusion. All other cytokines tested showed no significant change post ECP. For GvHD, pro-inflammatory cytokines have a pathological role. Their down-regulation may have a direct clinical benefit. However, the reduction in the number of IFNgamma- and TNFalpha-expressing mononuclear cells means, at this early stage, it is unlikely that these cytokines assist in the removal of the malignant Th2 cells present in CTCL.
journal_name
J Clin Apherjournal_title
Journal of clinical apheresisauthors
Bladon J,Taylor Pdoi
10.1002/jca.10039subject
Has Abstractpub_date
2002-01-01 00:00:00pages
177-82issue
4eissn
0733-2459issn
1098-1101journal_volume
17pub_type
杂志文章abstract::Thrombopoietin (c-Mpl ligand) is the hematopoietic growth factor that is responsible for regulating the production of platelets from bone marrow megakaryocytes. This approximately 90 kd protein has recently been isolated and is comprised of an erythropoietin domain that is approximately 50% homologous to erythropoieti...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1098-1101(1996)11:3<149::AID-JCA6>3.
更新日期:1996-01-01 00:00:00
abstract::Many practitioners believe in the phenomenon of being labeled either a "black cloud" or "white cloud" while on-call. A "white-cloud" physician is usually defined as one who sees fewer cases while a "black-cloud" is one who often receives more cases. To evaluate these phenomena, a 35-month prospective study was designe...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21650
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Specialized centers claim a need for blood component production independent from the general blood transfusion services. We performed a prospective change control analysis of the transfer of platelet (PLT) production for hematological patients at the University Hospital Basel from the Departme...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21214
更新日期:2012-01-01 00:00:00
abstract::Until recently, the collection of peripheral blood progenitor cells (PBPC) has been semi-automated by using the COBE Spectra, with the operator manually maintaining the position of the white cells being collected. The COBE Spectra Version 6.0 apheresis device offers the user an automated program for the collection of ...
journal_title:Journal of clinical apheresis
pub_type: 临床试验,杂志文章
doi:10.1002/(sici)1098-1101(1999)14:1<26::aid-jca5>3.0
更新日期:1999-01-01 00:00:00
abstract::Apheresis procedures in small children are technically challenging and require special planning with attention to extracorporeal volume. Discontinuous procedures such as extracorporeal photopheresis (ECP) require additional consideration. Alternative methods to perform ECP have been utilized in small children that req...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20231
更新日期:2010-01-01 00:00:00
abstract::The issues of providing quality blood products and maintaining donor safety are primary aims of blood transfusion services. A comprehensive quality system should be in place to fulfill these aims, which can be attained through strict adherence to the established standard operating procedures (SOPs). The Drugs and Cosm...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20056
更新日期:2005-07-01 00:00:00
abstract::Graphite furnace atomic absorption spectroscopy was used to demonstrate a fivefold increase in the median plasma aluminium levels of four patients undergoing therapeutic plasmapheresis. This plasma aluminium loading resulted from contamination of albumin incorporated in the replacement fluids and took place during its...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920030306
更新日期:1987-01-01 00:00:00
abstract::Many apheresis units lack the resources to acquire customized computer software for record keeping. We have adapted a commercially available "spreadsheet" program (Lotus 1-2-3) to aid in quality control activities for plateletpheresis. Data are entered in a grid pattern wherein each donation occupies one row and succe...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920050407
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Transfusion of D+ red blood cells (RBCs) to D- recipients can be accidental or necessary due to D- RBC shortage. Alloimmunization can complicate future transfusions; implications for women of childbearing age are compounded by possible hemolytic disease of the fetus and newborn. Rh immunoprophylaxis is effec...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20225
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND/AIMS:High-volume plasma exchange (HVPE), defined as an exchange of 8 to 12 L per day per procedure or 15% of the ideal body weight with fresh frozen plasma, has shown promising results in improving the survival of patients with acute liver failure (ALF). However, clinical evidence is limited. The aim of this...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21873
更新日期:2021-01-05 00:00:00
abstract::Therapeutic plasma exchange (TPE) is an increasingly utilized immunosuppressive adjunct for treatment of antibody-mediated rejection (AMR) following organ transplantation. TPE works through removal of donor-specific HLA antibodies (DSAs) in the recipient's plasma. However, there is no clear laboratory measure evaluati...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21270
更新日期:2013-08-01 00:00:00
abstract::Lipoprotein apheresis is applied to deplete low density lipoprotein and other apolipoprotein B containing lipoproteins in patients with severe familial hypercholesterolemia, hypertriglyceridemia associated pancreatitis, or lipoprotein (a)-hyperlipoproteinemia. Anticoagulation of the extracorporeal circuit may influenc...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21519
更新日期:2017-12-01 00:00:00
abstract::In the United States, therapeutic plasma exchange (TPE) is both performed and requested by a wide range of services, often on an empiric basis (before a diagnosis is established). Whether empiric therapy is beneficial has not been established. Patients were identified from an electronic procedure log that included tho...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.20059
更新日期:2005-12-01 00:00:00
abstract::Bone marrow (BM) damage after previous chemotherapy, such as that involving alkylating agents, and radiation therapy alone cannot explain poor hematopoietic progenitor cell mobilization. We examined the T lymphocytes of BM in 67 autologous peripheral blood stem cell transplant (auto-PBSCT) patients with non-Hodgkin ly...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21817
更新日期:2020-07-28 00:00:00
abstract:BACKGROUND:Dysphagia screening is oftentimes a focus of hospitalized patients, but dysphagia can also occur in outpatient settings. Dysphagia can be overlooked by nurses and clinicians, and it is therefore important to educate nurses on the importance of dysphagia screenings. METHODS:This was a randomized prospective ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,随机对照试验
doi:10.1002/jca.21572
更新日期:2018-02-01 00:00:00
abstract::Plerixafor in combination with granulocyte-colony stimulating factor (G-CSF) is approved for autologous stem cell mobilization in poor mobilizing patients with multiple myeloma or malignant lymphoma. The purpose of this study was to evaluate efficacy and safety of plerixafor in an immediate rescue approach, administra...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21496
更新日期:2017-08-01 00:00:00
abstract::Because the administration of hematopoietic growth factors and the use of stem cell support often fails to alleviate the neutropenic phase induced by cytotoxic drugs, several investigators have attempted to expand ex vivo hematopoietic progenitors for clinical use. These attempts have clearly shown that the cultured c...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.10012
更新日期:2002-01-01 00:00:00
abstract::The combined use of immunoadsorption (IA) and membrane filtration (MF) may markedly enhance removal of IgM and complement component C1q, supporting its use as an element of recipient desensitization in antibody-incompatible transplantation. However, coagulation factor removal may contribute to altered hemostasis, posi...
journal_title:Journal of clinical apheresis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/jca.21399
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:Effects of therapeutic plasma exchange (TPE) on immune cells and their cytokine production in MS, are unknown. Since interleukine-6 and tumor growth factor-β have critical roles in MS immunopathogenesis, the impacts of TPE on the expression of these cytokines and their receptors on the surface of CD4+ T lymph...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21755
更新日期:2020-04-01 00:00:00
abstract::Over the past 37 year the role of plasma exchange in the treatment of patients with renal disease has undergone several changes. The majority of the changes for the use of plasma exchange relied on randomized control trials and delineations of mechanisms that potentially would benefit from the use of plasma exchange. ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/jca.21221
更新日期:2012-01-01 00:00:00
abstract::For many years dextran sulfate adsorption (DSA) treatment of separated plasma has been an established technology for low-density lipoprotein (LDL)-elimination. Recently a system for the treatment of whole blood based on DSA was introduced into clinical practice. To further characterize DSA treatment of whole blood, th...
journal_title:Journal of clinical apheresis
pub_type: 临床试验,杂志文章
doi:10.1002/jca.20135
更新日期:2007-01-01 00:00:00
abstract::"Leukocyte-depleted" platelet concentrates (LD-SDPC) were prepared by cotton-wool and by polyester filtration of "leukocyte-poor" PC collected by the elutriation technique. The storage characteristics of LD-SDPC were comparable to those of the filtered pools of multiple donor platelet concentrates (LD-MDPC). However, ...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920060304
更新日期:1991-01-01 00:00:00
abstract::Modest success has been achieved with the use of high-dose cytotoxic therapy and bone marrow transplantation in solid tumors. Patient outcome can potentially be improved with further intensification of the therapy. The rapid hematologic recovery achieved with mobilized peripheral blood progenitor cells (PBPC) may redu...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920090307
更新日期:1994-01-01 00:00:00
abstract::Sickle cell disease (SCD) patients are prone to develop complications that include stroke, acute chest syndrome, and other crises. Some of these complications require chronic transfusion therapy or red cell exchange (RCE), either for therapeutic or prophylactic reasons. Due to a discrepancy of red cell antigens betwee...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/jca.20112
更新日期:2006-12-01 00:00:00
abstract::Blood component donations by apheresis have become more common in modern blood transfusion practices. We compared three apheresis instruments (Fenwal Amicus, Fresenius COM.TEC, and Trima Accel) with regard to platelet (PLT) yield, collection efficiency (CE), and collection rate (CR). The single-needle or double-needle...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,随机对照试验
doi:10.1002/jca.21358
更新日期:2015-06-01 00:00:00
abstract::The cell separator MCS-3P is an apheresis system offering the flexibility to collect standardized red blood cells, plasma, and/or platelets from one donor. Two different programs were used for the red cell apheresis--RBCP (collection of one unit of red cells and two units of plasma) and RBCPS (one unit of red cells an...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920090308
更新日期:1994-01-01 00:00:00
abstract::Blood group incompatibility remains a significant barrier to kidney transplantation. Approximately, one-third of donors are blood group incompatible with their intended recipient. Options for these donor-recipient pairs include blood group incompatible transplantation or kidney paired donation. However, the optimal pr...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.21390
更新日期:2015-12-01 00:00:00
abstract::Targeted extracorporeal granulocyte and monocyte apheresis (GMA) has produced clinical efficacy together with down modulation of specific inflammatory cytokines in patients with ulcerative colitis (UC). This study was to investigate if preoperative GMA produces immunological effect on dysregulated immune activity afte...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,随机对照试验
doi:10.1002/jca.21299
更新日期:2014-04-01 00:00:00
abstract::We present clinical data on two boys with chronic relapsing polyneuropathy. Their recurrent episodes of weakness had produced marked disability which was unresponsive to continuous prednisone therapy. Plasmapheresis produced dramatic improvement in muscle strength and functional ability. The remissions induced have be...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章
doi:10.1002/jca.2920010110
更新日期:1982-01-01 00:00:00
abstract::The process of publishing original work in a peer review journal is not complete at the point where the manuscript is first submitted. The journal editors will submit the manuscript to peer review whereby outside experts are asked to vet the manuscript for scientific merit, originality and quality. Reviewers' comments...
journal_title:Journal of clinical apheresis
pub_type: 杂志文章,评审
doi:10.1002/jca.21822
更新日期:2020-08-07 00:00:00