Dendritic cell-based proliferative assays of peripheral T cell responses to tetanus toxoid.

Abstract:

:Antigen-specific proliferative responses of peripheral blood T cells are widely used in humans to study the T cell compartment. However, responses to autoantigens are often weak and poorly reproducible. Here we show, using a test recall antigen (tetanus toxoid), that absolute levels of proliferation, minimally detectable antigen doses, and/or ability to detect statistically significant responses can be enhanced using in vitro-generated autologous dendritic cells as antigen presenting cells.

journal_name

Ann N Y Acad Sci

authors

Narendran P,Elsegood K,Leech NJ,Dayan CM

doi

10.1111/j.1749-6632.2002.tb02963.x

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

170-4

eissn

0077-8923

issn

1749-6632

journal_volume

958

pub_type

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