Abstract：
:The aim of this study was to assess the molecular basis of renal Na,K-ATPase disturbances in response to NO-deficient hypertension induced in rats by NO-synthase inhibition with 40 mg/kg/day N(G)-nitro-L-arginine methyl ester (L-NAME) for four weeks. After 4-week administration of L-NAME, the systolic blood pressure (SBP) increased by 30 %. Three weeks after terminating the treatment, SBP recovered to control value. When activating the Na,K-ATPase with its substrate ATP, a 36 % increase in K(m) and 29 % decrease in V(max) values were observed in NO-deficient rats. During activation with Na+, the V(max) was decreased by 20 % and the K(Na) was increased by 111 %, indicating a profound decrease in the affinity of the Na+-binding site in NO-deficient rats. After spontaneous recovery from hypertension, the V(max) remained at the level as in hypertension for both types of enzyme activation. However, in the presence of lower concentrations of substrate which are of physiological relevance an improvement of the enzyme activity was observed as documented by return of K(m) for ATP to control value. The K(Na) value for Na+ was decreased by 27 % as compared to hypertension, but still exceeded the corresponding value in the control group by 55 % thus resulting in a partial restoration of Na+ affinity of Na,K-ATPase which was depressed as a consequence of NO-dependent hypertension.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Vrbjar N,Javorková V,Pechánová Osubject
Has Abstractpub_date
2002-01-01 00:00:00pages
475-81issue
5eissn
0862-8408issn
1802-9973journal_volume
51pub_type
杂志文章abstract：:Primary aldosteronism (PA) is the most common cause of endocrine hypertension with a high frequency of cardiovascular complications. The unfavorable cardiometabolic profile may be due to aldosterone-mediated activation of inflammatory cells, circulatory cytokines and activation of collagen synthesis in the vessel wall...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932994
更新日期：2016-06-20 00:00:00
abstract：:Cough is one of the most important defensive reflexes. However, extensive non- productive cough is a harmful mechanism leading to the damage of human airways. Cough is initiated by activation of vagal afferents in the airways. The site of their convergence is particularly the nucleus of the solitary tract (nTS). The s...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.934400
更新日期：2020-03-27 00:00:00
abstract：:Slow breathing training reduces resting blood pressure, probably by modifying central autonomic control, but evidence for this is lacking. The pressor response to static handgrip exercise is a measure of autonomic control and the aim of this study was to determine whether slow breathing training modulates the pressor ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932950
更新日期：2015-01-01 00:00:00
abstract：:Obstructive sleep apnea (OSA) has been demonstrated to be implicated in disorder of insulin secretion and diabetes mellitus. In this study, we aimed to evaluate the protective role of tempol, a powerful antioxidant, in chronic intermittent hypoxia (IH)-induced pancreatic injury. The rat model of OSA was established by...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934010
更新日期：2019-06-30 00:00:00
abstract：:An important mechanism underlying cochlear hair cell (HC) susceptibility to hypoxia/ischemia is the influx of Ca(2+). Two main ATP-dependent mechanisms contribute to maintaining low Ca(2+) levels: uptake of Ca(2+) into intracellular stores via smooth endoplasmic reticulum calcium ATPase (SERCA) and extrusion of Ca(2+)...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) has an important role in the pathogenesis of retinopathy of prematurity (ROP) and inhibition of VEGF expression in the neovascular phase might prevent destructive neovascularization in ROP. It is suggested that retinoids exert a highly potent antiangiogenic activity by inhibit...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:A new concept of cardioprotection based on the exploitation of endogenous mechanisms is known as ischemic preconditioning (IPC). It has been hypothesized that substances released during brief ischemic stress (e.g. catecholamines) stimulate the receptors and trigger multiple cell signaling cascades. Opening of ATP-sens...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Numerous countermeasures have been proposed to minimize microgravity-induced physical deconditioning, but their benefits are limited. The present study aimed to investigate whether personalized aerobic exercise based on artificial gravity (AG) mitigates multisystem physical deconditioning. Fourteen men were assigned t...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933493
更新日期：2017-09-22 00:00:00
abstract：:Rat skeletal muscle myofibrils were incubated in the presence of D-glucose, D-fructose, D-galactose, D-ribose, D-tagatose, D-arabinose, D-xylose, D-mannose, L-sorbose, L-rhamnose or DL-glyceraldehyde and myofibrillar ATPase activity as well as the extent of glycation was measured. The attachment of sugars to proteins ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The expression of aquaporins (AQPs) in the fetal porcine urinary tract and its relation to gestational age has not been established. Tissue samples from the renal pelvis, ureter, bladder and urethra were obtained from porcine fetuses. Samples were examined by RT-PCR (AQPs 1-11), QPCR (AQPs positive on RT-PCR), and imm...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933545
更新日期：2018-05-04 00:00:00
abstract：:Nephrotoxicity of cisplatin (CP) involves renal oxidative stress and inflammation, and sesamin (a major liganin in many plants) has strong antioxidant and antiinflammatory actions. Therefore, we investigated here the possible mitigative action of sesamin on CP nephrotoxicity in rats. Sesamin was given orally (5 mg/kg/...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934142
更新日期：2020-02-19 00:00:00
abstract：:Anthropogenic environmental pollutants affect many physiological, biochemical, and endocrine actions as reproduction, metabolism, immunity, behavior and as such can interfere with any aspect of hormone action. Microbiota and their genes, microbiome, a large body of microorganisms, first of all bacteria and co-existing...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934513
更新日期：2020-09-30 00:00:00
abstract：:Our previous studies concerning the role of furosemide-resistant cation leaks in genetic hypertension demonstrated that blood pressure of recombinant inbred strains (derived from F2 hybrids of spontaneously hypertensive and normotensive Brown Norway rats) cosegregated with inward Na+ leak (determined in saline medium)...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Hepcidin, a recently discovered antimicrobial peptide synthesized in the liver, was identified to be the key mediator of iron metabolism and distribution. Despite our knowledge of hepcidin increased in recent years, there are only limited data on hepcidin regulation during systemic inflammatory response in human subje...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.931996
更新日期：2011-01-01 00:00:00
abstract：:The effects of inclusion of sesamin / episesamin in Baltic Atlantic salmon (Salmo salar L.) diets based on vegetable oils were studied. The study was designed as a dose response study with two control diets, one diet based on fish oil (FO) and one diet based on a mixture of linseed and sunflower oil (6:4 by vol.) (MO)...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932068
更新日期：2011-01-01 00:00:00
abstract：:Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids is limited, due to high variability. In this study, we aimed to establish reference values for oxidative stress markers in plasma, urine and saliva of adult, ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933866
更新日期：2018-12-18 00:00:00
abstract：:To compare serum adiponectin changes across the menstrual cycle between normal weight and overweight/obese young women and its correlation with serum estradiol. Young women (n=56) with regular menstrual cycle had been grouped according to their BMI into normal weight group (n=26) and overweight /obese group (n=30). Bl...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934197
更新日期：2019-12-30 00:00:00
abstract：:Osteoporotic fractures are the result of low density and especially inferior bone quality (microarchitecture) caused by both internal (genes, hormones) and external (life style) influences. Bone mechanosensors are extremely important for the overall integrity of the skeleton, because in response to mechanical load the...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2008-01-01 00:00:00
abstract：:The aim of this study was to obtain a detailed analysis of the relationship between the finger arterial compliance C [ml/mm Hg] and the arterial transmural pressure P(t) [mm Hg]. We constructed a dynamic plethysmograph enabling us to set up a constant pressure P(css) [mm Hg] and a superimposed fast pressure vibration ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932929
更新日期：2014-01-01 00:00:00
abstract：:Gain-of-function (GOF) mutations in ion channels are rare events, which lead to increased agonist sensitivity or altered gating properties, and may render the channel constitutively active. Uncovering and following characterization of such mutants contribute substantially to the understanding of the molecular basis of...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.932658
更新日期：2014-01-01 00:00:00
abstract：:We measured plasma concentrations, adipose tissue and placental mRNA expression of hepatokines fetuin A, fetuin B and fibroblast growth factor 21 (FGF21) in 12 healthy pregnant women (P group), 12 pregnant women with gestational diabetes (GDM) and 10 healthy non-pregnant women (N group) to explore their potential role...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934017
更新日期：2018-11-28 00:00:00
abstract：:The effects of 8-days treatment with 17alpha-estradiol (33.3 microg/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was ...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932590
更新日期：2014-01-01 00:00:00
abstract：:Insulin is known to increase blood flow in parallel to glucose uptake in skeletal muscle. However, it is not known if an increase in blood flow by itself is associated with an increase in glucose uptake in the absence of hyperinsulinemia. To investigate further this matter, the effect of increased blood flow on forear...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:We investigated how postural responses to galvanic vestibular stimulation were affected by standing on a translating support surface and by somatosensory loss due to diabetic neuropathy. We tested the hypothesis that an unstable surface and somatosensory loss can result in an increase of vestibulospinal sensitivity. B...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:The biochemical model of excitation-contraction coupling in cardiomyocyte is presented and the validity of simulations of both physiological and pathological processes is discussed. The model of regulatory and actomyosin subsystems, even if it is rather simple in its regulatory subunit, gives results well consistent w...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:We used a rat model to assess the role of nephrin, podocin, and desmin in the pathogenesis of IgA nephropathy (IgAN). A rat IgAN model was established by administration of BSA, CCl(4), and lipopolysaccharide (LPS) and compared with healthy control rats. Urinary protein, urine red blood cells, and biochemical parameter...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932380
更新日期：2013-01-01 00:00:00
abstract：:The mouse kidneys are enlarged after the administration of thyroxine and this influence is not mediated through androgens. The administration of thyroxine increased the weight of the adrenals and the level of plasma corticosterone. Besides the direct effect of the thyroid hormones on the kidney, our findings indicate ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Statins are the most commonly used drugs in patients with dyslipidemia. Among the patients, a significant inter-individual variability with supposed strong genetic background in statin treatment efficacy has been observed. Genome wide screenings detected variants within the CELSR2/PSRC1/SORT1, CILP2/PBX4, APOB, APOE/C...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932341
更新日期：2012-01-01 00:00:00
abstract：:We have investigated the effect of a diet containing of 4% oyster fungus (Pleurotus ostreatus) and 0.1% cholesterol on glycaemia and hyperlipoproteinaemia in rats with insulin-dependent diabetes (streptozotocin 45 mg/kg). After two months, the rats with diabetes kept on the oyster fungus diet, had a significantly lowe...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Experiments were carried out to determine whether there are separate drives from the selected neuronal networks of the brainstem affecting the discharge patterns of laryngeal and respiratory pump muscles during cough. Twenty-four non-decerebrate spontaneously breathing cats anesthetized with sodium pentobarbitone were...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
