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Age-related changes in porcine corticosteroid-binding globulin (pCBG) as determined by an enzyme-linked immunosorbent assay.

Abstract：

:The objectives of this study were to develop an assay for the direct measure of porcine corticosteroid-binding globulin (pCBG) and to confirm age-related changes in plasma pCBG concentration. Isolation and purification of pCBG from plasma was performed by affinity chromatography and HPLC-DEAE anion exchange techniques. Analysis by SDS-PAGE revealed two polypeptides (54 and 59 kDa) having similar amino acid homology (>50%) to previously reported sequences of seven mammalian species for the first 33 amino acids. Porcine CBG (20 ng/well) was immobilized to microtiter plates and standards or samples added along with rabbit antiserum developed against the purified pCBG. Goat anti-rabbit IgG-alkaline phosphatase conjugate was added followed by p-NPP substrate. The resultant color development was read at 405 nm. Intra- and interassay coefficients of variation (n=26) of a pooled sample were 10 and 15%, respectively. Age-related changes (P<0.001) in plasma pCBG concentration (n=203) from day 3 through 168 of age confirmed that, in the pig, changes seen in the percent distribution of cortisol among protein bound and free forms around day 28 of age are associated with an increase in CBG concentration.

journal_name

Domest Anim Endocrinol

journal_title

Domestic animal endocrinology

authors

Roberts MP,Kattesh HG,Baumbach GA,Gillespie BE,Godkin JD,Schneider JF,Saxton AM

doi

10.1016/s0739-7240(03)00011-0

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

323-39

issue

4

eissn

0739-7240

issn

1879-0054

pii

S0739724003000110

journal_volume

24

pub_type

杂志文章

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