Determination of insulin-like growth factor 1 (IGF1) and IGF binding protein levels in swine.

Abstract:

:A heterologous radioimmunoassay system was validated for the determination of IGF1 concentrations in swine sera. Parallelism, accuracy and response to physiological stimuli were obtained following the incubation of serum samples with 1M glycine-glycine HC1 buffer at a pH of 3.5 +/- 0.2 for 24 hours at 37C. Following acidification and neutralization, circulating IGF1 concentrations were significantly (P less than .05) reduced in hypophysectomized swine and elevated in swine injected with porcine growth hormone (pGH) when compared to IGF1 levels in control hogs. IGF binding protein levels were also increased following GH administration and reduced by hypophysectomy. In addition, circulating IGF1 concentrations were significantly (P less than .05) correlated with body size in three types of swine which differ in growth rate and mature body weight. These data suggest that IGF1 is involved in the regulation of swine growth in vivo and that its physiologic regulation is similar to that in humans.

journal_name

Domest Anim Endocrinol

authors

Buonomo FC,Lauterio TJ,Baile CA,Campion DR

doi

10.1016/0739-7240(87)90035-x

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

23-31

issue

1

eissn

0739-7240

issn

1879-0054

pii

0739-7240(87)90035-X

journal_volume

4

pub_type

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