Abstract：
:Reduction in fertility is well known to be possibly related to selenium deficiencies, even if target organ for selenium action is, at present, unclear. The present study was aimed to examine whether selenium directly influences granulosa cells. Bovine granulosa cells from different size follicles were used to investigate the effect of selenium (5 ng/ml), with or without bovine follicle-stimulating hormone (bFSH) (100 ng/ml), on proliferation and steroidogenesis. In addition, we sought to determine if selenium modulates the production of nitric oxide, which is known to play an important role in ovarian activity. Our data demonstrate that selenium significantly (P < 0.001) stimulates the proliferation of the cells from small follicles; moreover, it further potentiates the stimulatory effect of the gonadotropin in the same cells. Furthermore, selenium significantly (P < 0.01) augments E2 output by cells from both kinds of follicles. bFSH increases E2 production (P < 0.01) by cells from large follicles, whereas it exerts a stimulatory (P < 0.01) effect only in the presence of selenium in the cells from the small ones. The production of nitric oxide is significantly increased (P < 0.001) by bFSH, but only in cells from small follicles. Selenium inhibits (P < 0.001) nitric oxide production in cells from both kinds of follicles and significantly decreases (P < 0.001) bFSH-induced nitric oxide production in cells from the small ones. We conclude that selenium acts on granulosa cells by modulating their proliferation and E2 synthesis; moreover, its effect could be mediated, at least in part, through an inhibition of nitric oxide.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Basini G,Tamanini Cdoi
10.1016/s0739-7240(99)00059-4subject
Has Abstractpub_date
2000-01-01 00:00:00pages
1-17issue
1eissn
0739-7240issn
1879-0054pii
S0739-7240(99)00059-4journal_volume
18pub_type
杂志文章abstract：:Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0% (w/w). c...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(91)90020-k
更新日期：1991-10-01 00:00:00
abstract：:beta-Adrenergic receptor (beta-AR) agonists increase muscle mass and decrease body fat in rodents and livestock. With oral administration, however, the effects of beta1-AR and beta2-AR can be different, depending on the species tested. We tested the effects of clenbuterol, a beta2-AR agonist, and ractopamine, a beta1/...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2003.08.004
更新日期：2004-01-01 00:00:00
abstract：:A heterologous radioimmunoassay system was validated for the determination of IGF1 concentrations in swine sera. Parallelism, accuracy and response to physiological stimuli were obtained following the incubation of serum samples with 1M glycine-glycine HC1 buffer at a pH of 3.5 +/- 0.2 for 24 hours at 37C. Following a...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(87)90035-x
更新日期：1987-01-01 00:00:00
abstract：:The feline thyroglobulin promoter was identified by a combination of standard polymerase chain reaction (PCR) techniques, using primers designed according to regions of homology in published sequences from other species, then adaptor ligated PCR. A 310 bp fragment of the feline thyroglobulin promoter was generated, in...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(01)00093-5
更新日期：2001-04-01 00:00:00
abstract：:The objectives of this study were to develop an assay for the direct measure of porcine corticosteroid-binding globulin (pCBG) and to confirm age-related changes in plasma pCBG concentration. Isolation and purification of pCBG from plasma was performed by affinity chromatography and HPLC-DEAE anion exchange techniques...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(03)00011-0
更新日期：2003-05-01 00:00:00
abstract：:The objective of transgenic livestock improvement projects is to develop and bring to market superior breeding stock, as well as germplasm for the artificial insemination and embryo transfer industries. Livestock animal biotechnology programs hold the promise of achieving, in a single generation, improvements in comme...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0739-7240(90)90049-6
更新日期：1990-01-01 00:00:00
abstract：:An increasing demand for calcium during pregnancy and lactation can result in both clinical and subclinical hypocalcemia during the early lactation period in several mammalian species, in particular the dairy cow. Serotonin (5-HT) was recently identified as a regulator of lactation and bone turnover. The purpose of th...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.01.005
更新日期：2013-05-01 00:00:00
abstract：:The site within the hypothalamic-pituitary axis at which cortisol acts to inhibit luteinizing hormone (LH) secretion was investigated in female pigs. Six ovariectomized, hypophysial stalk-transected (HST) gilts were given 1 microgram pulses of gonadotropin releasing-hormone (GnRH) iv every 45 min from day 0 to 12. On ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(91)90008-8
更新日期：1991-07-01 00:00:00
abstract：:The effects of increased postruminal supply of casein, corn starch, and soybean oil on plasma concentrations of the gastrointestinal hormones ghrelin and oxyntomodulin (OXM) were investigated. Four mid-lactation Holstein cows were used in a 4 x 4 Latin square. Treatments were continuous abomasal infusions (23 h/d) for...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2009.12.003
更新日期：2010-05-01 00:00:00
abstract：:Assisted reproductive technologies, including in vitro embryo production (IVP), have been successfully used in animal reproduction to optimize breeding strategies for improved production and health in animal husbandry. Despite the progress in IVP techniques over the years, further improvements in in vitro embryo cultu...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2015.08.003
更新日期：2016-01-01 00:00:00
abstract：:Reduced reproductive performance of lactating cows is strongly associated with environmental and pathogenic stressors. This review summarizes the most recent knowledge on the effects of acute or chronic heat stress (HS) and acute or chronic intramammary infection (IMI) on ovarian function. It also offers various appro...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2016.02.013
更新日期：2016-07-01 00:00:00
abstract：:In swine, colostrum production is induced by the drop of progesterone (P4) concentrations which leads to the prepartum peak of prolactin (PRL). PRL regulates mammary cell turnover and stimulates lacteal nutrient synthesis. P4 inhibits PRL secretion and downregulates the PRL receptor in the mammary gland. The aim of th...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2015.04.005
更新日期：2015-10-01 00:00:00
abstract：:Effects of domperidone, a peripheral dopamine receptor antagonist, on secretion of LH and prolactin were studied during the luteal phase and following administration of PGF2 alpha. Since hyperprolactinemia has been reported to inhibit secretion of LH in ewes, effects of thyrotropin-releasing hormone (TRH) also were ex...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(87)90003-8
更新日期：1987-04-01 00:00:00
abstract：:The corpus luteum (CL) is an ovarian structure which is critical for the maintenance of reproductive cyclicity and pregnancy support. Diet and/or diet components may affect some luteal functions. FSH is widely used to induce multiple follicle development and superovulation. We hypothesized that FSH would affect luteal...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.domaniend.2017.08.002
更新日期：2018-01-01 00:00:00
abstract：:Environment enrichment is a rising topic for animal welfare but measures to identify effective enrichment interventions are lacking. In humans and rodent species, environmental enrichment increases brain-derived neurotrophic factor (BDNF), the most abundant neurotrophin in the brain. Higher BDNF concentration is ultim...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2018.05.007
更新日期：2018-10-01 00:00:00
abstract：:Fifteen cows (87 +/- 8 d in lactation; 641 +/- 33 kg BW) were randomly assigned to treatment and then subjected for 182 d to daily sc injection (1000 hr), in the cervical area, of saline (control), thyrotropin-releasing factor (TRF: 1 micrograms/kg BW), growth hormone-releasing factor (1-29)NH2 (GRF; 10 micrograms/kg ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(91)90044-k
更新日期：1991-01-01 00:00:00
abstract：:It has been established that sows up- or down-regulate their milk production as the frequency of nursings is changed. The amount of udder massage by piglets might also influence milk production. To investigate whether these effects are associated with changes in prolactin or insulin levels, we enforced five sows each ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(99)00025-9
更新日期：1999-07-01 00:00:00
abstract：:The influence of genetic selection for improved feed conversion (FC) and fast growth (GL) in broiler chickens, on the hepatic growth hormone receptor was investigated as a function of age. Selection for improved feed conversion resulted in lean chickens, whereas selection for fast growth resulted in fat chickens. Grow...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(93)90024-6
更新日期：1993-07-01 00:00:00
abstract：:Twelve pregnant Brahman cows were randomly assigned to one of two treatment groups: 1) transported in a stock trailer for 24.2 km, unloaded at a second farm and penned for 1 hr, and then returned to the original farm (TRANS, n = 6); or 2) walked through the handling facilities (SHAM, n = 6). Treatments were repeated a...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(96)00115-4
更新日期：1997-03-01 00:00:00
abstract：:Crossbred ovariectomized ewes were treated with steroid therapies determined previously to be adequate (progesterone-primed) or inadequate (unprimed) for embryonic development in order to determine actual serum concentrations of replaced steroid hormones achieved by such treatments and to identify secreted endometrial...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(93)90017-6
更新日期：1993-04-01 00:00:00
abstract：:The objective of this experiment was to develop a procedure for immunizing ewes against melatonin that would alter the effects of changing photoperiod on seasonal reproduction and prolactin secretion. Ewes were immunized against human serum albumin (HSA) as controls (n = 9) or a melatonin-human serum albumin conjugate...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(99)00017-x
更新日期：1999-05-01 00:00:00
abstract：:In ruminants, both milking frequency and exogenous GH treatment affect milk production. In a previous report, we showed that the modulation of milk yield due to variations in milking frequency and GH treatment was associated with variations in mammary cell numbers. The aim of this study was to clarify the different me...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2004.04.002
更新日期：2004-11-01 00:00:00
abstract：:Nesfatin-1 is a naturally occurring 82-amino acid protein encoded in the precursor nucleobindin-2 (NUCB2) and has been implicated in multiple physiological functions, including food intake and blood glucose regulation. This study aimed to characterize nesfatin-1 in domestic species, especially cats (Felis catus), dogs...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2018.04.006
更新日期：2018-10-01 00:00:00
abstract：:Corpus luteum (CL), a transient endocrine gland critical for reproductive cyclicity and pregnancy maintenance, is controlled by numerous regulatory factors. Although LH is widely recognized as the major regulator, other factors may also affect luteal functions. It has been demonstrated that FSH receptors (FSHR) are ex...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.106391
更新日期：2020-04-01 00:00:00
abstract：:In vitro release of progesterone (P4) and estrone (E1) by the porcine placenta was characterized at 12 days of gestation between d 20 and 110. Placental P4 rose linearly from d 25 to 40, plateaued between d 40 and 50, continued to increase to a peak concentration at d 100, then decreased sharply to d 110. Placental E1...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(90)90007-m
更新日期：1990-10-01 00:00:00
abstract：:Relatively little is known about the physiological roles of microRNAs (miRNAs) during follicular development. Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian folli...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.06.006
更新日期：2013-10-01 00:00:00
abstract：:Leptin, a 16kDa product of the adipose obese (ob) gene, has been shown to contribute to the regulation of energy metabolism, feeding behavior, and reproduction in several monogastric species, including humans. Recent reports have provided evidence that the leptin gene is functionally relevant in cattle and sheep, and ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0739-7240(02)00169-8
更新日期：2002-07-01 00:00:00
abstract：:The objective of the present studies was to determine the effect of cytokines on FSH-induced estrogen production by granulosa cells from small (1-5 mm) and large (> or = 8 mm) bovine follicles. FSH-induced estradiol production by granulosa cells from large follicles (expressed as pg estradiol/10(5) cells/24 hr) was no...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(94)90034-5
更新日期：1994-01-01 00:00:00
abstract：:Glucocorticoids (GCs) are important environmental and maturational signals during intrauterine development. Toward term, the maturational rise in fetal glucocorticoid receptor concentrations decreases fetal growth and induces differentiation of key tissues essential for neonatal survival. When cortisol levels rise ear...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2016.02.014
更新日期：2016-07-01 00:00:00
abstract：:The present study was conducted to evaluate changes in follicular fluid (FF) insulin-like growth factor binding protein (IGFBP) proteolytic activity and levels of steroids and IGFBP during follicular development in cattle. Estrous cycles of cows were synchronized with two injections of prostaglandin F2alpha (PGF) 11 d...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(01)00103-5
更新日期：2001-07-01 00:00:00
