Comparison of six methods for the assessment of ischemia-reperfusion injury in skeletal muscle following composite tissue allotransplantation.

Abstract:

:Ischemia/reperfusion (I/R) injury is one of the factors determining tissue survival in replantation and transplantation surgery. However, more than 20 methods have been used to evaluate I/R injury in muscle. The aim of this study was to examine I/R injury in muscle tissue in a model of composite tissue allotransplantation (rat hindlimb transplantation), based on the analyses of six parameters: nitroblue tetrazolium staining (NBT); histology of the anterior tibial and extensor digitorum muscle; wet-to-dry weight ratio; serum potassium; and serum creatine kinase (CK)), in order to identify the most practicable and reliable outcome parameter. Results demonstrated that NBT staining and the wet/dry weight ratio are reliable tools for outcome measurement. The wet/dry weight ratio is the easiest to perform and the authors consider it to be useful for screening purposes. Histologic assessment shows areas of necrosis, but is not a reliable method for semi-quantitative evaluation. Serum potassium and CK levels were higher following transplantation, but they cannot be recommended for assessment purposes, as no significant correlation with other parameters was seen. These findings help further researchers in their selection of reliable outcome parameters to measure I/R injury in skeletal muscle.

journal_name

J Reconstr Microsurg

authors

Baumeister SP,Ofer N,Kleist C,Rebel M,Dohler B,Terness P,Gebhard MM,Germann G

doi

10.1055/s-2004-823113

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

253-9

issue

3

eissn

0743-684X

issn

1098-8947

journal_volume

20

pub_type

杂志文章
  • Extra and Intramuscular Distribution of the Thoracodorsal Nerve with Regard to Nerve Reconstruction Surgeries.

    abstract::Background The lateral branch of the thoracodorsal nerve (LBTN) is used for nerve transfer in facial, musculocutaneous, axillary nerve injuries and for irreparable C5, C6 spinal nerve lesions and accessory nerve defects. For a successful surgical outcome, the nerve to be used in nerve transfer should be of adequate le...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0036-1579541

    authors: Malalasekera A,Beneragama T,Kanesu S,Sahathevan V,Jayasekara R

    更新日期:2016-06-01 00:00:00

  • Autologous denatured muscle as a nerve graft.

    abstract::This study examined the use of freeze-thawed muscle as a nerve graft material in Sprague-Dawley rats. In Group 1 (n = 4), the sciatic nerve was isolated and the incision immediately closed. In Group 2 (n = 4), a 5-mm segment of the nerve was removed and immediately replaced. In Group 3 (n = 50), a 5-mm segment of nerv...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006580

    authors: DeFranzo AJ,Morykwas MJ,LaRosse JR,Jennings DA,Challa V,Argenta LC

    更新日期:1994-05-01 00:00:00

  • Donor-site morbidity of the sensate extended lateral arm flap.

    abstract::The free extended lateral arm flap (ELAF) has gained increasing popularity thank to its slimness and versatility, longer neurovascular pedicle, and greater flap size when compared with the original flap design. The aim of this study was to assess the donor-site morbidity associated with this extended procedure. A retr...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0031-1289165

    authors: Depner C,Erba P,Rieger UM,Iten F,Schaefer DJ,Haug M

    更新日期:2012-02-01 00:00:00

  • Microarterial anastomosis with a distal tapering technique.

    abstract::In microsurgical arterial anastomoses, there are some occasions in which the diameter of the distal artery is much larger than that of the proximal one. A fish-mouth incision technique is useful when there is considerable size discrepancy. However, this method cannot be used when the distal artery is much larger than ...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006576

    authors: Ueda K,Harashina T,Inoue T,Kurihara T,Harada T,Oba S

    更新日期:1994-03-01 00:00:00

  • Cortical brain mapping of peripheral nerves using functional magnetic resonance imaging in a rodent model.

    abstract::The regions of the body have cortical and subcortical representation in proportion to their degree of innervation. The rat forepaw has been studied extensively in recent years using functional magnetic resonance imaging (fMRI), typically by stimulation using electrodes directly inserted into the skin of the forepaw. H...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0028-1088231

    authors: Cho YR,Jones SR,Pawela CP,Li R,Kao DS,Schulte ML,Runquist ML,Yan JG,Hudetz AG,Jaradeh SS,Hyde JS,Matloub HS

    更新日期:2008-11-01 00:00:00

  • Reliability of Various Predictors for Preoperative Diagnosis of Infraclavicular Brachial Plexus Lesions with Shoulder and/or Elbow Paresis.

    abstract:BACKGROUND: The distinction between supraclavicular and infraclavicular acute brachial plexus injuries (BPIs) could be challenging in cases of combined shoulder and elbow paresis. The reliability of several preoperative predictors was investigated to avoid unnecessary dissection, prolonged operation time, increased pos...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0040-1702177

    authors: Qiu SS,Chang TN,Lu JC,Chuang DC

    更新日期:2020-07-01 00:00:00

  • Outcomes of Head and Neck Microvascular Reconstruction in Hypercoagulable Patients.

    abstract:BACKGROUND: Inherited coagulopathies and previous thrombotic events are often considered relative contraindications to microvascular reconstruction. We hypothesize that with planning, head and neck microvascular reconstruction can be successfully performed in hypercoagulable individuals. METHODS: A retrospective revie...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0039-3401846

    authors: Nguyen TT,Egan KG,Crowe DL,Nazir N,Przylecki WH,Andrews BT

    更新日期:2020-05-01 00:00:00

  • A Novel Method of Neo-osseous Flap Prefabrication: Induction of Free Calvarial Periosteum with Bioactive Glass.

    abstract:BACKGROUND: Reconstruction of craniofacial bone defects is a primary focus of craniofacial surgery. Although autogenous bone grafts remain as the gold standard, alloplastic materials have also gained widespread popularity due to their off-the-shelf availability, ease of use, and durability. In addition to replacing the...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0037-1598254

    authors: Aliyev A,Ekin Ö,Bitik O,Korkusuz P,Yersal NC,Çelik HH,Tunçbilek G

    更新日期:2018-06-01 00:00:00

  • Anatomic variations in branching patterns of the axillary artery: a multidetector-row computed tomography angiography study.

    abstract::Anatomic variations in branching pattern of axillary artery (AxA) are common and typically involve subscapular artery (SsA) and posterior circumflex humeral artery (PCHA). Several skin and muscle flaps are based on the branches of AxA. Furthermore, these branches are frequently used as recipient vessels in functioning...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0033-1351354

    authors: Hattori Y,Doi K,Sakamoto S,Satbhai N

    更新日期:2013-10-01 00:00:00

  • The influence of magnitude and duration of crush load on functional recovery of the peripheral nerve.

    abstract::This study investigated the effect of crush duration at different loads on motor functional recovery. One hundred and thirty-eight rats were divided into five groups: sham operation, resected sciatic nerve, and 100 g, 500 g, and 15,000 g of sciatic crush load (Groups 1 to 5). According to crush duration, Groups 3 and ...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006671

    authors: Chen LE,Seaber AV,Urbaniak JR

    更新日期:1993-07-01 00:00:00

  • Measuring the pressure in the superficial inferior epigastric vein to monitor for venous congestion in deep inferior epigastric artery perforator breast reconstructions: a pilot study.

    abstract::During deep inferior epigastric artery perforator (DIEP) flap dissection, we noted that in many cases the superficial vein on the ipsilateral side of the flap was engorged and tense, and in others, it was empty. This led us to believe that the pressure is increased as the result of preferential outflow through the sup...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0029-1243294

    authors: Smit JM,Audolfsson T,Whitaker IS,Werker PM,Acosta R,Liss AG

    更新日期:2010-02-01 00:00:00

  • Prolonging survival in vascularized bone allograft transplantation: developing specific immune unresponsiveness.

    abstract::Vascularized bone allografts (VBAs) could be useful adjuncts to the clinical reconstructive surgeon's arsenal. These grafts are known experimentally to be subject to host rejection. One way to control the rejection problem would be to develop specific immune unresponsiveness via host conditioning. Using a proven relia...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006992

    authors: Paskert JP,Yaremchuk MJ,Randolph MA,Weiland AJ

    更新日期:1987-04-01 00:00:00

  • Carpal tunnel syndrome in long-term hemodialyzed patients.

    abstract::The etiopathology, clinical features, and treatment of carpal tunnel syndrome (CTS) in long-term hemodialyzed patients, are discussed in the light of new clinical findings. Conclusions summarizing the specific characteristics of CTS in hemodialyzed patients include the following. 1) There is a relatively equal sex rat...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006856

    authors: Corradi M,Paganelli E,Pavesi G

    更新日期:1989-04-01 00:00:00

  • Successful replantation of the lower leg after 42-hour ischemia: case report.

    abstract::A case of right lower-leg replantation after 42-hr ischemia is presented. Revascularization of the other foot with circulatory decompensation after 36-hr ischemia was carried out simultaneously. The replanted lower leg survived. Following its shortening in replantation by 12 cm, right lower-leg lengthening by 8 cm was...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006729

    authors: Datiashvili RO,Chichkin VG

    更新日期:1992-11-01 00:00:00

  • Free vascularized fibula grafts in surgery of the upper limb.

    abstract::Twenty patients with intractable diseases in the upper extremity were treated using free vascularized fibula grafts. There were 13 men and seven women. Three patients had traumatic bone defects, five had post-traumatic nonunions, two had congenital pseudoarthroses, seven had defects after tumor resection, and three ha...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1000131

    authors: Yajima H,Tamai S,Ono H,Kizaki K,Yamauchi T

    更新日期:1999-10-01 00:00:00

  • Blood-flow contributions by the superior and inferior epigastric arterial systems in TRAM flaps, based on laser Doppler flowmetry.

    abstract::This study was designed to quantify the relative contribution of the inferior and superior epigastric systems to infraumbilical TRAM flap skin blood flow, using laser Doppler flowmetry. In twelve flaps (11 patients), blood-flow measurements were obtained during routine elevation of the TRAM flap for breast reconstruct...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006593

    authors: Hendricks DL,Wilkens TH,Witt PD

    更新日期:1994-07-01 00:00:00

  • Preclinical efficacy of slow-release bFGF in ischemia-reperfusion injury in a Dorsal Island skin flap model.

    abstract::The effect of slow-release basic fibroblast growth factor (bFGF) on ischemia-reperfusion injury was examined using an island skin flap model in rats. Paired rectangular island skin flaps were elevated on the dorsum of 30 Fischer rats. The flaps were subjected to 6 hours of ischemia. Before reperfusion the flaps were i...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0033-1343830

    authors: Wang Y,Orbay H,Huang C,Tobita M,Hyakusoku H,Myamoto M,Tabata Y,Mizuno H

    更新日期:2013-06-01 00:00:00

  • Reporting Practices on Immunosuppression and Rejection Management in Face Transplantation: A Systematic Review.

    abstract:BACKGROUND: Face transplantation is a demanding operation requiring complex planning and expert technical performance. While the documentation of successes of the first transplants is impressive, there are lacunae in reporting of institutional protocols for other critical operation components, namely, immunosuppression...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0039-1691787

    authors: Daneshgaran G,Stern CS,Garfein ES

    更新日期:2019-11-01 00:00:00

  • Patellar tendon reconstruction using a free latissimus dorsi flap following resection of a prepatellar myxofibrosarcoma: case report.

    abstract::The authors' experience indicates that good functional results are achievable in cases with wide resection of the knee extensor mechanism. They report a low-grade myxoid fibrosarcoma patient in whom a one-stage functional reconstruction was carried out by transfer of a free latissimus dorsi flap without secondary surg...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2005-871749

    authors: Machens HG,Siemers F,Kaun M,Krapohl B,Reichert B,Russlies M,Krüger S,Stöckelhuber B,Mailänder P

    更新日期:2005-05-01 00:00:00

  • Double level amputation: should it be replanted?

    abstract::Multiple level amputation has been described as a contraindication to replantation. This is a case report of a patient with a double level amputation through the palm and forearm that was successfully replanted. Because of the need for multiple stages of reconstructive surgery, it was not clear until the end result th...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1007016

    authors: Belsky MR,Ruby LK

    更新日期:1986-04-01 00:00:00

  • Forequarter amputation wound coverage with an ipsilateral, lymphedematous, circumferential forearm fasciocutaneous free flap in patients undergoing palliative shoulder-girdle tumor resection.

    abstract::Closure of massive soft-tissue defects in patients undergoing forequarter amputation for shoulder-girdle tumors may present a daunting challenge. This report describes two patients whose forequarter amputations were closed using ipsilateral, lymphedematous, circumferential forearm fasciocutaneous free flaps. ...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-1006658

    authors: Zachary LS,Gottlieb LJ,Simon M,Ferguson MK,Calkins E

    更新日期:1993-03-01 00:00:00

  • The vastus intermedius periosteal (VIP) flap: a novel flap for osteoinduction.

    abstract::Periosteum's role in fracture healing is widely recognized, and its function in bone tissue engineering shows great potential. Here we introduce a novel periosteal free flap to be used as an abundant source of periosteum in the engineering and repair of bone. The descending branch of the lateral femoral circumflex ves...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0030-1249317

    authors: Maercks RA,Runyan CM,Jones DC,Taylor JA

    更新日期:2010-07-01 00:00:00

  • Effect of systemic piracetam treatment on flap survival and vascular endothelial growth factor expression after ischemia-reperfusion injury.

    abstract::The effects of piracetam on flap survival, ischemia-reperfusion (I/R) injury, and vascular endothelial growth factor (VEGF) expression were evaluated in this study. Unipedicled epigastric flap model was used in 36 rats and was evaluated within 4 groups. The flap was elevated and untreated in Group 1. Postoperative pir...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0031-1281523

    authors: Tuncer S,Ayhan S,Findikcioglu K,Ergun H,Tuncer I

    更新日期:2011-09-01 00:00:00

  • L-arginine reduces neointimal hyperplasia in cold-stored arterial allografts in a rabbit low-flow-through model.

    abstract::An "off the shelf" vascular conduit for free flap surgery that remained patent until the flap is vascularized from the recipient bed would be useful to avoid autograft donor-site morbidity and reduce operation time. To date, no successful studies of microvascular prostheses in low-flow states exist. This study investi...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-985210

    authors: Dowd MB,Hemmrich K,Morrison WA

    更新日期:2007-08-01 00:00:00

  • Long-term results of lateral calcaneal artery flap for hindfoot reconstruction.

    abstract::The purpose of the present study was to analyze our long-term results of lateral calcaneal artery flap transfer for hindfoot reconstruction. A total of four patients (average age, 48 years) underwent lateral calcaneal artery flap transfer. The etiologies were heat injury of the lateral malleolus in one patient and ski...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2008-1078690

    authors: Omokawa S,Yajima H,Tanaka Y,Ryu J

    更新日期:2008-05-01 00:00:00

  • Facial nerve reconstruction using a vascularized lateral femoral cutaneous nerve graft based on the superficial circumflex iliac artery system: an application of the inferolateral extension of the groin flap.

    abstract::The use of an inferolateral extension technique of a groin flap has previously been reported. This technique involves harvesting an extended portion from the anterolateral thigh, including the lateral femoral cutaneous nerve (LFCN) and its accompanying vessels, attached to a groin flap via communications between the L...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0030-1263288

    authors: Kashiwa K,Kobayashi S,Nasu W,Kuroda T,Higuchi H

    更新日期:2010-11-01 00:00:00

  • FK506 and nerve regeneration: past, present, and future.

    abstract::Promoting neurological recovery requires strategies that simultaneously provide protection to injured neurons and increase the numbers of neurons that extend axons while inducing more rapid and extensive axon regeneration across long nerve gaps. An agent that facilitates the speed and success of reinnervation will hav...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章,评审

    doi:10.1055/s-0032-1333314

    authors: Konofaos P,Terzis JK

    更新日期:2013-03-01 00:00:00

  • Reconstruction of large traumatic segmental defects of the femur using segmental allograft with vascularized fibula inlay.

    abstract::Segmental defects of the distal femur following trauma pose a reconstructive challenge. A stable reconstruction capable of withstanding high forces while allowing early mobility is paramount. The Capanna technique of reconstruction combining allograft with vascularized bone graft provides such a construct and has been...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0031-1281520

    authors: Ridha H,Bernard J,Gateley D,Vesely MJ

    更新日期:2011-07-01 00:00:00

  • Indocyanine Green Lymphography, Lymphoscintigraphy, and Genetic Analysis in Nonsyndromic Primary Lymphedema: The Distal Dermal Backflow Grading System and the Print Sign.

    abstract:BACKGROUND: Investigating correlations between clinical, instrumental, and genetic features of primary lymphedema (PL) with the aim to facilitate the diagnosis, the staging, and the management of this subgroup of patients. METHODS: A prospective observational study was conducted from September 2016 to May 2018, includ...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-0039-1698748

    authors: Mangialardi ML,Lorenzano V,Pagliara D,Visconti G,Gentileschi S,Salgarello M

    更新日期:2020-03-01 00:00:00

  • Single-stage maxillary and nasal floor reconstruction with the double-paddle rectus abdominis musculocutaneous free flap.

    abstract::Palatal integrity is essential for useful speech, deglutition, good oral hygiene, and prevention of nasal regurgitation. Maxillary defects after tumor extirpation, therefore, can have serious functional and cosmetic implications. Given the often disappointing results obtained with local and regional pedicled flaps for...

    journal_title:Journal of reconstructive microsurgery

    pub_type: 杂志文章

    doi:10.1055/s-2007-974647

    authors: Herman CK,Benacquista T,Brindzei N,Berdichevsky M,Baum T,Strauch B

    更新日期:2007-04-01 00:00:00