Autologous denatured muscle as a nerve graft.

Abstract:

:This study examined the use of freeze-thawed muscle as a nerve graft material in Sprague-Dawley rats. In Group 1 (n = 4), the sciatic nerve was isolated and the incision immediately closed. In Group 2 (n = 4), a 5-mm segment of the nerve was removed and immediately replaced. In Group 3 (n = 50), a 5-mm segment of nerve was removed and muscle (2 x 2 x 5 mm, harvested from the gluteus, frozen in liquid nitrogen, and thawed in sterile water) was sutured in place. The short 5-mm segment of muscle provided an ideal situation for regeneration of myelinated nerve. Sciatic functional indices were comparable in all groups after 8 weeks and at 1 year. Histologic analysis showed comparable nerve regeneration in Groups 2 and 3 at 4 months. Group 3 grafts exhibited new axonal growth distal to the repaired gap at 4 weeks; myelinated fibers were present at 10 weeks. At 1 year, Group 3 nerves had almost the same axon count as contralateral nerves. However, myelin-sheath regeneration in the 5-mm muscle graft was incomplete, at 81 percent of normal, at 1 year.

journal_name

J Reconstr Microsurg

authors

DeFranzo AJ,Morykwas MJ,LaRosse JR,Jennings DA,Challa V,Argenta LC

doi

10.1055/s-2007-1006580

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

145-9; discussion 149-51

issue

3

eissn

0743-684X

issn

1098-8947

journal_volume

10

pub_type

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