Abstract:
:A specific, and automated method was developed to quantitate neomycin in human serum. Samples were prepared with an automated solid phase extraction (SPE). The hydrophilic interaction chromatography (HILIC) was used for additional sample cleanup and baseline separation. The analyte neomycin was detected with electrospray ionisation tandem mass spectrometry (ESI-MS-MS). Using a volume of 500 microl biological sample the lower limit of quantification was 100 ng/ml. The described HILIC-MS-MS method is suitable for clinical and pharmcokinetical investigations of neomycin.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Oertel R,Renner U,Kirch Wdoi
10.1016/j.jpba.2004.01.018subject
Has Abstractpub_date
2004-05-28 00:00:00pages
633-8issue
3eissn
0731-7085issn
1873-264Xpii
S0731708504000408journal_volume
35pub_type
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