Vascular cell adhesion molecule-1-targeted detection of endothelial activation in human microvasculature.


:The hallmark of endothelial activation, an early and critical step in many alloimmune and inflammatory responses, is the transcriptional induction and expression of endothelial adhesion molecules (eg, vascular cell adhesion molecule-1 [VCAM-1]). We assessed the feasibility of VCAM-1-targeted in vivo detection of endothelial activation using I-125-labeled-F(ab')2 fragments of E1/6, a monoclonal antibody against human but not murine VCAM-1. The Kd and Bmax, determined by saturation binding in tumor necrosis factor (TNF)-activated human endothelial cells (ECs), were 3.2 +/- 0.6 nmol/L and 5600 +/- 300 binding sites per EC, respectively. Biodistribution and in vivo binding characteristics of I-125-E1/6 F(ab')2 were assessed in a novel chimeric human/mouse model, in which human skin (as a source of human microvasculature) is grafted onto SCID/beige mice. I-125-E1/6 F(ab')2 localized to TNF-activated human skin grafts as detected by autoradiography and gamma well-counting. Relative uptakes (uptake in human skin graft/uptake in the surrounding mouse skin) were, respectively, 2.6 +/- 0.8 (n = 14) and 1.6 +/- 0.3 (n = 12) for E1/6 and MOPC-21, an isotype-matched control antibody (P < .01). The preferential uptake in human skin graft was not due to differences in tissue vascularity assessed by Tc-99m-labeled murine red blood cells. In conclusion, the chimeric human/mouse model is a novel experimental tool for in vivo evaluation of human endothelial cell-specific radiopharmaceuticals. Although I-125-E1/6 F(ab')2 localized to human skin grafts, the limited number of VCAM-1 molecules/endothelial cell adversely affects its suitability as a target for in vivo imaging of endothelial activation.


Transplant Proc


Sadeghi MM,Schechner JS,Krassilnikova S,Gharaei AA,Zhang J,Kirkiles-Smith N,Sinusas AJ,Zaret BL,Bender JR




Has Abstract


2004-06-01 00:00:00














  • Chronic intestinal pseudo-obstruction related to viral infections.

    abstract::Chronic intestinal pseudo-obstruction (CIPO), one of the most severe gastrointestinal motility disorders, is a condition characterized by a clinical picture mimicking small bowel occlusion with related symptoms and signs in the absence of demonstrable mechanical obstruction. Analysis of full-thickness biopsy samples m...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: De Giorgio R,Ricciardiello L,Naponelli V,Selgrad M,Piazzi G,Felicani C,Serra M,Fronzoni L,Antonucci A,Cogliandro RF,Barbara G,Corinaldesi R,Tonini M,Knowles CH,Stanghellini V

    更新日期:2010-01-01 00:00:00

  • Toxoplasmic Encephalitis Followed by Primary EBV-Associated Post-Transplant Lymphoproliferative Disorder of the Central Nervous System in a Patient Undergoing Allogeneic Hematopoietic Stem Cell Transplant: A Case Report.

    abstract::Toxoplasmic encephalitis (TE) and post-transplant lymphoproliferative disorder of the central nervous system (CNS-PTLD) are major complications after allogeneic hematopoietic stem cell transplant (allo-SCT); both are fatal without timely diagnosis and disease-specific treatment. Differential diagnosis of TE and CNS-PT...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Mayumi A,Yamashita T,Matsuda I,Hikosaka K,Fujino S,Norose K,Kato Y,Hirota S,Nakajima T,Ogawa H,Ikegame K

    更新日期:2020-11-01 00:00:00

  • Isolated Pulmonary Cryptococcosis Confused with Lung Tumor 5 Years After Kidney Transplantation: A Case Report.

    abstract:BACKGROUND:In transplant recipients, due to the use of immunosuppressive therapy, it is occasionally difficult to distinguish between an infection and malignancy, especially in the case of a lung lesion. Here, we report a case of isolated pulmonary cryptococcosis after kidney transplantation that was difficult to disti...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Banshodani M,Marubayashi S,Shintaku S,Moriishi M,Tsuchiya S,Ohdan H,Kawanishi H

    更新日期:2019-03-01 00:00:00

  • N-Acetylcysteine Use in Hepatic Ischemia/Reperfusion in Rats Minimizing Bowel Injury.

    abstract:BACKGROUND:The ischemia/reperfusion (I/R) phenomenon can cause the dysfunction of some transplanted organs and other distant organs. Liver surgery success, including transplantations, may depend on the adverse effects of intestinal mucosa injury arising from temporary porta triad occlusion. The study objective was to e...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Lauz Medeiros SH,de Oliveira Menezes A,Zogbi L,Frasson de Souza Montero E

    更新日期:2016-09-01 00:00:00

  • Deceased Donor Liver Transplantation in India in the COVID-19 Era: Current Scenario and Future Perspectives.

    abstract::Coronavirus disease 2019 (COVID-19) has been recently declared a global pandemic. As of June 5, 2020, over 75,000 cases have been reported with nearly 2500 deaths in India alone. COVID-19 has severely impacted deceased donor liver transplant (DDLT) programs throughout the world. Acceptance of deceased liver donors has...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Pahari H,Shellagi N,Nath B

    更新日期:2020-11-01 00:00:00

  • Long-term results of monoclonal anti-Il2-receptor antibody versus polyclonal antilymphocyte antibodies as induction therapy in renal transplantation.

    abstract::We compared the influence of induction therapy on 5-year patient and graft survival as well as on renal function in 100 kidney graft recipients at low immunological risk treated with antilymphocyte globulin (n = 50) versus anti-IL-2R monoclonal antibody (n = 50) in a prospective multicenter study. Long-term immunosupp...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Al Najjar A,Etienne I,Le Pogamp P,Bridoux F,Le Meur Y,Toupance O,Mousson C,Caillard S,Hurault de Ligny B,Marlière JF,Lebranchu Y

    更新日期:2006-09-01 00:00:00

  • Simultaneous pancreas-kidney transplantation: infectious complications and microbiological aspects.

    abstract:OBJECTIVE:The purpose of this study was to describe the clinical and microbiological characteristics of the infectious complications among simultaneous pancreas-kidney transplantations (SPKT). MATERIALS AND METHODS:Among the first 45 SPKT the mean age was 34 years (range, 21 to 49) and the mean duration of follow-up 1...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Linhares MM,Gonzalez AM,Triviño T,Barbosa MM,Schraibman V,Melaragno C,Moura RM,Silva MH,Sá JR,Aguiar WF,Rangel EB,Serra CB,Succi T,Pestana JO

    更新日期:2004-05-01 00:00:00

  • Nitric oxide and thromboxane A2 modulate pulmonary pressure after ischemia and intestinal reperfusion.

    abstract:INTRODUCTION:Nitric oxide (NO) and thromboxane A(2) (TxA(2)) are paracrine substances that likely contribute to IR-induced lung injury. This study examined the hypothesis that pulmonary vasoconstriction during ischemia is induced by NO synthesis and ischemia/reperfusion (IR)-induced TxA(2). METHODS:Wistar rats underwe...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Słupski M,Szadujkis-Szadurska K,Szadujkis-Szadurski R,Szadujkis-Szadurski L,Włodarczyk Z,Andruszkiewicz J,Sinjab AT

    更新日期:2006-01-01 00:00:00

  • European homograft bank: twenty years of cardiovascular tissue banking and collaboration with transplant coordination in Europe.

    abstract::Established in 1989 in Brussels as an international nonprofit association, the European Homograft Bank (EHB) has been collaborating closely with the transplant coordination of the different centers in Belgium and other European countries. Donor selection is made after discussion of exclusion criteria with the transpla...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Jashari R,Goffin Y,Vanderkelen A,Van Hoeck B,du Verger A,Fan Y,Holovska V,Brahy O

    更新日期:2010-01-01 00:00:00

  • Successful kidney transplantation in a patient with multiple endocrine neoplasia type 2A syndrome: case study.

    abstract::We present the case of a 37-year-old patient diagnosed with multiple endocrine neoplasia type 2A (MEN 2A) syndrome, as confirmed by genetic tests, who underwent the transplantation of a kidney from a cadaveric donor. MEN 2A, a hereditary autosomal dominant syndrome, is caused by the mutation of the RET proto-oncogene....

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Rudzki S,Matuszek M,Bicki J,Furmaga J,Pilat J,Gieryng J

    更新日期:2011-10-01 00:00:00

  • Use of Prograf (FK 506) as rescue therapy for refractory rejection after liver transplantation. US Multicenter FK 506 Liver Study Group.

    abstract::This report describes the clinical characteristics and demographics of patients enrolled into this rescue trial for patients experiencing refractory rejection after liver transplantation. Actuarial graft and patient survival at 12 months postconversion was 50% and 72%, respectively. Actual treatment success at 3 month...

    journal_title:Transplantation proceedings

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Klintmalm GB,Goldstein R,Gonwa T,Wiesner RH,Krom RA,Shaw BW Jr,Stratta R,Ascher NL,Roberts JW,Lake J

    更新日期:1993-02-01 00:00:00

  • Renal event outcomes in intestinal transplantation: results from a single-center experience.

    abstract:BACKGROUND:Poor patient outcomes have been closely linked with perioperative renal function after most solid organ transplants, except intestinal transplantation (ITx). This study examined the effect of peri-ITx renal function on outcome. PATIENTS AND METHODS:A retrospective review of all patients undergoing ITx since...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Kaldas F,Farmer D,Gordon SA,McDiarmid SV,Venick R,Colangelo J,Busuttil RW

    更新日期:2007-12-01 00:00:00

  • The family interview in the process of donating organs and tissues for transplantation: perceptions of potential donors' relatives.

    abstract:BACKGROUND:The family interview is a complex phase of the organ donation process because it involves aspects of the interviewer, the interviewee, the interview location, and ethical and legal issues. However, there are few publications on this phase of the donation process. This study aimed to reveal the meaning assign...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: dos Santos MJ,Massarollo MC,de Moraes EL

    更新日期:2014-07-01 00:00:00

  • Bacteriocins from lactic acid bacteria increases tumor necrosis factor-alpha expression in a rat kidney model of chronic rejection.

    abstract::Chronic allograft dysfunction is the primary cause of graft loss after the first posttransplantation year. Bacteriocins are biologically active proteins exhibiting antimicrobial properties against other bacterial species, which are usually closely related to the producer organism. The objective of our study was to det...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Zhu LH,Li C,Wu JA,Liang JG,Shi YF

    更新日期:2008-12-01 00:00:00

  • Hybrid procedure in living donor liver transplantation.

    abstract:BACKGROUND:We have previously reported a hybrid procedure that uses a combination of laparoscopic mobilization of the liver and subsequent hepatectomy under direct vision in living donor liver transplantation (LDLT). We present the details of this hybrid procedure and the outcomes of the procedure. METHODS:Between Jan...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Soyama A,Takatsuki M,Hidaka M,Adachi T,Kitasato A,Kinoshita A,Natsuda K,Baimakhanov Z,Kuroki T,Eguchi S

    更新日期:2015-04-01 00:00:00

  • Early administration of FTY720 prevents chronic airway as well as vascular destruction in experimental rat lung transplantation.

    abstract:BACKGROUND:Chronic rejection (CR) in terms of bronchiolitis obliterans (BO) and vascular sclerosis (VS) still represents the major obstacle for pulmonary graft survival in the medium and long term course after lung transplantation (LTX). Aside from nonspecific stimuli, early acute rejection (AR) seems to be causative e...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Hirt SW,von Suesskind-Schwendi M,Puehler T,Schmid C,Lehle K

    更新日期:2013-03-01 00:00:00

  • Biliary tract complications after orthotopic liver transplantation: still the "Achilles heel"?

    abstract::Postoperative biliary tract complications after liver transplantation (LT) still lead to early and late morbidity and mortality. Modern interventional endoscopic techniques can replace surgical repair as the first line of treatment. Nevertheless surgical intervention plays an important role in specific situations. We ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Perrakis A,Förtsch T,Schellerer V,Hohenberger W,Müller V

    更新日期:2010-12-01 00:00:00

  • How long should we follow the post-transplantation patient after graft loss? A case report of renal cancer in the grafted kidney that occurred 16 years after graft loss.

    abstract:BACKGROUND:Renal cancers commonly occur in the native kidneys of renal transplant recipients, whereas renal cancer in the grafted kidney has been reported occasionally. Renal cancer in the grafted kidney occurred 16 years after graft loss in this case, which would be a more rare case. CASE REPORT:A 60-year-old man who...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Akioka K,Masuda K,Harada S,Nakao T,Nakamura T,Okugawa K,Nakano K,Osaka Y,Tsuchiya K,Sako H

    更新日期:2014-01-01 00:00:00

  • Static Cold Storage vs Ex Vivo Machine Perfusion: Results From a Comparative Study on Renal Transplant Outcome in a Controlled Donation After Circulatory Death Program.

    abstract:INTRODUCTION:We aimed to evaluate if ex vivo machine perfusion could minimize the negative impact of cold ischemia on those renal grafts obtained from controlled donation after circulatory death (cDCD). MATERIAL AND METHODS:Prospective observational paired study of kidney transplants from cDCD performed in our center....

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Arlaban M,Barreda P,Ballesteros MA,Rodrigo E,Suberviola B,Valero R,Miñambres E,Ruiz-San Millán JC

    更新日期:2019-03-01 00:00:00

  • Role of regulated upon activation normal T-cell expressed and secreted in a model of retransplantation acute rejection mediated by alloreactive memory CD4+ T cells.

    abstract:BACKGROUND:It is unknown what role Regulated Upon Activation Normal T-Cell Exposed and Secreted may play in retransplantation or T-cell memory-transfer models. The experiment observed the influence of the chemokine RANTES in a mouse model of acute cardiac allograft rejection induced by adoptive transfer of alloreactive...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Zhou X,Shan Z,Liang H,Lin Z,Qiu S,Kuang F,Zhuang J,Qi Z

    更新日期:2013-03-01 00:00:00

  • A new kidney allocation policy in Chile: computer-based simulations.

    abstract:INTRODUCTION:Kidney allocation should reach a balance between equity and efficiency. In Chile kidneys are allocated based on ABO matching, first to medical priorities and then according to a point scheme considering human leukocyte antigen (HLA) match (60%), waiting list time (20%), and panel-reactive antibodies (PRA; ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Domínguez J,Harrison R,Atal R,Muñoz F

    更新日期:2013-05-01 00:00:00

  • Pancreatic transdifferentiation in porcine liver following lentiviral delivery of human furin-cleavable insulin.

    abstract::Type I diabetes mellitus (TID) results from the autoimmune destruction of the insulin-producing pancreatic β-cells. Gene therapy is one strategy being actively explored to cure TID by affording non-β-cells the ability to secrete insulin in response to physiologic stimuli. In previous studies, we used a novel surgical ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Gerace D,Ren B,Hawthorne WJ,Byrne MR,Phillips PM,O'Brien BA,Nassif N,Alexander IE,Simpson AM

    更新日期:2013-06-01 00:00:00

  • Heart transplantation during active infective endocarditis: case report and review of the literature.

    abstract::Bacterial infections are a contraindication to organ transplantation, but infective endocarditis may require heart transplantation when otherwise untreatable. We describe a heart transplant patient with cardiomyopathy and ongoing defibrillator endocarditis due to Staphylococcus epidermidis. An initial attempt at percu...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章,评审


    authors: Durante-Mangoni E,Casillo R,Pinto D,Caianiello C,Albisinni R,Caprioli V,Maiello C,Utili R

    更新日期:2011-01-01 00:00:00

  • Cyclosporine monitoring in stable, long-term, pediatric kidney transplant recipients: the value of C2 determination.

    abstract::Although a generalized consensus has been reached for therapeutic drug monitoring of cyclosporine microemulsion in adult transplant patients, clear guidelines are recently not available for the pediatric population. In this retrospective analysis of pharmacokinetic data obtained from stable, long-term, pediatric kidne...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Ferraresso M,Ghio L,Tirelli S,Pedotti P,Taioli E,Edefonti A,Berardinelli L

    更新日期:2004-04-01 00:00:00

  • Results of Haberal's Corner-Saving Ureteral Anastomosis Technique for Renal Transplantation: A Retrospective Study.

    abstract:BACKGROUND:Urological complications after renal transplantation (RT), including urine leaks, remain the most common type of surgical complications in the early post-transplant period. In this study we evaluated 324 consecutive RTs recipients in whom Haberal's corner-saving anastomosis technique was used for ureteroneoc...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sevmis M,Kilercik H,Aktas S,Alkara U,Demir ME,Sevmis S

    更新日期:2020-11-28 00:00:00

  • Renal transplantation in human immunodeficiency virus-positive patients: a report of four cases.

    abstract:INTRODUCTION:Renal transplantation (RT) in patients infected with human immunodeficiency virus (HIV) has significantly improved under the advent of combined antiretroviral therapy (cART). The authors describe their experience in RT in patients with HIV from September 2010 to June 2013. CASES REPORT:Four patients under...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Nunes AT,Pereira L,Cerqueira A,Bustorff M,Sampaio S,Ferreira I,Tavares I,Santos J,Pestana M

    更新日期:2014-07-01 00:00:00

  • Cholangiocarcinoma, 10-Year Survival After Liver Transplantation: A Case Report.

    abstract::Cholangiocarcinoma is the second most common neoplasm in the liver, with a very poor, short-term prognosis. Today, surgery associated with or without an adjuvant is the only curative treatment. Liver transplantation (LT) is the best treatment for hepatocellular carcinoma tumor. In recent years, treatment of hilar chol...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Herrero Torres MA,Dominguez Bastante M,Molina Raya A,Palomo Lopez I,Santoyo Villalba J,Villegas Herrera MT,Becerra Massare A,Brea Gomez E,Zambudio Carroll N,Villar Del Moral JM

    更新日期:2020-03-01 00:00:00

  • Alleviating ischemia-reperfusion injury in aged rat liver by induction of heme oxygenase-1.

    abstract:BACKGROUND:Heme oxygenase-1 (HO-1), a cytoprotective protein, may be important in ameliorating hepatic ischemia-reperfusion (I/R) injury, a critical factor in the dysfunction of the aged liver after transplantation. METHODS:We used hemin to overexpress HO-1 and analyze its effects in a model of I/R in aged livers used...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Wang XH,Wang K,Zhang F,Li XC,Qian XF,Cheng F,Li GQ,Fan Y

    更新日期:2004-12-01 00:00:00

  • Hepatitis C and the incidence of diabetes mellitus after renal transplant: influence of new immunosuppression protocols.

    abstract:BACKGROUND:Hepatitis C has been associated with an increased incidence of diabetes mellitus (DM) following renal transplantation (RT). METHODS:Patients who underwent RT between 1985 and 2001 were excluded if they showed DM prior to RT, graft survival of less than 90 days, and unknown anti-HCV status (n=15). Two groups...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Gentil MA,López M,González-Roncero F,Rodríguez-Algarra G,Pereira P,López R,Martínez M,Toro J,Mateos J

    更新日期:2003-08-01 00:00:00

  • Adult endothelial progenitor cells retain hematopoiesis potential.

    abstract::Hematopoietic stem cells (HSCs) are derived from endothelium in the aortic-gonado-mesonephric (AGM) region during embryogenesis. But little is known about whether endothelial progenitor cells (EPCs) retain hematopoiesis potential after birth. In this study, we isolated adult EPCs from the bone marrow of C57BL/6 mice a...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Chen C,Zeng L,Ding S,Xu K

    更新日期:2010-11-01 00:00:00