The influence of hypoxia and acidity on the hyperthermic response of malignant cells in vitro.

Abstract:

:Colony formation of JB-1-E tumor cells was studied after hyperthermic treatment (42.5 degrees C) at a pH of 6.4 or 7.2 under hypoxic and euoxic conditions. At a pH of 7.2 and normal oxygen tension, there was a moderate decrease in colony formation with increasing duration of hyperthermic treatment (To = 65 min.). This effect was slightly enhanced under hypoxic conditions (To = 36 min.). The hyperthermic effect was enhanced to a considerably greater degree when treatment was performed at a pH of 6.4 (To = 19 min.), with no observable difference between hypoxia and euoxia. These findings indicate that environmental acidity is a determining factor in the hyperthermic effect. The hypoxic effect at a pH of 7.2 is probably due to a slight decrease in the intracellular pH caused by increased production of lactic acid.

journal_name

Radiology

journal_title

Radiology

authors

Overgaard J,Bichel P

doi

10.1148/123.2.511

subject

Has Abstract

pub_date

1977-05-01 00:00:00

pages

511-4

issue

2

eissn

0033-8419

issn

1527-1315

journal_volume

123

pub_type

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