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Transduction of human islets with the lentiviral vector.

Abstract：

:Establishment of an efficient gene delivery system for human pancreatic beta cells is important for the development of diabetes-targeted cell therapies. The human immunodeficiency virus type 1 (HIV-1)-derived lentiviral vector is well documented to be an effective gene transfer tool for various types of cells. Thus, we examined the efficiency of lentivirus-mediated gene delivery for human islets. Human islets were isolated using defined protocols for enzymatic dissociation and purification using discontinuous Ficoll gradients with a refrigerated Cobe 2991 machine. Isolated islets were shipped to Japan, cryopreserved for 3 months, and then subjected to transduction. A vesicular stomatitis virus G-protein (VSV-G)-pseudotyped lentiviral vector LtV-NLS/LacZ was produced in 293T cells under the Fugene6 method. 804G extracellular matrices were applied for monolayer formation of islets. Detection of NLS/LacZ expression was performed using X-gal staining. Lentiviral transduction was effective in these monolayer islets.

journal_name

Transplant Proc

journal_title

Transplantation proceedings

authors

Kobayashi N,Arata T,Okitsu T,Ikeda H,Kobayashi K,Kosaka Y,Narushima M,Tanaka N,Lakey JR

doi

10.1016/j.transproceed.2004.08.140

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

2203-4

issue

7

eissn

0041-1345

issn

1873-2623

pii

S0041-1345(04)01010-3

journal_volume

36

pub_type

杂志文章

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