Abstract:
:A rapid approach for estimating the pK(a) value of small organic molecules was developed using vacuum-assisted multiplexed capillary electrophoresis (VAMCE) with ultraviolet detection. The VAMCE method employed a 96-capillary array, arranged in a standard 8 x 12 microtiter plate configuration, with each row of capillaries filled with 12 individual running buffers of equal ionic strength (I = 50 mM) covering a pH range from 2.2 to 10.7. A separate compound was injected hydrodynamically into each row of capillaries allowing the estimation of pK(a) values for eight compounds in a single run. The application of a vacuum during the separation generated a bulk fluid flow and allowed the electrophoretic separation to be completed within 5 min. The complete VAMCE method, conditioning, and electrophoretic separation was optimized to allow the pK(a) estimation for between 128 to 168 compounds in an 8-h period. The VAMCE method provided a reliable approach for estimating pK(a) values both within- and between-day. The pK(a) values for a series of 96 compounds estimated by VAMCE agreed well with some of literature pK(a) values with an average absolute difference of 0.22 log units.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Zhou C,Jin Y,Kenseth JR,Stella M,Wehmeyer KR,Heineman WRdoi
10.1002/jps.20275subject
Has Abstractpub_date
2005-03-01 00:00:00pages
576-89issue
3eissn
0022-3549issn
1520-6017pii
S0022-3549(16)31729-4journal_volume
94pub_type
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