Abstract:
BACKGROUND:There is a very strong documented correlation between the appearance of cancer cells in blood and occurrence of metastasis in gastrointestinal cancer. AIM:To determine MUC1, CK19, CK20 and CEA mRNA expression in bone marrow of patients with gallbladder cancer and evaluate its clinical significance. MATERIAL AND METHODS:Sixty eight samples were analyzed, 38 bone marrow samples of gallbladder cancer patients, 20 healthy donors, and 10 frozen samples of gallbladder cancer. Nested reverse transcriptase-polymerase chain reaction (nested RT-PCR) was used to analyze mRNA expression. RESULTS:All frozen tumors were positive for CEA, CK19, and MUC1 mRNA and 70% were positive for CK20. Seventeen of 20 donor samples were positive for MUC1 and only one sample from donors was positive for both CK20 and CK19 mRNA. Among the 38 blood and bone marrow samples of gallbladder cancer patients, the expression of MUC1, CK19, CK20, and CEA, mRNA was 60.5% (23/38), 31.6% (12/38), 7.9% (3/38), and 7.9% (3/38), respectively. Disregarding the MUC1 results. 37% (14/38), 13% (5/38) and 5% (2/38) were positive for one, two and three markers respectively. Not significant differences were found in survival with a follow up to 12 months. CONCLUSION:Our results indicate that the molecular detection of tumor cells in bone marrow in patients with gallbladder carcinoma is technically possible, being CEA, CK19 and CK20 gene expression the best markers. The MUC1 gene expression marker was highly unspecific and it should not been considered. The detection of bone marrow micrometastasis might be helpful in prognosis and the selection of clinical treatment but a larger series with a longer follow-up should be studied.
journal_name
Rev Med Chiljournal_title
Revista medica de Chileauthors
Roa JC,Aretxabala Xd,Melo A,Faría G,Araya JC,Villaseca MA,Guzmán P,Roa Isubject
Has Abstractpub_date
2004-12-01 00:00:00pages
1489-98issue
12eissn
0034-9887issn
0717-6163pii
S0034-98872004001200006journal_volume
132pub_type
杂志文章abstract:BACKGROUND:Teaching practice is one of the most complex topics of the training process in medicine and other health care careers. The Teaching Practices Questionnaire (TPQ) evaluates teaching skills. AIM:To assess the factor structure and internal consistency of the Spanish version of the TPP among health care teacher...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872016000600015
更新日期:2016-06-01 00:00:00
abstract::We report a 35 years old female presenting with a right wrist pain. Physical examination was normal but, on a hand X ray examination, multiple dense para-articular bone foci were observed. Studying the rest of the skeleton, similar alterations were found in pelvis, knees, ankles, feet, shoulders and elbows. Osteopoiki...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::Aseptic meningitis after lumbar puncture and spinal anesthesia is a rare but serious complication, whose acute onset and clinical symptoms mimic septic meningitis. A 33 year old woman presented this complication 5 h after an uneventful cesarean section under subarachnoid blockade. Though lumbar puncture revealed pleoc...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::We investigated medical leaves of personnel at Temuco's hospital from 1986 to 1987. 57% of persons had at least one leave and 54% of these had 2 or more leaves. Women and paramedicals had more medical leaves. Most absences occurred in june, july, august (p less than 0.01). Most leaves were caused by respiratory diseas...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::The earth of ornamental plants is one of the main reservoirs of Aspergillus type of fungi in hospital areas. We studied 174 ornamental interior plants from a hospital at Santiago. Samples were obtained from the soil surface and sowed in Sabouraud-glucose agar, adding streptomycin and G-penicillin. After 72 h of cultur...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::Garengeot's hernia corresponds to the presence of the appendix within a femoral hernia, associated or not with acute appendicitis. The diagnosis of this uncommon situation is usually done during surgery. Furthermore, the clinical presentation as necrotizing fasciitis is a rare condition. We report a 54 years old obese...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872018000500660
更新日期:2018-05-01 00:00:00
abstract::The first and so far the only form of isolated growth hormone deficiency to accede to molecular genetic analysis is IGHD 1A, which has an autosomal recessive mode of inheritance. Several sizes of growth hormone gene deletions, ranging from 6.7 to 8.5 Kb are responsible for IGHD 1A. It has been reported that children w...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::The content and bioavailability of iron contained in Chilean bread was studied in a random sample from 301 bakeries. A wet digestion procedure followed by a colorimetric method was used. Iron bioavailability was examined in 9 adult women using the extrinsic radioactive tag method. The iron content of bread was 2.4 +/-...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::The aim of this article is to analyze the current situation of AIDS in Latin American countries. Specifically, to compare their situation with other geographic areas of the planet, the prevalence index per countries, distribution per sex and age and, finally, the main ways of transmission in each country. The most rec...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:
更新日期:2001-08-01 00:00:00
abstract::Polycystic ovarian syndrome (PCOS) is an endocrine and metabolic dysfunction, highly prevalent in women in their reproductive years. Hyperandrogenism, oligo-ovulation, polycystic ovarian morphology are the main features of this syndrome. PCOS is a genetic disorder with a multifactorial etiology and has a strong link w...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:10.4067/s0034-98872017000700907
更新日期:2017-07-01 00:00:00
abstract::Glucose toxicity refers to the structural and functional damage in the beta cells and target tissues of insulin, caused by chronic hyperglycemia. These alterations cause a lower hormonal secretion and action (insulin resistance). Lipid toxicity refers to the damage caused by persistently high free fatty acid levels, a...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:
更新日期:2001-06-01 00:00:00
abstract:BACKGROUND:There is a wide interindividual variability in the response to a period of exercise training. The science have reported that a minimum of participants could be non-responders for improving different health-related outcomes after training. AIM:To compare the effects of a 6-weeks exercise program on body comp...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872018000600693
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Demands on primary health care services in Chile are increasing. The staff perception of specific sources of tension at work is not known. This knowledge is necessary to avoid preventable health problems caused by distress. AIM:To identify specific work stressors in primary health care facilities and to exa...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872006000400007
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:The etiology of acute exacerbations of chronic obstructive pulmonary disease (COPD) is heterogeneous and still under discussion. Inflammation increases during exacerbation of COPD. The identification of inflammatory changes will increase our knowledge and potentially guide therapy. AIM:To identify which inf...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872012000100002
更新日期:2012-01-01 00:00:00
abstract::Accreditation has acquired special relevance for the habilitation of professional practice, considering the proliferation of teaching institutions, the variety of training programs and the increasing amount of professionals requiring accreditation or revalidation of their titles. The internationalization of profession...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:Genetically programmed adult-type hypolactasia affects 56% of Chilean population. Ideally, diagnosis should be confirmed. AIM:To compare diagnostic yield of genetic test, hydrogen (H2) expiratory test and a validated symptomatic structured survey (SS). MATERIAL AND METHODS:Patients submitted to H2 test ans...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872012000900001
更新日期:2012-09-01 00:00:00
abstract::We compared 2 techniques for determination of plasma levels of phenytoin, liquid-gas chromatography (LGC) and homogeneous enzymatic immunoassay (EI) in samples from 72 patients receiving monotherapy with this drug. Determinations were also made in samples of normal plasma with known concentrations of phenytoin. Good c...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::Environmental factors may influence the development of Inflammatory Bowel Disease and modify its natural history. The objective of this review is to evaluate current evidence about environmental factors associated with the disease. A better knowledge about the pathogenesis of the disease can lead to better treatment s...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:10.4067/s0034-98872019000200212
更新日期:2019-02-01 00:00:00
abstract::This article deals with the history and evolution of student's scientific journals in Latin-America, their beginnings, how many still exist and which is their future projection. Relevant events show the growth of student's scientific journals in Latin-America and how are they working together to improve their quality....
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872010001200016
更新日期:2010-11-01 00:00:00
abstract:UNLABELLED:Circulating concentrations of the high affinity growth hormone binding protein (GHBP) may be a marker of GH receptor density as well as GH sensitivity. GOAL:To determine values of GHBP for a normal Chilean pediatric population. METHODS:We determined GHBP levels in 73 males and 73 females between 4 to 15.5 ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::The aim of this study was to assess the predictive value for mortality of admission and daily APACHE II score, mortality due to multiple organ failure and the Organ Failure Score in patients with acute hepatic failure. We retrospectively studied 15 such patients admitted to an intensive care unit. Thirteen patients di...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::Kidney graft loss because arterial thrombosis is not common and is related to risk factors such as recurrent vascular hemodialysis access thrombosis, collagen-vascular disease, repeated miscarriage, diabetes mellitus and thrombophilia. Patients having this last disorder have an increased risk of repeated thrombosis in...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872007000100014
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Cardiac rehabilitation (CR) programs play an important role in the control and prevention of new cardiac events. AIM:A survey was performed to evaluate the current situation of CR programs in Chile. MATERIAL AND METHODS:A questionnaire evaluating the structure of rehabilitation centers, characteristics of ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872012000500002
更新日期:2012-05-01 00:00:00
abstract::This article reviews some key issues of early rheumatoid arthritis such as the dfficulties to recognize this condition during the first months after onset. Therefore, three reference diagnostic criteria have been proposed for any patient presenting with more than three simultaneously inflammed joints, involvement of m...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:10.4067/s0034-98872008001100015
更新日期:2008-11-01 00:00:00
abstract::Normal myocardial cells actively uptake the radioisotope Thallium-201 and its myocardial distribution is a reflection of the existent regional coronary artery blood flow at the moment of the examination. Thallium-201 reaches quickly a high intracellular concentration and then leaves myocardial cells in a mean time of ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Obesity has a high prevalence among children. On the other hand, acute respiratory infections especially of viral origin, are an important cause of morbidity and mortality in this age group. During the recent pandemic of influenza A (H1N1) virus, obesity was identified as a novel independent risk factor for severity m...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:10.4067/S0034-98872016000900012
更新日期:2016-09-01 00:00:00
abstract::We evaluated the concentration of cefotaxime in the exudate from ischemic leg ulcers in 9 subjects with severe obstructive arterial disease. The administration of the drug was either systemic (1 g in 250 ml of saline given in 30 min) or regional at a vein of the foot while a tourniquet was applied at the level of the ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::Laugier-Hunziker syndrome is a rare benign idiopathic condition characterized by acquired macular pigmentation of lips and buccal mucosa, often accompanied with melanonychia. The main concern with this condition is to rule out other differential diagnosis with systemic repercussions and similar hyperpigmentation patte...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872016000500017
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:The six minute walk test (6MWT) is an important physical performance measure used in older people. AIM:To assess the total distance walked and the physiological cost of the 6MWT in non-disabled older people. MATERIAL AND METHODS:Fifty six females aged 69 ± 5 years with a body mass index (BMI) of 31 ± 4 kg/...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872015000400010
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:Recent studies in mice have demonstrated that the Msx-1 homebox gene is implicated in cleft palate. Thus, it has been suggested that its human homologue, MSX1 (HOX-7), located in chromosome 4 could be involved in the etiology of non syndromic cleft lip palate. AIM:To study the linkage between non syndromic ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00