Abstract:
:In this report we characterized the transcriptional regulation of the rat mdr1b gene by xenobiotics. The expression of this gene was increased in primary rat hepatocytes and in the H4-II-E hepatoma cell line by exposure to carcinogens such as aflatoxin B1, N-acetoxy-2-acetylaminofluorene, and methyl methanesulfonate. Nuclear run-on experiments indicated that the higher steady-state levels of mdr1b mRNA were due to an increase in transcription. The 5'-flanking region of the mdr1b gene was isolated, sequenced, and functionally characterized in transient and stable transfection assays. A single transcription start site was identified for this gene; no alternate start sites were used after induction with aflatoxin B1. Deletion analysis of this promoter demonstrated that the sequence between nt -214 and -178 was critical for basal promoter activity. This region did not contain any consensus-binding sites for previously identified transcription factors. A negative regulatory region was also identified between nt -940 and -250. No specific carcinogen-responsive element was identified; the xenobiotic response required a large part of the promoter. These data suggest that the carcinogen induction of mdr1b expression is mediated through sequences that overlap or that are identical to the basal promoter element.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Silverman JA,Hill BAdoi
10.1002/mc.2940130109subject
Has Abstractpub_date
1995-05-01 00:00:00pages
50-9issue
1eissn
0899-1987issn
1098-2744journal_volume
13pub_type
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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journal_title:Molecular carcinogenesis
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-10-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940100404
更新日期:1994-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.22321
更新日期:2016-05-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22031
更新日期:2014-02-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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更新日期:2017-03-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20545
更新日期:2009-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.22158
更新日期:2015-09-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940020403
更新日期:1989-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940120205
更新日期:1995-02-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20250
更新日期:2008-02-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20670
更新日期:2010-10-01 00:00:00