Contractile properties of the developing fetal sheep bladder.

Abstract:

AIM:To characterise the developmental changes to the normal bladder by examining the in vitro contractile properties of the fetal sheep detrusor smooth muscle bladder at different gestational ages. METHODS:Three groups of normally developing fetal sheep bladders were investigated: (1) 65-70 days gestation (2nd trimester); (2) 105-110 days (3rd trimester); (3) 135-140 days (term). Isometric contractions in isolated detrusor strips were measured during either electrical field stimulation (EFS) or exposure to agonists: carbachol, alpha-beta methylene-ATP (ABMA) and KCl. RESULTS:There was a significant increase of absolute force elicited by EFS with fetal age. Contractures elicited by carbachol, ABMA and KCl increased between Groups 1 and 2, but not thereafter. The proportional increase of contractions elicited by carbachol and ABMA was also greater between Groups 1 and 2, than for EFS and KCl. CONCLUSIONS:Fetal development between 65 and 140 days in the sheep is associated with increased contractile activation. The data are consistent with an increase of muscle development in the earlier stages (65-110 days). In the later stage (110-140 days) muscle development is complete but functional innervation of the tissue continues.

journal_name

Neurourol Urodyn

authors

Nyirády P,Thiruchelvam N,Godley ML,David A,Cuckow PM,Fry CH

doi

10.1002/nau.20124

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

276-81

issue

3

eissn

0733-2467

issn

1520-6777

journal_volume

24

pub_type

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