Suppressive effects of TNF-alpha on myelin formation in vitro.

Abstract:

:Using organotypic cultures of ICR mouse cerebellum, we studied the effects of TNF-alpha on myelin formation, and its demyelinating effects. Mature, myelinated cultures were resistant to TNF-alpha, even at the highest concentration examined, 1 x 10(4) U/ml. However, when cultures were grown in medium containing TNF-alpha from the time of explantation, myelin formation was significantly affected at a concentration of 1 x 10(2) U/ml. The myelin sheaths formed were very thin, partial, and few in number. When TNF-alpha was removed from the medium within 6 days, myelin formation was recovered. Although our in vitro system tests factors that influence myelin formation but not remyelination, these results suggest that TNF-alpha may be involved in the remyelination block following acute myelin breakdown.

journal_name

Acta Neurol Scand

authors

Tsukamoto T,Ishikawa M,Yamamoto T

doi

10.1111/j.1600-0404.1995.tb05847.x

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

71-5

issue

1

eissn

0001-6314

issn

1600-0404

journal_volume

91

pub_type

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