Translational regulation of human beta interferon mRNA: association of the 3' AU-rich sequence with the poly(A) tail reduces translation efficiency in vitro.

abstract：:Nonsense-mediated RNA decay (NMD) is an mRNA surveillance mechanism which rapidly degrades select cytoplasmic mRNAs. We and others have shown that NMD is a dynamically regulated process inhibited by amino acid deprivation, hypoxia, and other cellular stresses commonly generated by the tumor microenvironment. This inhi...

journal_title：Molecular and cellular biology

authors： Wengrod J,Martin L,Wang D,Frischmeyer-Guerrerio P,Dietz HC,Gardner LB

更新日期：2013-06-01 00:00:00

abstract：:During Xenopus development, the synthesis of ribosomal proteins is regulated at the translational level. To identify the region of the ribosomal protein mRNAs responsible for their typical translational behavior, we constructed a fused gene in which the upstream sequences (promoter) and the 5' untranslated sequence (f...

journal_title：Molecular and cellular biology

authors： Mariottini P,Amaldi F

更新日期：1990-02-01 00:00:00

abstract：:We show that the nuclear genes for the cytoplasmic and mitochondrial leucyl-tRNA synthetase (LeuRS) of Neurospora crassa are distinct in their encoded proteins, codon usage, mRNA levels, and regulation. The 4.2-kilobase-pair region representing the structural gene for cytoplasmic LeuRS and flanking regions has been se...

journal_title：Molecular and cellular biology

authors： Chow CM,Rajbhandary UL

更新日期：1989-11-01 00:00:00

abstract：:A protein with specific affinity for the mRNA cap structure was purified both from the postribosomal supernatant and from the ribosomal high-salt wash of Drosophila melanogaster embryos by m7GTP-Sepharose chromatography. This protein had an apparent molecular mass of 35 kilodaltons (kDa) in sodium dodecyl sulfate-poly...

journal_title：Molecular and cellular biology

authors： Maroto FG,Sierra JM

更新日期：1989-05-01 00:00:00

abstract：:Major histocompatibility complex class II (MHCII) molecules play a pivotal role in the immune system because they direct the development and activation of CD4(+) T cells. There are three human MHCII isotypes, HLA-DR, HLA-DQ, and HLA-DP. Key transcription factors controlling MHCII genes have been identified by virtue o...

journal_title：Molecular and cellular biology

authors： Peretti M,Villard J,Barras E,Zufferey M,Reith W

更新日期：2001-09-01 00:00:00

abstract：:In mast cells, antigen-mediated aggregation of the high-affinity receptor for immunoglobulin E, Fc epsilon RI, stimulates tyrosine phosphorylation and activation of multiple signaling pathways leading to the release of several classes of mediators of the allergic response. Early events induced upon cross-linking of Fc...

journal_title：Molecular and cellular biology

authors： Rivera VM,Brugge JS

更新日期：1995-03-01 00:00:00

abstract：:Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit m...

journal_title：Molecular and cellular biology

authors： Nielsen J,Christiansen J,Lykke-Andersen J,Johnsen AH,Wewer UM,Nielsen FC

更新日期：1999-02-01 00:00:00

abstract：:CD133, a widely known marker of cancer stem cells, was recently found in extracellular vesicles. However, the mechanisms underlying CD133 translocation to the extracellular space remain largely unknown. Here we report that CD133 is monoubiquitinated. Ubiquitination occurs primarily on complex glycosylated CD133. The l...

journal_title：Molecular and cellular biology

authors： Yang F,Xing Y,Li Y,Chen X,Jiang J,Ai Z,Wei Y

更新日期：2018-07-16 00:00:00

abstract：:Alternative mRNA splicing is an important means of diversifying function in higher eukaryotes. Notably, both NCoR and SMRT corepressors are subject to alternative mRNA splicing, yielding a series of distinct corepressor variants with highly divergent functions. Normal adipogenesis is associated with a switch in corepr...

journal_title：Molecular and cellular biology

authors： Goodson ML,Young BM,Snyder CA,Schroeder AC,Privalsky ML

更新日期：2014-11-15 00:00:00

abstract：:Saccharomyces cerevisiae dbf4 and cdc7 cell cycle mutants block initiation of DNA synthesis (i.e., are iDS mutants) at 37 degrees C and arrest the cell cycle with a 1C DNA content. Surprisingly, certain dbf4 and cdc7 strains divide their chromatin at 37 degrees C. We found that the activation of the Cdc28 mitotic prot...

journal_title：Molecular and cellular biology

authors： Toyn JH,Johnson AL,Johnston LH

更新日期：1995-10-01 00:00:00

abstract：:Increasing evidence supports an important biological role for Myc in the downregulation of specific gene transcription. Recent studies suggest that c-Myc may suppress promoter activity through proteins of the basal transcription machinery. We have previously reported that Myc protein, in combination with additional ce...

journal_title：Molecular and cellular biology

authors： Facchini LM,Chen S,Marhin WW,Lear JN,Penn LZ

更新日期：1997-01-01 00:00:00

abstract：:CTCFL, a paralog of CTCF, also known as BORIS (brother of regulator of imprinted sites), is a testis-expressed gene whose function is largely unknown. Its product is a cancer testis antigen (CTA), and it is often expressed in tumor cells and also seen in two benign human vascular malformations, juvenile angiofibromas ...

journal_title：Molecular and cellular biology

authors： Sati L,Zeiss C,Yekkala K,Demir R,McGrath J

更新日期：2015-10-01 00:00:00

abstract：:Fms is the receptor for macrophage colony-stimulating factor (M-CSF) and contains intrinsic tyrosine kinase activity. Expression of exogenous Fms in a murine myeloid progenitor cell line, FDC-P1 (FD-Fms), results in M-CSF-dependent growth and macrophage differentiation. Previously, we described a 100-kDa protein that ...

journal_title：Molecular and cellular biology

authors： Liu Y,Jenkins B,Shin JL,Rohrschneider LR

更新日期：2001-05-01 00:00:00

abstract：:To understand the mechanisms by which the chromatin-remodeling SWI/SNF complex interacts with DNA and alters nucleosome organization, we have imaged the SWI/SNF complex with both naked DNA and nucleosomal arrays by using energy-filtered microscopy. By making ATP-independent contacts with DNA at multiple sites on its s...

journal_title：Molecular and cellular biology

authors： Bazett-Jones DP,Côté J,Landel CC,Peterson CL,Workman JL

更新日期：1999-02-01 00:00:00

abstract：:Calcineurin, or PP2B, plays a critical role in mediating Ca2+-dependent signaling in many cell types. In yeast cells, this highly conserved protein phosphatase regulates aspects of ion homeostasis and cell wall synthesis. We show that calcineurin mutants are sensitive to high concentrations of Mn2+ and identify two ge...

journal_title：Molecular and cellular biology

authors： Pozos TC,Sekler I,Cyert MS

更新日期：1996-07-01 00:00:00

abstract：:The Wnt signaling pathway plays a pivotal role in vertebrate early development and morphogenesis. Duplin (axis duplication inhibitor) interacts with beta-catenin and prevents its binding to Tcf, thereby inhibiting downstream Wnt signaling. Here we show that Duplin is expressed predominantly from early- to mid-stage mo...

journal_title：Molecular and cellular biology

authors： Nishiyama M,Nakayama K,Tsunematsu R,Tsukiyama T,Kikuchi A,Nakayama KI

更新日期：2004-10-01 00:00:00

abstract：:The CBP/p300 family of proteins comprises related acetyltransferases that coactivate signal-responsive transcription. Recent evidence suggests that p300/CBP may also interact directly with complexes that mediate different aspects of DNA metabolism such as replication and repair. In this report, we show that loss of dC...

journal_title：Molecular and cellular biology

authors： Smolik S,Jones K

更新日期：2007-01-01 00:00:00

abstract：:Mutations in the gene encoding methyl-CpG-binding protein 2 (MeCP2) lead to disrupted neuronal function and can cause the neurodevelopmental disorder Rett syndrome. MeCP2 is a transcriptional regulator that binds to methylated DNA and is most abundant in neuronal nuclei. The mechanisms by which MeCP2 regulates gene ex...

journal_title：Molecular and cellular biology

authors： Gonzales ML,Adams S,Dunaway KW,LaSalle JM

更新日期：2012-07-01 00:00:00

abstract：:The mouse urokinase-type plasminogen activator (uPA) gene was used as a model macrophage colony-stimulating factor 1 (CSF-1)-inducible gene to investigate CSF-1 signalling pathways. Nuclear run-on analysis showed that induction of uPA mRNA by CSF-1 and phorbol myristate acetate (PMA) was at the transcriptional level i...

journal_title：Molecular and cellular biology

authors： Stacey KJ,Fowles LF,Colman MS,Ostrowski MC,Hume DA

更新日期：1995-06-01 00:00:00

abstract：:In mammals, molecular mechanisms and factors involved in the tight regulation of telomerase expression and activity are still largely undefined. In this study, we provide evidence for a role of estrogens and their receptors in the transcriptional regulation of hTERT, the catalytic subunit of human telomerase and, cons...

journal_title：Molecular and cellular biology

authors： Misiti S,Nanni S,Fontemaggi G,Cong YS,Wen J,Hirte HW,Piaggio G,Sacchi A,Pontecorvi A,Bacchetti S,Farsetti A

更新日期：2000-06-01 00:00:00

abstract：:Retinoid X receptors (RXR) have been identified as common subunits in the regulation of multiple hormonal signaling pathways. Using circular permutation and phasing analysis of specific response elements, we present evidence that RXR-retinoic acid receptor and RXR-thyroid hormone receptor heterodimer or RXR-RXR homodi...

journal_title：Molecular and cellular biology

authors： Lu XP,Eberhardt NL,Pfahl M

更新日期：1993-10-01 00:00:00

abstract：:Drosophila virilis genomic DNA corresponding to the D. melanogaster embryonic lethal abnormal visual system (elav) locus was cloned. DNA sequence analysis of a 3.8-kb genomic piece allowed identification of (i) an open reading frame (ORF) with striking homology to the previously identified D. melanogaster ORF and (ii)...

journal_title：Molecular and cellular biology

authors： Yao KM,White K

更新日期：1991-06-01 00:00:00

abstract：:The LAR family protein tyrosine phosphatases (PTPs), including LAR, PTP delta, and PTP sigma, are transmembrane proteins composed of a cell adhesion molecule-like ectodomain and two cytoplasmic catalytic domains: active D1 and inactive D2. We performed a yeast two-hybrid screen with the first catalytic domain of PTP s...

journal_title：Molecular and cellular biology

authors： Wallace MJ,Fladd C,Batt J,Rotin D

更新日期：1998-05-01 00:00:00

abstract：:As part of an effort to characterize the spatial and functional relationships among genetic elements within the amplified dihydrofolate reductase (DHFR) domain in Chinese hamster cells, we have used a variation of the differential hybridization approach to identify cDNA clones whose genes are coamplified with DHFR in ...

journal_title：Molecular and cellular biology

authors： Foreman PK,Hamlin JL

更新日期：1989-03-01 00:00:00

abstract：:Nearly 40% of cases of acute myelogenous leukemia (AML) of the M2 subtype are due to a chromosomal translocation that combines a sequence-specific DNA binding protein, AML1, with a potent transcriptional repressor, ETO. ETO interacts with nuclear receptor corepressors SMRT and N-CoR, which recruit histone deacetylase ...

journal_title：Molecular and cellular biology

authors： Zhang J,Hug BA,Huang EY,Chen CW,Gelmetti V,Maccarana M,Minucci S,Pelicci PG,Lazar MA

更新日期：2001-01-01 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) is controlled by cellular signaling pathways that trigger the loss of cell-cell adhesion and lead to the restructuring of the cell cytoskeleton. Transforming growth factor β (TGF-β) has been shown to regulate cell plasticity through the phosphorylation of Par6 on a conserved ...

journal_title：Molecular and cellular biology

authors： Gunaratne A,Thai BL,Di Guglielmo GM

更新日期：2013-03-01 00:00:00

abstract：:We investigated the recognition of the conserved 5-bp repeated motif NGAAN, which occurs in heat shock gene promoters of Drosophila melanogaster and other eukaryotic organisms, by human heat shock transcription factor (HSF). Extended heat shock element mutants of the human HSP70 gene promoter, containing additional NG...

journal_title：Molecular and cellular biology

authors： Cunniff NF,Wagner J,Morgan WD

更新日期：1991-07-01 00:00:00

abstract：:Bright/Arid3a has been characterized both as an activator of immunoglobulin heavy-chain transcription and as a proto-oncogene. Although Bright expression is highly B lineage stage restricted in adult mice, its expression in the earliest identifiable hematopoietic stem cell (HSC) population suggests that Bright might h...

journal_title：Molecular and cellular biology

authors： Webb CF,Bryant J,Popowski M,Allred L,Kim D,Harriss J,Schmidt C,Miner CA,Rose K,Cheng HL,Griffin C,Tucker PW

更新日期：2011-03-01 00:00:00

abstract：:The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a del...

journal_title：Molecular and cellular biology

authors： Schiestl RH,Prakash S

更新日期：1990-06-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) accumulates in virus-infected mammalian cells and signals the activation of host defense pathways of the interferon system. We describe here a novel form of dsRNA-triggered signaling that leads to the stimulation of the p38 mitogen-activated protein kinase (p38 MAPK) and the c-Jun NH(2)-ter...

journal_title：Molecular and cellular biology

authors： Iordanov MS,Paranjape JM,Zhou A,Wong J,Williams BR,Meurs EF,Silverman RH,Magun BE

更新日期：2000-01-01 00:00:00