Abstract:
:To understand the mechanisms by which the chromatin-remodeling SWI/SNF complex interacts with DNA and alters nucleosome organization, we have imaged the SWI/SNF complex with both naked DNA and nucleosomal arrays by using energy-filtered microscopy. By making ATP-independent contacts with DNA at multiple sites on its surface, SWI/SNF creates loops, bringing otherwise-distant sites into close proximity. In the presence of ATP, SWI/SNF action leads to the disruption of nucleosomes within domains that appear to be topologically constrained by the complex. The data indicate that the action of one SWI/SNF complex on an array of nucleosomes can lead to the formation of a region where multiple nucleosomes are disrupted. Importantly, nucleosome disruption by SWI/SNF results in a loss of DNA content from the nucleosomes. This indicates a mechanism by which SWI/SNF unwraps part of the nucleosomal DNA.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Bazett-Jones DP,Côté J,Landel CC,Peterson CL,Workman JLdoi
10.1128/mcb.19.2.1470subject
Has Abstractpub_date
1999-02-01 00:00:00pages
1470-8issue
2eissn
0270-7306issn
1098-5549journal_volume
19pub_type
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