Abstract:
:Angiogenesis was examined by a three-dimensional model in vitro, using human umbilical vein endothelial cells (HUVECs) cultured in a collagen gel. An abundant capillary-like network with a lumen structure was identified histologically and shown to have formed at a collagen density of 0.05% or 0.10% instead of 0.15%, for either type I or type III collagen. At the same density, type III collagen induced a capillary-like network with HUVECs at an earlier stage of culture than type I collagen. In a two-dimensional culture, HUVECs were viable and proliferated to a great extent on the culture dish coated with collagen. This was particularly so in the case of type III collagen at lower density (5 ng/cm2). Type III collagen at a sufficiently low density is thus shown useful for studying angiogenesis in vitro. The capillary-like network that formed in the three-dimensional culture appeared somewhat labile, but became stable with the continuous addition of endothelial cell growth supplement and increase in the plating number of HUVECs.
journal_name
Ann Plast Surgjournal_title
Annals of plastic surgeryauthors
Kanzawa S,Endo H,Shioya Ndoi
10.1097/00000637-199303000-00008subject
Has Abstractpub_date
1993-03-01 00:00:00pages
244-51issue
3eissn
0148-7043issn
1536-3708journal_volume
30pub_type
杂志文章abstract:BACKGROUND:Tissue expander and implant remains the most common technique for breast reconstruction. A controversial topic within this method is routine use of acellular dermal matrix (ADM). Acellular dermal matrices have increased risks of infection, seroma, hematoma, skin flap necrosis, and total complications. METHO...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000001322
更新日期:2018-06-01 00:00:00
abstract::Excessive scar contracture by wound fibroblasts can have devastating consequences, ranging from body disfigurement to joint immobility. The ability of fibroblasts isolated from lesions of hypertrophic scars, keloids, normal skin, or normal scars in contracting the provisional wound matrix (i.e., fibrin clot) was compa...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199605000-00011
更新日期:1996-05-01 00:00:00
abstract::A randomized, prospective, double-blind study designed to compare the effectiveness of the use of cloxacillin with the result of nontreatment in patients undergoing surgical intervention of axillary hyperhidrosis was conducted from August 1987 to October 1987 at the Veterans General Hospital, Taipei, Taiwan. Forty-two...
journal_title:Annals of plastic surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00000637-198905000-00009
更新日期:1989-05-01 00:00:00
abstract::In the small percentage of deep wound infections complicating total hip arthroplasty and recalcitrant to conventional forms of treatment, muscle flaps with or without a cutaneous unit can offer a satisfactory solution. Seven patients (4 male, 3 female) with failed total hip arthroplasty and multimicrobial infection we...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198312000-00006
更新日期:1983-12-01 00:00:00
abstract::Craniofacial surgical techniques have yielded better methods of treating severe craniofacial trauma. In this paper surgical techniques are discussed in principle and specifically presented through case reports. We conclude that the most important factors for successful reconstruction after craniofacial trauma are to d...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198612000-00012
更新日期:1986-12-01 00:00:00
abstract::The authors present their successful experience with an attractive alternative to the latissimus dorsi and gracilis muscle flaps for lower extremity reconstruction--the free rectus abdominis muscle flap. The muscle flap has a dual blood supply, but the longer pedicle length and larger vessel diameter of the inferior e...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198311000-00003
更新日期:1983-11-01 00:00:00
abstract::We report the use of topic negative pressure for seromas resulting from mobilization of tensor fascia lata miocutaneous flap in the trochanteric areas for pressure sores. In 5 consecutive patients we successfully treated seroma with the use of external and internal topic subatmospheric pressure. ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31819adf94
更新日期:2009-12-01 00:00:00
abstract:BACKGROUND:Scalp melanoma is aggressive and has a proclivity for regional metastasis. We hypothesize that subperiosteal scalp melanoma resection reduces in-transit/satellite recurrence, when compared with subgaleal resection. METHODS:We identified patients with intermediate to deep, primary scalp melanoma referred to ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31822592e7
更新日期:2012-08-01 00:00:00
abstract::Nasolabial flaps are frequently used for reconstruction of nasal and midfacial defects. The donor site and the quality of the skin provided are excellent, though reconstructive results are sometimes hampered by a dog-ear at the base of the flap. Properly designed rhomboid flaps generally rotate into position without a...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199209000-00015
更新日期:1992-09-01 00:00:00
abstract::The aim of the study was to evaluate single-injection gamma probe-guided sentinel lymph node (SLN) detection, applied in 40 melanomatous selective sentinel lymphadenectomies (SSLNDs). Thirty-four patients underwent preoperative lymphoscintigraphy, intraoperative SLN identification by a gamma-detecting probe and blue d...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199810000-00008
更新日期:1998-10-01 00:00:00
abstract::Various techniques and modifications have been introduced in the treatment of mandibular prognathism. However, there are still few reports concerning long-term stability, especially using the intraoral vertical ramus osteotomy (IVRO) method. The purpose of this study was to investigate the long-term stability for corr...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e318153f3ee
更新日期:2008-07-01 00:00:00
abstract:INTRODUCTION:Our objective was to determine the compliance of observational studies in plastic surgery with the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement checklist. METHODS:All cohort, cross-sectional, and case-control studies published in five major plastic surgery journa...
journal_title:Annals of plastic surgery
pub_type: 杂志文章,评审
doi:10.1097/SAP.0000000000000419
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Obstetric brachial plexus palsy is caused by traction during birth. Most patients regain useful function with spontaneous recovery. In some cases, cross reinnervation occurs between the biceps and triceps muscles. In these cases, smooth active motion of the elbow joint is impaired by simultaneous biceps and ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000002075
更新日期:2019-10-01 00:00:00
abstract::The study of preserved porcine skin was done by using split-thickness skin grafts of pigs weighing approximately 25 to 30 kg. The harvesting of split-thickness skin grafts was performed under aseptic and antiseptic conditions. The povidone-iodine solution and 0.25% acidified sodium hypochlorite solution were used befo...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199304000-00009
更新日期:1993-04-01 00:00:00
abstract::The authors present the successful transfer of a compound flap based on the sternocleidomastoid muscle in a patient who had undergone radiation therapy and a near-total laryngectomy. The surgical procedure, a one-stage reconstruction with a regional rotational flap, utilized a segment of the sternal periosteum and und...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-200045020-00018
更新日期:2000-08-01 00:00:00
abstract:PURPOSE:The reconstruction of complicated defects with 3-dimensional deficits remains challenging. The reconstruction of these defects requires not only coverage of surface but also appropriate obliteration of dead space, and muscle-chimeric perforator flaps can be a valuable option. Here, we present our experience wit...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e3182a6363c
更新日期:2015-05-01 00:00:00
abstract::Hair transplantation involves the transplantation of hair, beard, eyebrows, eyelashes, and pubic hair. Based on our experience, the aesthetic result of hair transplantation mainly relies on 4 indicators, including selection of the donor site, direction and angle of grafted hairs, density, and survival rate of implante...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000000778
更新日期:2016-02-01 00:00:00
abstract::The present study describes the techniques available for single-stage sarcoma resection, soft-tissue reconstruction, and radiotherapy for limb preservation in patients who are unable to undergo primary wound closure after a complete soft-tissue resection of their primary sarcoma. From 1989 to 1994, 19 patients (age ra...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199711000-00003
更新日期:1997-11-01 00:00:00
abstract::The almost single disadvantage of polyurethane film dressings, an uncontrolled leakage, is probably as often described as its numerous advantages for split-thickness skin graft donor sites. We solved this problem by perforating the polyurethane film, which permits a controlled leakage into a secondary absorbent dressi...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31818790b4
更新日期:2009-08-01 00:00:00
abstract:INTRODUCTION:Use of the omentum for poststernotomy mediastinitis is typically viewed as a last resort. Formal debridement and muscle flap coverage is sufficient most of the time; however, there are situations when the omental flap is more appropriate. The purpose of this series is to critically evaluate the outcome in ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e318054718e
更新日期:2008-02-01 00:00:00
abstract::This article describes a model of chronic nerve compression in the rat. The sciatic nerve of adult male Sprague Dawley rats was banded with a 1-cm Silastic tube for varying periods of time. Morphometric analysis, electrodiagnostic studies, and histological evaluation were carried out 3, 5, 8, and 12 months after bandi...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198711000-00008
更新日期:1987-11-01 00:00:00
abstract::In fingertip amputations, conventional stump plasty provides an almost acceptable functional result. However, replanting fingertips can preserve the nail and minimize loss of function. We investigated the functional and cosmetic results of fingertip replantation at the terminal branch of the digital artery. Outcomes w...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/01.sap.0000137136.09890.18
更新日期:2004-10-01 00:00:00
abstract::Rats subjected to a standard electrical burn of 250 volts for 10 seconds receive a severe injury stimulating a pronounced systemic circulatory response. Initial postinjury hyperemia is replaced by a low perfusion state within 24 hours. Our study demonstrates the difficulty in isolating regional microcirculatory altera...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198602000-00007
更新日期:1986-02-01 00:00:00
abstract::A fasciocutaneous free graft could prove the ideal free tissue graft to prevent contracture following adhesion of the graft to the recipient bed. However, for graft survival, vascular anastomosis involving complicated surgical techniques is necessary. In the present study, we focused on peripheral blood-derived CD133(...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e3181934951
更新日期:2009-09-01 00:00:00
abstract::A rare congenital anomaly of the face has been encountered and reported: lateral nasal proboscis. It is characterized by a trunk-like tube arising from an eyelid and is coupled with hypoplasia of the nose, abnormal orbit and maxilla, and ophthalmic anomalies. The pathogenesis and treatment of this disorder are discuss...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract:INTRODUCTION:Plantar hyperkeratosis, such as corns and calluses, is common in older people and associated with pain, mobility impairment, and functional limitations. It usually develops on the palms, knees, or soles of feet, especially under the heels or balls. There are several treatment methods for plantar hyperkerat...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000001304
更新日期:2018-02-01 00:00:00
abstract::Our prospective clinical trial collected sensory data using a computerized pressure-specified sensory device comparing 4 procedures for reduction mammaplasty. A total of 48 patients were assessed at baseline, 6 weeks (n = 42), 6 months (n = 15), and 1 year (n = 24) postoperatively. The findings of our study showed pre...
journal_title:Annals of plastic surgery
pub_type: 临床试验,杂志文章
doi:10.1097/SAP.0b013e3182126854
更新日期:2012-02-01 00:00:00
abstract::The first reports on gender-confirming surgery had been published in Germany in the 1920s, but it took some 30 years before sex reassignment surgery for transsexuals became generally known. Initially, such surgery was performed mostly in Europe and Casablanca. In 1956, the gynecologist Dr Georges Burou (1910-1987) ind...
journal_title:Annals of plastic surgery
pub_type: 传,历史文章,杂志文章
doi:10.1097/01.sap.0000258974.41516.bc
更新日期:2007-12-01 00:00:00
abstract::The nasal reconstruction in 8 patients and cheek reconstruction in 1 using a free flap from the deltoid region has been successfully undertaken in our department since August 1987. The flap has a direct cutaneous artery--the acromial artery--as its vascular axis. The experiences and a brief anatomical review of the do...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198907000-00003
更新日期:1989-07-01 00:00:00
abstract::The "central" approach for single-stage debulking of the reverse sural artery fasciomusculocutaneous flap is described. A "central" incision is made along the longitudinal axis of the flap, and the skin is completely elevated up to the edges of the flap. The technique was used in 20 consecutive cases and primary wound...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/01.sap.0000252715.95110.56
更新日期:2007-08-01 00:00:00