Abstract:
:The control of metallothionein (MT) synthesis was investigated in freshly prepared rat hepatocytes in experiments of short-term duration. Viability and metabolic function were maintained in incubations of 6-h duration. MT synthesis was measurable in hepatocytes from fed rats at Zn concentrations down to 1 microM. Zn and dexamethasone induced concentration-dependent increases in the synthesis of MT with maximal increases above the 5-h control of 3.2- and 2.5-fold, respectively. Zn induction of MT was first measurable at 2 h and was inhibited by actinomycin C. Although initial (0 h) MT concentrations in hepatocytes from fasted rats were double those from fed rats, after 6-h incubation in the presence of 50 microM Zn, the fasted rat hepatocytes showed only half the MT concentrations of the fed rat hepatocytes. Glucagon and interleukin-6 (IL-6) were less effective inducers and increased MT synthesis by 28 and 17%, respectively. IL-6 (100 U/mL) was found to have an additive effect on MT synthesis above that of Zn alone (1-50 microM) or Zn plus dexamethasone (1 microM). A supernatant from LPS-stimulated macrophages increased MT synthesis by 40%. The basal MT synthesis was not increased by either tumor necrosis factor-alpha (TNF-alpha) or interleukin-1 (IL-1). All incubations were carried out in the presence of RPMI 1640 medium with Hepes (20 mM), bicarbonate (24 mM), and fatty acid-free albumin (FAFA; 0.5% w/v). MT synthesis was also seen using Krebs bicarbonate buffer with glucose (10 mM), Hepes (20 mM), and FAFA (0.5% w/v), and although the level of MT synthesis was less than in RPMI, the increases in concentrations of MT at 5 h were 225, 139, 36 and 20% for Zn, dexamethasone, glucagon, and control, respectively. It is concluded that MT synthesis occurs in freshly prepared hepatocytes and that these cells are responsive to some of the established inducers of MT. This system enables the study of MT synthesis in individual rats in various metabolic and pathological states.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Coyle P,Philcox JC,Rofe AMdoi
10.1007/BF02783778subject
Has Abstractpub_date
1993-01-01 00:00:00pages
35-49issue
1eissn
0163-4984issn
1559-0720journal_volume
36pub_type
杂志文章abstract::Atomic absorption spectrometry was used in a quantitative study of zinc, magnesium, and manganese on 71 postmortal human aortas. Samples were obtained from accident victims 35-65 years of age. Fibrous plaques had higher levels of all three elements when compared to normal aortic tissue. The difference was significant ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02916613
更新日期:1989-12-01 00:00:00
abstract::The study aimed at determination of the mineral composition of slimming herbal teas and estimation of the coverage of their total intake with infusions in women's daily diet. The content of Na+, K+, Ca+2, Mg+2, Zn+2, Cu+2, Fe+2, and Mn+2 was determined in infusions and mineralisates obtained from the slimming herbal t...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-016-0790-4
更新日期:2017-02-01 00:00:00
abstract::Iron-deficiency anemia (IDA) is the most common nutritional deficiency in childhood throughout the world. Although it has been shown that IRA is associated with elevated plasma copper and depleted zinc levels in children, there are conflicting results on the effect of iron supplementation on the absorption of these el...
journal_title:Biological trace element research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1385/BTER:94:1:79
更新日期:2003-07-01 00:00:00
abstract::The relationship between Ni-induced hepatic lipid peroxidation (LPO) and the concentrations of Ni and trace elements was investigated in male ICR mice. The protective effects of antioxidants were also examined. Hepatic LPO and the concentrations of Ni, Fe, Cu, and Zn in the liver were enhanced after an ip injection of...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784030
更新日期:1998-02-01 00:00:00
abstract::The effects of rare earth elements (REEs) not only on cell growth and flavonoid accumulation of Tetrastigma hemsleyanum suspension cells but also on the isoenzyme patterns and activities of related enzymes were studied in this paper. There were no significant differences in enhancement of flavonoid accumulation in T. ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9600-4
更新日期:2013-04-01 00:00:00
abstract::Retraction to: Biol Trace Elem Res 144(1–3):1120–1132, DOI 10.1007/s12011-011-9149-z. Article has been retracted due to duplicate publication. ...
journal_title:Biological trace element research
pub_type: 撤回出版物
doi:10.1007/s12011-012-9550-2
更新日期:2013-01-01 00:00:00
abstract::Relative stability of mineral elements in tissues is necessary for health. High static magnetic fields (HiSMFs) have been widely used in biomedical research and industry. However, the bioeffect of HiSMFs on animals is still unclear. In this study, we investigated the effects of HiSMF exposure on the levels of Mg, Fe, ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02469-1
更新日期:2021-01-07 00:00:00
abstract::The objective of this study was to investigate the effects of dietary supplemental chromium (Cr) on growth performance, carcass traits, and meat quality of broilers reared under heat stress. A total of 252 1-d-old Cobb 500 commercial female broilers were randomly allotted by body weight (BW) to one of six replicate ca...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-015-0443-z
更新日期:2016-03-01 00:00:00
abstract::Deficiency in the intake of trace elements, such as copper (Cu), iron (Fe), selenium (Se), and zinc (Zn), is very common in the general population of most developing countries. A preliminary study in India and Pakistan showing the plasma levels of Zn and Fe indicates that approx 50% of the subjects who participated ha...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02789092
更新日期:1998-03-01 00:00:00
abstract::Although fish consumption has positive health effects, metals accumulated in fish can cause human health risks. In this study, the levels of ten metals in rainbow trout (Oncorhynchus mykiss) farmed in the Keban Dam Reservoir, which has the biggest rainbow trout production capacity in Turkey, were determined and compar...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-017-1156-2
更新日期:2018-07-01 00:00:00
abstract::Male Sprague-Dawley rats, weighing 175-200 g, six per group were fed AIN 93M diet (CON) or diets containing 500 mg Ca (LCa), 7 mg Zn (LZn), 2 mg Cu (LCu), 60 mg Zn (HZn), or 12 mg Cu (HCu) per kilogram of diet in the following combinations: control (CON), LCa+LZn (LC+Z), LCa+LZn+LCu (LC+Z+C), or HZn+HCu (HZ+C) without...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:108:1-3:169
更新日期:2005-01-01 00:00:00
abstract::The metabolic significance of Se in plants is not well documented, though the presence of many selenoenzymes in bacteria and the essentiality of Se in higher animals is established. Since germination is an active process in plant growth and metabolism, the effect of Se was investigated in germinating Vigna radiata L, ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02789188
更新日期:1995-05-01 00:00:00
abstract::After considering the need for quality control in NAA, the concept of quality in NAA procedures themselves is discussed, and some important factors identified. Two approaches to improve quality are then described in more detail. The first concerns the unique ability of NAA using different isotopic reactions and differ...
journal_title:Biological trace element research
pub_type: 杂志文章,评审
doi:10.1007/BF02917356
更新日期:1994-10-01 00:00:00
abstract::This study deals with synthesis of chromium nanoparticles (CrNPs) from potassium dichromate using the aqueous extract of Allium sativum. They were characterized through UV-VIS light, FE-SEM, EDX, XRD, and FT-IR, which revealed uniform, mono-dispersive, and highly stable CrNPs of 31-64-nm size. The Artemia nauplii was ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1407-x
更新日期:2019-02-01 00:00:00
abstract::This study investigated the effects of ethanol (EtOH) on manganese (Mn)-induced striatal toxicity in rat by evaluating the neurobehavioral changes, biochemical and molecular events in rats exposed to Mn alone at 30 mg/kg, or their combination with EtOH at 1.25- and 5-g/kg body weight for 35 consecutive days. Locomotiv...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1587-4
更新日期:2019-09-01 00:00:00
abstract::Assessing the ability of a selenium (Se) sample to induce cellular glutathione peroxidase (GPx) activity in Se-deficient animals is the most commonly used method to determine Se bioavailability. Our goal is to establish a Se-deficient cell culture model with differential incorporation of Se chemical forms into GPx, wh...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-008-8097-8
更新日期:2008-07-01 00:00:00
abstract::A microcomputer-controlled irradiation and measurement system and a microprocessor-controlled sample changer have been installed at the SLOWPOKE-2 Facility at the Royal Military College of Canada (RMC). These systems can provide the gamut of instrumental neutron activation analysis (INAA) techniques for the analyst. C...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02992658
更新日期:1990-07-01 00:00:00
abstract::Heavy metals toxicity is a prevalent health problem particularly in developing countries. Mercury and cadmium are toxic elements that have no physiologic functions in human body. They should not be present in the human body by any concentration. Copper, on the other hand, is one of the elements that are essential for ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-015-0525-y
更新日期:2016-06-01 00:00:00
abstract::Interaction between toxic and essential elements is of particular interest, because the deficiency of essential element can dramatically increase the absorption rate of toxic metals inside the body. This study was conducted to evaluate the possible correlation of lead (Pb) with calcium (Ca), iron (Fe), and zinc (Zn) i...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-017-1141-9
更新日期:2018-06-01 00:00:00
abstract::This study was conducted to investigate the immune adherence function of erythrocytes and erythrocyte induced by dietary nickel chloride (NiCl2) in broilers fed on a control diet and three experimental diets supplemented with 300, 600, and 900 mg/kg NiCl2 for 42 days. Blood samples were collected from five broilers in...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-0096-3
更新日期:2014-11-01 00:00:00
abstract::The aim of this study was to evaluate the impact of three different chromium forms as chromic chloride (CrCl), chromium picolinate (CrPic), and a newly synthesized complex of chromium chelated with small peptides (CrSP) on glucose uptake and metabolism in vitro. In cultured skeletal muscle cells, chromium augmented in...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-009-8357-2
更新日期:2009-11-01 00:00:00
abstract::A dietary requirement is defined as the lowest continuing intake of a nutrient that for a specified indicator of adequacy, will maintain a defined level of nutriture in an individual. An essential dietary component is one that the body cannot synthesize in sufficient quantities to maintain health. Recommended dietary ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783138
更新日期:1998-01-01 00:00:00
abstract::Selective actinide ion recovery from dilute, aqueous, multication waste streams is an important problem. The recovery of uranium (U) and thorium (Th) by various animal biopolymers was examined. Of four species of biopolymers tested, a high uptake of uranium and thorium was found in hen eggshell membrane (ESM) and silk...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/bter:86:3:227
更新日期:2002-06-01 00:00:00
abstract::To examine whether there were differences between races in regard to age-related changes of elements and the relationships among elements in the arteries, the authors investigated both the renal arteries of Thai and Japanese. The Thai subjects consisted of 27 men and 11 women, ranging in age from 27 to 88 yr, whereas ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:106:3:219
更新日期:2005-09-01 00:00:00
abstract::The liver of Hooded Seal (Cystophora cristata) consists of six lobes of unequal sizes. Extensive sampling by means of cutout samples covering the depth horizon 2-30 mm below liver surface indicates that the liver may be considered homogeneous as to concentrations of zinc, cadmium, mercury, and selenium (residuals of o...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02789141
更新日期:1990-01-01 00:00:00
abstract::Three hundred and sixty healthy Ross × Ross 1-day-old broilers were used to study the effects of zinc glycine chelate (Zn-Gly) on oxidative stress, contents of trace elements, and intestinal morphology. All broilers were randomly assigned to six treatment groups, which replicates three times. Diets were as follows: (1...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-010-8824-9
更新日期:2011-09-01 00:00:00
abstract::Studies of effects of diazepam on liver parenchyma are very scanty. In this study, adult albino rats were treated with diazepam in two different doses (0.25 mg and 0.30 mg/kg body wt) daily for 30 and 60 d. Through light microscopy and electron microscopy, prenecrotic and necrotic changes were noted in the high-dose g...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02785292
更新日期:1997-06-01 00:00:00
abstract::Copper (Cu) parenteral administration is used in a beef cow-calf operations to prevent or correct Cu deficiency in bovines. At present, Zinc (Zn) salts have been incorporated to complement Cu antioxidant effect. A risk of hepatotoxicity generated by overdose is a negative consequence of injectable Cu application. Cu-Z...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-017-0965-7
更新日期:2017-10-01 00:00:00
abstract::Guizhou black goats are essential to the production system in the Wumeng prairie in the Western China. This study aimed to determine the influence of nano-copper on antioxidant system in copper-deprived Guizhou black goats. We analyzed mineral contents in soil, forage, and goats' tissues. Blood parameters were also de...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02342-1
更新日期:2020-08-18 00:00:00
abstract::In this study, the amount of reduced glutathione (GSH), oxidized glutathione (GSSG), and malondialdehyde (MDA) were determined by high performance liquid chromatography (HPLC), and selenium was determined by using the fluorescence spectrophotometer in eight different species of edible mushrooms. Brittlegill mushroom (...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-016-0715-2
更新日期:2016-12-01 00:00:00