Abstract:
:A gene assigned to human chromosome 1q32-41 encodes a novel protein of 3,113 amino acids containing an internal tandem repeat of 177 amino acids. The protein, which we have named "mitosin," was identified by direct binding to purified retinoblastoma protein in vitro with a region distantly related to the retinoblastoma protein-binding site of E2F-1. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G1. Its localization is dramatically reorganized from a rather homogeneous nuclear distribution in S phase to paired dots at the kinetochore/centromere region, to the spindle apparatus, and then to the midbody during M-phase progression. This spatial reorganization coincides closely with the temporal phosphorylation patterns of mitosin. Overexpression of N-terminally truncated mutants blocks cell cycle progression mainly at G2/M. These results suggest that mitosin may play an important role in mitotic-phase progression.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Zhu X,Mancini MA,Chang KH,Liu CY,Chen CF,Shan B,Jones D,Yang-Feng TL,Lee WHdoi
10.1128/mcb.15.9.5017subject
Has Abstractpub_date
1995-09-01 00:00:00pages
5017-29issue
9eissn
0270-7306issn
1098-5549journal_volume
15pub_type
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