Excitation mapping with the organic cation AGB2+.

Abstract:

:Excitation mapping is a method of visualizing the signaling history of neurons with permeant organic cations. It is compatible with high-resolution imaging, allowing concurrent visualization of all neuronal classes and their glutamate-gated excitation histories. Excitation mapping documents the stability and precision of neuronal signaling within a given neuronal class, arguing that single unit electrophysiological sampling accurately reflects neuronal diversity. We here review the theory of excitation mapping, provide methods and protocol links; outline imaging concepts; provide parametric data on the temporal range and physiological sensitivity of excitation mapping; and show that immunocytochemical methods for macromolecules are compatible with excitation mapping.

journal_name

Vision Res

journal_title

Vision research

authors

Marc RE,Kalloniatis M,Jones BW

doi

10.1016/j.visres.2005.07.025

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

3454-68

issue

28

eissn

0042-6989

issn

1878-5646

pii

S0042-6989(05)00386-X

journal_volume

45

pub_type

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