Abstract：
:The effects of oxytocin (OT) on holding current were studied in uterine smooth muscle cells freshly isolated from the longitudinal layer of 18-20 day pregnant rats, using the nystatin method of whole-cell voltage clamp. As we previously reported, the voltage-dependent Ca2+ current (L type) was partially inhibited by OT (about 30% inhibition at 1 microM). When the cells were held at the holding potential (HP) of -60 mV and the holding current was monitored, OT induced an inward current. The amplitude of this OT-induced current was 72 +/- 26 pA (n = 27). When the cell was held at more positive potentials (HP 0 or +40 mV), the OT-induced current reversed direction, becoming outward. This current usually was long lasting (74% of cells responding to OT); a transient current was observed in 26% of the cells. In the absence of either Na+ or Ca2+ in the bath solution, OT induced an inward current (at HP -60 mV). However, the OT-induced current was absent when both of these ions were omitted from the bath. These results suggest that OT induces an inward current through receptor-activated nonselective cation channels. The resulting increase of intracellular Ca2+ may contribute to the inhibition of voltage-dependent Ca2+ current produced by OT. This OT-induced current may also play an important role for membrane depolarization and accompanying contraction produced by OT in pregnant rat myometrium.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Shimamura K,Kusaka M,Sperelakis Ndoi
10.1139/y94-108subject
Has Abstractpub_date
1994-07-01 00:00:00pages
759-63issue
7eissn
0008-4212issn
1205-7541journal_volume
72pub_type
杂志文章abstract：:We previously reported that calpain, the Ca2+-sensitive cysteine protease, gets involved in atherogenesis. This study aimed to investigate the effects of calpain inhibitor I (CAI, 5 mg/kg per day) with or without NG-nitro-l-arginine-methyl ester (l-NAME) (100 mg/kg per day), the inhibitor of nitric oxide synthase (NOS...
journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/cjpp-2016-0652
更新日期：2018-01-01 00:00:00
abstract：:The main factors of myocardial ischemia are hypoxia, substrate deprivation, acidosis, and high extracellular potassium concentration ([K+]e), but the influence of each of these factors has not yet been evaluated in a cardiomyocyte (CM) culture system. Electromechanical responses to the individual and combined componen...
journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y02-143
更新日期：2002-12-01 00:00:00
abstract：:We used thermoluminescence, electron spin resonance, and viscosimetric measurements to establish whether or not a spice had been irradiated. Thermoluminescence, using the 1788 EN official protocol with an alternative method for the extraction of mineral impurities, led to proof of irradiation or proof of no treatment....
journal_title：Canadian journal of physiology and pharmacology
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更新日期：2001-02-01 00:00:00
abstract：:Longitudinal muscle of myometrial tissues from immature rats were examined by quantitative thin section electron microscopy for the presence of gap junctions after treatment with estradiol with and without tamoxifen, and cycloheximide for 1-6 days. Gap junctions were present between myometrial cells on days 4, 5, and ...
journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
abstract：:Hypertrophic scar (HS) results from abnormal wound healing, accompanied by excessive hypercellularity, migration and extracellular matrix (ECM) deposition. Autophagy dysregulation plays crucial roles during HS formation. The overexpressed p75 neurotrophin receptor (p75NTR) in injured skin tissue after wound healing be...
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journal_title：Canadian journal of physiology and pharmacology
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更新日期：1992-11-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
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更新日期：1993-12-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y77-025
更新日期：1977-04-01 00:00:00
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更新日期：2002-10-01 00:00:00
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更新日期：2015-08-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y81-103
更新日期：1981-07-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y86-194
更新日期：1986-08-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y87-361
更新日期：1987-11-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
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doi：10.1139/y2012-063
更新日期：2012-08-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
doi：
更新日期：1996-04-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
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更新日期：2012-05-01 00:00:00
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pub_type： 临床试验,杂志文章
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更新日期：1999-07-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
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doi：10.1139/y75-020
更新日期：1975-02-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
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更新日期：2009-02-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
doi：10.1139/y91-090
更新日期：1991-05-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y85-255
更新日期：1985-12-01 00:00:00
abstract：:Recent evidence suggests that some Ca2+ channels are regulated by a tyrosine kinase pathway. The possibility that Ca2+ entry is regulated by tyrosine kinase was investigated in canine vascular muscles. Functional studies were carried out in the dog mesenteric arteries and saphenous veins. Nicardipine-insensitive refil...
journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:The binding of 125I-labelled human chorionic gonadotropin (HCG) was studied using thick slices (300 micron) of rabbit ovarian tissue. Binding was saturable, reversible, stereospecific, and of high affinity. The amount of binding was proportional to the number of slices used and could be destroyed by boiling. Ovarian s...
journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y88-058
更新日期：1988-04-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y88-059
更新日期：1988-04-01 00:00:00
abstract：:Itracellular injections of Mg into cat spinal motoneurones have a depolarizing action, associated with a fall in input conductance, and depression of the postspike hyperpolarizing after-potential (a.h.p.) as well as its underlying conductance increase. There is also an increase in excitability, sometimes leading to ou...
journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：10.1139/y79-145
更新日期：1979-09-01 00:00:00
abstract：:This review presents, from the author's viewpoint, avenues of histamine research likely to produce new information. One potentially useful approach may be to attempt to relate histamine's function to its occurrence in different body tissues such as the pituitary and hypothalamus, the gastrointestinal mucosa, and the b...
journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
