Abstract:
:Hypertrophic scar (HS) results from abnormal wound healing, accompanied by excessive hypercellularity, migration and extracellular matrix (ECM) deposition. Autophagy dysregulation plays crucial roles during HS formation. The overexpressed p75 neurotrophin receptor (p75NTR) in injured skin tissue after wound healing becomes a factor aggravating scar. The study was designed to investigate the role of p75NTR and p75NTR-mediated autophagy in the process of HS. The results revealed that p75NTR expression was significantly upregulated while that of autophagy proteins was downregulated in cicatrix at 3 and 6 months after burn, which was recovered at 12 months. p75NTR silencing inhibited proliferation, migration and ECM deposition of hypertrophic scar fibroblasts (HSF), whereas p75NTR overexpression presented the opposite results. Silencing of p75NTR reduced the expression of PI3K/Akt/mTOR signaling molecules while enhanced that of autophagy proteins. Importantly, PI3K agonist (IGF-1) intervention notably decreased the levels of LC3B II/I and Beclin-1, and restored the inhibitory effects of p75NTR silencing on proliferation, migration and ECM deposition of HSF. Concurrently, autophagy inhibitor 3-methyladenine (3-MA) treatment exhibited the same variation trends with IGF-1. Taken together, these findings demonstrated that p75NTR silencing inhibits proliferation, migration and ECM deposition of HSF by activating autophagy through inhibiting PI3K/Akt/mTOR pathway.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Shi W,Wu Y,Bian Ddoi
10.1139/cjpp-2020-0219subject
Has Abstractpub_date
2020-07-29 00:00:00eissn
0008-4212issn
1205-7541pub_type
杂志文章abstract::The precise neurobiological mechanisms of tramadol abuse underlying the cognitive function are still unknown. The aim of the present study was to examine the possible effects of intra-CA1 injections of N-methyl-d-aspartate (NMDA), a glutamate NMDA receptor (NMDAR) agonist, and d,l-2-amino-5-phosphonopentanoic acid (DL...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2017-0228
更新日期:2018-01-01 00:00:00
abstract::Administration of d-fenfluramine, a serotonin-releasing drug, to male rats induced a dose-dependent increase in both serum prolactin and corticosterone concentrations. Serum growth hormone levels increased, but not significantly, at a dose of 1.25 mg/kg i.p. and decreased significantly at higher doses. When rats were ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y87-388
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abstract::The cause of embryonic thyroidal enlargement in the progeny of chickens fed goitrogenic rapeseed meal (RSM) has been investigated. It has been found that eggs from birds fed RSM are low in iodine. The low concentration of iodine in the eggs of the goitrous birds is apparently due to diversion of a large proportion of ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y76-038
更新日期:1976-06-01 00:00:00
abstract::Both gonadotropin-releasing hormone (GnRH) and prostaglandin F2 alpha (PGF2 alpha) can inhibit cAMP and progesterone production in the corpus luteum; however, their mechanism of action is not known. GnRH or PGF2 alpha causes a rapid and marked increase of labelling of phosphatidylinositol (PI) and phosphatidic acid (P...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/y85-046
更新日期:1985-03-01 00:00:00
abstract::We examined the interaction of smooth muscle myosin with alpha-actin and beta-actin isoforms during the contraction of A7r5 smooth muscle cells and rat aortic smooth muscle. The techniques of confocal microscopy and fluorescence resonance energy transfer (FRET) analysis were utilized in examining A7r5 cells and rat ao...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y09-008
更新日期:2009-05-01 00:00:00
abstract::Lingonberry grown in northern Manitoba, Canada, contains exceptionally high levels of anthocyanins and other polyphenols. Previous studies from our lab have shown that lingonberry anthocyanins can protect H9c2 cells from ischemia-reperfusion injury and anthocyanin-rich diets have been shown to be associated with decre...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2016-0667
更新日期:2017-08-01 00:00:00
abstract::In the present study we characterized the receptor(s) that mediates non-adrenergic non-cholinergic (NANC) contractions of isolated guinea pig cervical trachea, using CP-99,994, a selective neurokinin (NK1) receptor antagonist, and SR-48,968, a selective neurokinin (NK2) receptor antagonist. The activity of these two a...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y94-028
更新日期:1994-02-01 00:00:00
abstract::Although amiodarone is a highly efficacious antidysrhythmic agent, the drug produces numerous adverse effects. The most critical of these is pulmonary toxicity because of the potential for mortality. This review examines the experimental model systems used to study amiodarone toxicity, summarizes the current state of ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/y95-730
更新日期:1995-12-01 00:00:00
abstract::In Western societies, the incidence of diet-related diseases is progressively increasing due to greater availability of hypercaloric food and a sedentary lifestyle. Obesity, diabetes, atherosclerosis, and neurodegeneration are major diet-related pathologies that share a common pathogenic denominator of low-grade infla...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/cjpp-2012-0307
更新日期:2013-06-01 00:00:00
abstract::The release of endothelium-derived relaxing factor (EDRF), prostacyclin (PGI2), and endothelin-1 (ET-1) was measured from endothelial cells (EC) cultured from either bovine vena cava (BVCEC) or bovine aorta (BAEC). EDRF release was determined by using the superfusion bioassay technique, whereas ET-1 and PGI2 were meas...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y92-088
更新日期:1992-05-01 00:00:00
abstract::The natural history of the disease of the dilated strain Bio TO-2 of cardiomyopathic hamsters (CMH) is not totally characterized. We investigated its hemodynamic and histomorphometric characteristics at 140, 180, 220, 260, and 300 days of age. Forty CMH and 40 controls were investigated (8 at each stage). Mean arteria...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::The response pattern of the isolated longitudinal muscle of the rabbit ileum to adenosine-5'-alpha, beta-methylenediphosphonate (APCP) was found to differ from that caused by adenosine or ATP in that the rapid initial phase of relaxation is absent and that at doses above 10 muM a secondary increase of contractile acti...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y76-087
更新日期:1976-08-01 00:00:00
abstract::To elucidate the mechanism of enhanced insulin sensitivity by muscle after exercise, we studied insulin binding, 2-deoxy-D-[1-14C]glucose (2-DOG) uptake and [5-3H]glucose utilization in glycolysis and glycogenesis in soleus and extensor digitorum longus (EDL) muscles of mice after 60 min of treadmill exercise. In the ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y84-248
更新日期:1984-12-01 00:00:00
abstract::The ability of dystrophic hamsters to maintain their body temperature despite abnormal muscle and brown adipose tissue, two organs involved in thermoregulation, was evaluated. Dystrophic hamsters (CHF 146) between the ages of 30 and 160 days kept at 21 degrees C had core (rectal) temperatures (TR) that were 0.5-1.5 de...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y85-187
更新日期:1985-09-01 00:00:00
abstract::Microglial inflammation, involved in the occurrence and development of sepsis-associated encephalopathy, exhibits upregulation of proinflammatory cytokine and proinflammatory enzyme expression, leading to inflammation-induced neuronal cell apoptosis. TIR domain containing adaptor molecule-2 (TICAM-2) participates in l...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2019-0699
更新日期:2020-11-01 00:00:00
abstract::Because the excessive apoptosis of articular chondrocytes contributes to extracellular matrix (ECM) loss and cartilage damage in rheumatoid arthritis (RA), inhibiting chondrocyte apoptosis might be a promising strategy for RA. Aquaporin1 (AQP1) is overexpressed in RA cartilage and synovial tissues, and play a vital pa...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2018-0334
更新日期:2018-11-01 00:00:00
abstract::The effects of L-641,953 (R-8-fluoro-dibenzo[b, f]thiepin-3-carboxylic acid-5-oxide) have been studied on pulmonary and other smooth muscle preparations in vitro and in vivo. When studied in vitro on guinea-pig tracheal chains, L-641,933 produced significant shifts in the dose-response curves to the prostaglandin endo...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y87-087
更新日期:1987-04-01 00:00:00
abstract::In male Sprague--Dawley rats, hypertensive development was diminished for 10 weeks when parathyroidectomy (PTX) was performed 1 week before deoxycorticosterone plus saline (DOCA + NaCl) treatment. In young spontaneously hypertensive male rats (SHR, Okamoto strain) parathyroidectomy performed after weaning lessened hyp...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y82-033
更新日期:1982-02-01 00:00:00
abstract::The mechanism whereby chronic rapid ventricular pacing induces severe heart failure is unclear, but the phenomenon is associated with a reduction in left ventricular ATP levels. Accordingly, the current study was undertaken to evaluate the acute effects of rapid ventricular pacing on hemodynamics, left ventricular ade...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y94-002
更新日期:1994-01-01 00:00:00
abstract::A new antagonist of the vasoconstrictor eicosanoids, L-640,035, was studied in a standardized model of myocardial ischemia (MI) in anesthetized cats. This eicosanoid antagonist was not found to exert any overt hemodynamic action in cats subjected to a sham myocardial ischemia protocol. However, the antagonist markedly...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y84-246
更新日期:1984-12-01 00:00:00
abstract::The effects of beta-adrenergic blocking agents, timolol and atenolol (1-1000 microM), were studied on rat heart sarcolemmal ATPase and Ca2+ binding activities. Timolol, unlike atenolol, increased both Ca2+-stimulated ATPase and ATP-dependent Ca2+ binding; the maximal effects were seen at 1 microM concentration of timo...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y83-037
更新日期:1983-03-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y81-100
更新日期:1981-07-01 00:00:00
abstract::Angiotensin II (ANG II) causes a systemic pressor effect when injected into the cerebral ventricles. In the rat fourth ventricle, the effective doses for the ANG II pressor effect are over 100 times larger than in the systemic circulation. Considering the discrepancy of doses, the possibility that ANG II may reach the...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y02-062
更新日期:2002-05-01 00:00:00
abstract::Effects of xanthine--xanthine oxidase produced oxygen radicals were studied in hypertrophied rat hearts in a Langendorff preparation. Heart hypertrophy was produced by banding of the abdominal aorta for 6 weeks. This resulted in a 22% increase in ventricle/body weight ratio compared with that of sham-operated controls...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y87-183
更新日期:1987-06-01 00:00:00
abstract::A brief burst of electrical stimuli delivered to the vagus nerve during the cardiac cycle elicits a triphasic cardiac chronotropic response. The cardiac cycle length initially increases, then briefly decreases, and subsequently increases again. We studied the effects of a calcium channel blocking agent, verapamil, on ...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y86-164
更新日期:1986-07-01 00:00:00
abstract::A brief summary is given of some of the key points of the individual contributions at this International Brain Research Organization satellite meeting, presenting up-to-date, expert knowledge of energy metabolism, ionic turnover, and cell swelling at the cellular level of the mammalian central nervous system. On the b...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:10.1139/y92-250
更新日期:1992-01-01 00:00:00
abstract::In vivo fatty acid synthesis and the pathways of glycerol-3-phosphate (G3P) production were investigated in brown adipose tissue (BAT) from rats fed a cafeteria diet for 3 weeks. In spite of BAT activation, the diet promoted an increase in the carcass fatty acid content. Plasma insulin levels were markedly increased i...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y08-052
更新日期:2008-07-01 00:00:00
abstract::Chrysin (CH) is a natural flavonoid with pharmacological influences. The purpose of the current study was the assessment of possible protective effects of CH against oxidative damage in the serum, liver, brain, and pancreas of streptozotocin (STZ)- induced diabetic rats. In the present study, the rats were divided int...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2014-0412
更新日期:2016-04-01 00:00:00
abstract::The effect of estrogen on the passive characteristics of arteries is not known. We hypothesized that estrogen would increase arterial distensibility as part of its protective effect on the vasculature. Female Sprague-Dawley rats were ovariectomized at 11 weeks of age. One group received a placebo (n = 6), while two ot...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-77-1-75
更新日期:1999-01-01 00:00:00
abstract::We hypothesized that the respiratory baroreflex in conscious rats is either more transient, or has a higher pressure threshold than in other species. To characterize the effect of arterial pressure changes on respiration in conscious rats, ventilation (V) was measured by the plethysmographic technique during injection...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-76-7-8-707
更新日期:1998-07-01 00:00:00