[Frequency of K-ras mutation in biliary and pancreatic tumors].

Abstract:

BACKGROUND:The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas. AIM:To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors. MATERIAL AND METHODS:Samples of 35 gallbladder, 15 ampulla of Vater, 10 biliary tract and 9 pancreatic tumors, were analyzed. The tumor tissue was microdissected from paraffin embedded biopsies. The mutation was detected by a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). RESULTS:Overall, 46% of samples had K-ras gene mutations. Mutation frequency was 80, 56, 50 and 29% for ampulla of Vater, pancreatic, biliary tract and gallbladder tumors, respectively. When compared with the rest, gallbladder tumors had a significantly lower frequency of the mutation. Median survival for biliary tract tumors was 6 months, compared with 65 months for gallbladder tumors (p <0.05). CONCLUSIONS:Gallbladder carcinoma had the lower frequency of K-ras mutation, when compared with pancreatic, biliary tract and ampulla of Vater tumors.

journal_name

Rev Med Chil

journal_title

Revista medica de Chile

authors

Roa JC,Anabalón L,Tapia O,Melo A,de Aretxabala X,Roa I

doi

/S0034-98872005001200004

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

1434-40

issue

12

eissn

0034-9887

issn

0717-6163

pii

S0034-98872005001200004

journal_volume

133

pub_type

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