Fecal specimens preparation methods for PCR diagnosis of human taeniosis.

Abstract:

:Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit. Best DNA extraction results were achieved after eggs disruption with glass beads, either with phenol/chloroform/isoamilic alcohol, DNAzol reagent or QIAmp DNA stool mini kit.

authors

Nunes CM,Lima LG,Manoel CS,Pereira RN,Nakano MM,Garcia JF

doi

10.1590/s0036-46652006000100010

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

45-7

issue

1

eissn

0036-4665

issn

1678-9946

pii

S0036-46652006000100010

journal_volume

48

pub_type

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