Abstract:
:A comparative study of the N-B transition and the drug binding properties of human serum albumin samples from various sources has been carried out with the help of circular dichroism and equilibrium dialysis. It was found that when warfarin was used as a marker the midpoint pH and the cooperative nature of the N-B transition that occurs in the albumin around physiological pH varied with the albumin sample. The midpoint pH was found to be related to the cooperative nature of the albumin samples. A similar relationship has been found for allosteric proteins. However, when diazepam was used as a marker molecule for the N-B transition, variations in the midpoint pH and cooperative nature of the N-B transition disappeared. This is attributed to the strong allosteric effect of diazepam on binding. The affinity of warfarin for albumin depends strongly on the sample, but this is not the case with diazepam. The cooperative binding properties found for the albumin samples are compared with those found for the albumin in serum. After a discussion it is concluded that the cooperative binding properties of a particular albumin sample should be taken into account when that sample is used in binding studies.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Dröge JH,Wilting J,Janssen LHdoi
10.1016/0006-2952(82)90293-3subject
Has Abstractpub_date
1982-12-01 00:00:00pages
3781-6issue
23eissn
0006-2952issn
1873-2968pii
0006-2952(82)90293-3journal_volume
31pub_type
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