Abstract：
OBJECTIVE:Our purpose was to determine the mRNA and protein expression of cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in ovarian tumors and normal ovaries of the hen, which is an excellent model for human ovarian cancer. Tissue concentrations of prostaglandin E(2) (PGE2) and PGE2 metabolites were also determined. METHODS:Tissue was obtained from ovarian tumor (n = 18) and normal ovary (n = 29) of 2- to 4-year old Single-comb White Leghorn hens. Quantitative real-time PCR with Sybr Green was used to quantify the mRNA expression of COX-1 and COX-2, using 18S expression as an internal control for COX normalization. Immunohistochemistry using antibodies for COX-1 and COX-2 was used to localize protein expression of each isoform in a subset of tumor (n = 5) and normal samples (n = 6). For determination of tissue prostaglandin concentration, tissue was obtained from ovarian tumor (n = 8) and normal ovary (n = 8). PGE2 and PGE2 metabolites were measured using competitive enzyme immunoassays (EIAs). RESULTS:Our results indicate that COX-1 mRNA expression is significantly higher (P < 0.05) in ovarian tumor samples compared to normal ovaries while there is no significant difference in expression of COX-2 between the samples. Immunohistochemistry results support this finding and show COX-1 expression only in tumor samples and COX-2 expression unchanged between normal ovary and tumor samples. PGE2 levels are significantly higher (P < 0.05) in tumor samples compared to normal ovaries, and there is no significant difference in PGE2 metabolite levels between the samples. CONCLUSION:These findings may implicate COX-1 as a suitable target for the prevention or treatment of ovarian cancer.
journal_name
Gynecol Oncoljournal_title
Gynecologic oncologyauthors
Urick ME,Johnson PAdoi
10.1016/j.ygyno.2006.05.012subject
Has Abstractpub_date
2006-11-01 00:00:00pages
673-8issue
2eissn
0090-8258issn
1095-6859pii
S0090-8258(06)00378-7journal_volume
103pub_type
杂志文章abstract：OBJECTIVE:Small cell carcinoma (SmCC) of the female genital tract constitutes a diagnostic and clinical challenge given its rarity and the lack of standardized therapeutic approaches. Here we review the morphological, clinical and molecular features of gynecologic SmCCs and discuss potential areas for future research. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2014.05.017
更新日期：2014-08-01 00:00:00
abstract：:The ovary is a relatively frequent site of metastasis from malignant neoplasia arising elsewhere in the body, the majority of these originating from the GI tract. The best known tumor of this type is signet-ring cell adenocarcinoma (Krukenberg tumor) of gastric origin. The gall bladder and bile ducts are rare sources ...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/0090-8258(92)90120-8
更新日期：1992-11-01 00:00:00
abstract：:Human papillomavirus (HPV) containing cell lines derived from human cervical intraepithelial neoplasia (CIN) can offer valuable insights into the role of HPV in cervical neoplasia and can help in the understanding of the cellular changes that fuel the progression toward malignancy. We describe the growth and different...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1993.1131
更新日期：1993-06-01 00:00:00
abstract：OBJECTIVE:Self-collected HPV testing could substantially reduce disparities in cervical cancer screening, with slightly lower sensitivity compared to physician-collected specimens cross-sectionally. We aimed to evaluate the comprehensive long-term performance of self-collected HPV testing prospectively. METHODS:In 199...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.09.012
更新日期：2018-11-01 00:00:00
abstract：:Eighteen (18) previously untreated patients with grade I (9) and II (9) endometrial adenocarcinoma provided endometrial samples for biochemical analysis of estrogen and progesterone receptors. The same samples were evaluated by histology and ultrastructure. Progesterone receptor negative samples (PR less than 1000 fmo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(84)90022-2
更新日期：1984-06-01 00:00:00
abstract：:Thirty patients with uterine papillary serous carcinoma were treated with intravenous cisplatin, doxorubicin, and cyclophosphamide (CAP) chemotherapy, a combination with proven efficacy against ovarian carcinoma. Nineteen patients were given CAP as an adjuvant soon after surgery. Eleven patients were treated after rec...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1006/gyno.1993.1308
更新日期：1993-12-01 00:00:00
abstract：BACKGROUND:Advanced gynecological surgery undertaken in a specialized gynecologic oncology unit may be associated with significant perioperative morbidity. Validated risk prediction models are available for general surgical specialties but currently not for gynecological cancer surgery. OBJECTIVE:The objective of this...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2009.08.004
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:To investigate the role of Bcl-X(L) on the growth and survival of human ovarian carcinoma cells. METHODS:In this study, we enforced down-regulation of Bcl-X(L) by RNA interference in ovarian carcinoma cell lines CaOV3, SKOV3ip1 and OVCAR3 cell lines expressing various levels of Bcl-X(L). We also established ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.08.028
更新日期：2006-02-01 00:00:00
abstract：:This report presents 13 patients with epithelial ovarian neoplasms of low malignant potential. Ten patients had serous tumors, 1 had a mucinous tumor, and 2 had endometrioid tumors. Two had Stage I disease, 4 had Stage II disease, and 7 had Stage III disease. All were treated with melphalan. Second-look laparotomy was...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(85)90250-1
更新日期：1985-06-01 00:00:00
abstract：PURPOSE:To examine the differentially expressed cancer-related genes in two morphologically different uterine cervical carcinoma cell lines derived from the same patient by an Affymetrix Human Cancer G110 Array carrying 1700 cancer-associated genes. In addition, to investigate specific gene expression depending on hist...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.02.012
更新日期：2004-05-01 00:00:00
abstract：:The steroid receptor content of the primary endometrial cancer of 22 patients who were treated for recurrent or advanced disease has been measured and correlated with response to medroxyprogesterone acetate. No patient with a progesterone receptor (PR)-negative tumor responded and only 2 patients with PR-positive tumo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(85)90269-0
更新日期：1985-07-01 00:00:00
abstract：:HIV infection among women is increasing in the United States. Since January 1993, cervical cancer has been considered an AIDS-defining illness. We report a rare case of squamous cell carcinoma arising in a teratoma of the uterine cervix in an HIV-infected patient. This patient was treated with a radical hysterectomy, ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1996.0076
更新日期：1996-03-01 00:00:00
abstract：OBJECTIVE:This two-arm longitudinal study was performed within a regional organized cervical-cancer-prevention program in which HPV-DNA test is used in primary screening. The aim was to analyze the diagnostic performances of p16INK4a/Ki-67 dual-test and E6/E7-mRNA test in identifying CIN2+ lesion among HPV-DNA positive...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.11.030
更新日期：2013-03-01 00:00:00
abstract：:The finding of osseous metaplasia within an ovarian papillary serous cystadenocarcinoma is very rare. A review of the medical literature reveals only two previously published cases where mature bone was found in an ovarian serous cystadenocarcinoma. This report presents an additional case of this unusual phenomenon wh...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(90)90439-r
更新日期：1990-11-01 00:00:00
abstract：:The usefulness of percutaneous endoscopic gastrostomy (PEG) for decompression in patients with unresolving intestinal obstruction by gynecological malignancy is examined. Between April 1993 and August 1995, 34 consecutive patients with small-bowel obstruction by gynecological cancer, heavily pretreated with surgery an...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1006/gyno.1996.0197
更新日期：1996-07-01 00:00:00
abstract：OBJECTIVE:Different Human Papilloma Virus (HPV) tests are currently used. An integrated comparison of the Amplicor, Cobas4800, PreTect HPV-Proofer and APTIMA HPV tests has not been done. METHODS:We compared the high-risk HPV detection power of these HPV tests in 528 consecutive population-based follow-up Liquid-Based ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2011.07.024
更新日期：2011-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the impact of introducing a robotics program on clinical outcome of elderly patients with endometrial cancer. METHODS:Evaluation and comparison of peri-operative morbidity and disease-free interval in 163 consecutive elderly patients (≥70years) with endometrial cancer undergoing staging procedure...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2014.03.572
更新日期：2014-06-01 00:00:00
abstract：:Classification of ovarian tumors according to FIGO does not take into account involvement of spleen. A patient who appears to be the first reported case of widespread carcinoma of the ovary with involvement of the splenic parenchyma is presented. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(88)90058-3
更新日期：1988-05-01 00:00:00
abstract：:Toxic shock syndrome is a multisystem disease which presents with a high fever, rash, gastrointestinal symptoms, and hypotension. A 58-year-old woman presented to the emergency room with these symptoms, hypotension and tachycardia. Because of vaginal spotting a pelvic examination was performed which demonstrated vulvo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1994.1043
更新日期：1994-02-01 00:00:00
abstract：OBJECTIVE:To determine if tumor-free distance (TFD) from the uterine serosa predicts surgicopathologic factors and outcome in surgically staged endometrial cancer, and to compare TFD with the traditional estimate of depth of myometrial invasion (DOI). METHODS:Patients who underwent complete surgical staging for primar...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2008.09.009
更新日期：2009-01-01 00:00:00
abstract：OBJECTIVE:To evaluate clinicopathological factors and survival outcome of patients with advanced epithelial ovarian carcinoma undergoing multiple bowel resections to achieve optimal (< or = 1 cm) cytoreduction. METHODS:A case-control study was performed identifying patients undergoing optimal primary cytoreductive sur...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2007.08.003
更新日期：2007-12-01 00:00:00
abstract：OBJECTIVE:To profile long non-coding RNA (lncRNA) expression at the various anatomic sites of high-grades serous carcinoma (HGSC) and in effusion-derived exosomes. METHODS:LncRNA profiling was performed on 60 HGSC specimens, including 10 ovarian tumors, 10 solid metastases and 10 malignant effusions, as well as exosom...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2018.01.004
更新日期：2018-03-01 00:00:00
abstract：:Stains for mucosubstances were performed on endometrial curetting material of normal and pathological endometrium. The study included normal proliferative and secretory endometrium, cystic and adenomatous hyperplasia, and endometrial adenocarcinoma. It was attempted to characterize the epithelial mucin in each of thes...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(88)90033-9
更新日期：1988-06-01 00:00:00
abstract：OBJECTIVE:Epithelial ovarian cancer continues to be the deadliest gynecologic malignancy. Patients with both diabetes mellitus and obesity have poorer outcomes, yet research correlating metabolic abnormalities, such as metabolic syndrome, to ovarian cancer risk and outcomes is lacking. This article reviews the literatu...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2016.10.005
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Glucocorticoid receptor (GR) has emerged as an important steroid nuclear receptor in hormone dependent cancers, however few data are available regarding a potential role of GR in endometrial cancer. The aim of this study was to investigate expression of GR in primary and metastatic endometrial cancer lesions...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2017.09.013
更新日期：2017-12-01 00:00:00
abstract：:Over a 20-year period, 34 patients with FIGO stage II ovarian carcinoma were treated with postoperative pelvic irradiation at the University of Michigan. Complications of radiation treatment were minimal. The overall actuarial disease-free 5-year survival was 53%. This was not significantly different for substages IIA...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(88)90142-4
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVES:We have previously shown that high activity of lactate dehydrogenase (LD) isoenzymes 4 and 5 in terine aspirates is a marker for endometrial carcinoma. The purpose of this study was to identify an abnormal activity profile of LD2-5 using LD1 as an internal standard, thereby being able to dispense with measur...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.02.014
更新日期：2004-05-01 00:00:00
abstract：:PI3K/AKT/mTOR pathway is a crucial mediator of tumor progression. As the PI3K/Akt pathway is heavily deregulated in breast cancer, the application of mTOR inhibitors in breast cancer patients seems warranted. This is the first systematic review according to PRISMA guidelines to synthesize all available data of mTOR in...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2012.08.040
更新日期：2012-12-01 00:00:00
abstract：:The Cavitron Ultrasonic Surgical Aspirator (CUSA) can be used to fragment and aspirate tissue precisely. The CUSA was utilized as an adjunctive procedure to standard operative techniques to optimally debulk 11 women with advanced epithelial carcinoma of the ovary. No significant intraoperative or postoperative complic...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(88)90298-3
更新日期：1988-09-01 00:00:00
abstract：:Tumor specimens from 92 patients with ovarian carcinoma were analyzed for estrogen receptor (ER), progesterone receptor (PR), proliferative fraction, and ploidy. Seventy-one percent of tumors were either ER+ (greater than 5 fmole/mg protein) or PR+ (greater than 10 fmole/mg protein) with 27% of tumors overall being bo...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/s0090-8258(89)80031-9
更新日期：1989-02-01 00:00:00
