Abstract：
:Insulin-like growth factor I (IGF-I) has been found in the kidney, particularly in the collecting duct in the rat. Since cultured rabbit collecting duct cells constitute a convenient system for in vitro studies, we have examined whether these cells secrete IGF-I. Culture medium conditioned by collecting duct cells was concentrated by reverse phase chromatography and applied to a Sephadex G100 column equilibrated in a denaturing buffer. Two major species with apparent molecular weights of 7.5 and greater than 25 kilodaltons (kD) were identified by IGF-I RIA. A smaller amount of 10 kD species was also observed. Further characterization of 7.5 kD IGF-I immunoreactive species by reverse phase HPLC showed that it eluted in a single peak. To determine whether the higher molecular weight species possessed IGF-I binding activity, appropriate fractions were desalted, incubated with [125I]IGF-I (thr59) for two hours at 30 degrees C and applied to a Sephadex G100 column equilibrated in a non-dissociating buffer. The major peak of radioactivity was confined to a high molecular weight region; there was no radioactivity in the fractions corresponding to 7.5 kD. Western ligand analysis of unreduced conditioned medium identified two IGF-I binding species of 25 and 30 kD, similar in size to species observed in normal rabbit serum. 125I-IGF-I binding as assessed in a charcoal adsorption assay could be displaced by IGF-I and IGF-II but not by insulin. Further characterization of the 10 kD peak of IGF-I immunoreactivity indicated that it did not possess IGF-I binding activity.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Aron DC,Saadi HF,Nye CN,Douglas JGdoi
10.1038/ki.1991.3subject
Has Abstractpub_date
1991-01-01 00:00:00pages
27-32issue
1eissn
0085-2538issn
1523-1755pii
S0085-2538(15)57111-1journal_volume
39pub_type
杂志文章abstract：:Changes in tissue oxygen levels trigger molecular signaling pathways that regulate cellular proliferation and differentiation in multiple cell types. The functional role of oxygen signaling in the immune system is not well understood. Rama and colleagues demonstrate that hypoxia induces dendritic-cell maturation; thus...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.15
更新日期：2008-04-01 00:00:00
abstract：:The effects of acidosis on renal function and morphology were examined in the isolated perfused rat kidney (IPK). Kidneys were perfused with oxygenated Krebs-Henseleit-albumin medium for 60 minutes at pH 7.4 or pH 7.0. At the lower pH, GFR was reduced by 25%, TRNa by 32% and oxygen consumption by 41% as compared to pe...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.251
更新日期：1988-12-01 00:00:00
abstract：BACKGROUND:Cux-1 is a murine homeobox gene that is highly expressed in the nephrogenic zone of the developing kidney. Transgenic mice ectopically expressing Cux-1 develop renal hyperplasia associated with down-regulation of the cyclin kinase inhibitor p27. Because the reduction of p27 has been associated with mesangial...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00889.x
更新日期：2003-04-01 00:00:00
abstract：:Congenital nephrotic syndrome is clinically and genetically heterogeneous. The majority of cases can be attributed to mutations in the genes NPHS1, NPHS2, and WT1. By homozygosity mapping in a consanguineous family with isolated congenital nephrotic syndrome, we identified a potential candidate region on chromosome 3p...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5001679
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND:Recent advances in the understanding of the molecular biology of rodent renal development have lead to the ability to culture the components of the developing rat kidney-the ureteric bud (UB) and the metanephric mesenchyme (MM)-in isolation from one another. Here we here describe a method for subculturing an...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00694.x
更新日期：2002-12-01 00:00:00
abstract：:The sympathetic nervous system modulates renal function through its receptors namely beta1 (cardiac output and renin release), alpha1 (systemic and renovascular constriction), and beta2 renovascular dilation. Sympathetic overactivity is commonly seen in chronic kidney disease (CKD) and is an important contributor to i...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5001835
更新日期：2006-12-01 00:00:00
abstract：:An effective therapeutic means to remove relatively large polypeptide uremic toxins seems to be a hemofiltration (HF) or hemodiafiltration (HDF) employing a larger-pore membrane, that is, a protein-permeable membrane. With either method, however, a significant amount of albumin will be lost into the ultrafiltrate or d...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.333
更新日期：1996-08-01 00:00:00
abstract：:Intradialytic hypotension is a most frequent complication of hemodialysis and may contribute to cardiovascular events and high mortality. There is a hypothesis that an increase in adenosine generation during hemodialysis may cause vasodilation and a decrease in cardiac output, which results in systemic hypotension. We...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/sj.ki.5000088
更新日期：2006-03-01 00:00:00
abstract：:The introduction of two-photon microscopy, along with the development of new fluorescent probes and innovative computer software, has advanced the study of intracellular and intercellular processes in the tissues of living organisms. Researchers can now determine the distribution, behavior, and interactions of labeled...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5002315
更新日期：2007-08-01 00:00:00
abstract：:The binding of albumin to the glomerular capillary wall was studied using albumin-gold in perfused kidneys, the interaction of [3H]albumin with isolated glomeruli at 37 degrees C and 4 degrees C and the interaction at [3H]albumin with purified basement membrane. The albumin-gold was found to bind predominantly to the ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.149
更新日期：1995-04-01 00:00:00
abstract：:Residual kidney function (RKF) may confer a variety of benefits to patients on maintenance dialysis. RKF provides continuous clearance of middle molecules and protein-bound solutes. Whereas the definition of RKF varies across studies, interdialytic urine volume may emerge as a pragmatic alternative to more cumbersome ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1016/j.kint.2016.02.037
更新日期：2016-08-01 00:00:00
abstract：:The acutely injured mammalian kidney mounts a cellular and molecular response to repair itself. However, in patchy regions such intrinsic processes are impaired and dysregulated leading to chronic kidney disease. Currently, no therapy exists to treat established acute kidney injury per se. Strategies to augment human ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1016/j.kint.2017.07.030
更新日期：2018-01-01 00:00:00
abstract：:The rate of myofibrillar protein degradation was measured by 3-methylhistidine (3MH) release in moderately uremic and sham-operated control rats. The rats were studied in the fed state or after 24 and 48 hours of fasting. When fed, both uremic and control rats gained weight at the same rate. During 48 hours of fasting...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1981.141
更新日期：1981-09-01 00:00:00
abstract：BACKGROUND:Although atherosclerotic renovascular disease is increasingly recognized in chronic kidney disease, few national level studies have examined its clinical epidemiology. METHODS:Claims data from a 5% random sample of the United States Medicare population were used to select patients without atherosclerotic re...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00406.x
更新日期：2005-07-01 00:00:00
abstract：:Suzuki et al. analyzed a mouse model of erythropoietin-deficient anemia to show that during iron overload renal interstitial fibroblasts accumulate iron, and this impairs the hypoxia-driven transcription of the erythropoietin gene. The authors show that excess iron decreases levels of hypoxia-inducible transcription f...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2018.07.027
更新日期：2018-11-01 00:00:00
abstract：:The pathogenesis of the multiple structural lesions in diabetic nephropathy remains debated, and likely is multifactorial. The uniform thickening of the renal basement membranes lining the glomerular and tubular elements appears to be a consequence of the metabolic perturbations which are directly related to hyperglyc...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1993.19
更新日期：1993-01-01 00:00:00
abstract：:Recent studies have greatly expanded our knowledge of initial events that lead to epithelial cell polarity. Epithelial polarity is defined, in part, by apical cell-cell tight junctions that separate the plasma membrane into the apical domain and the basolateral domain, as well as the zonula adherens that mediate inter...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5002579
更新日期：2007-12-01 00:00:00
abstract：BACKGROUND:Interleukin-6 (IL-6) exerts its actions through a cell-surface receptor system that consists of two transmembrane subunits: the IL-6 binding glycoprotein gp 80 (IL-6R) and the signal-transducing component (gp 130). Soluble forms of the IL-6R (sIL-6R) are generated by shedding of the membrane-associated prote...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00181.x
更新日期：2000-07-01 00:00:00
abstract：:Proteomics-based assays have substantially improved diagnostic accuracy in systemic amyloidosis and have led to the discovery of novel amyloidogenic proteins. The observation that human apolipoproteins are increasingly represented is intriguing and highlights the complex, and partially known, mutual effects of the mis...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.05.029
更新日期：2016-09-01 00:00:00
abstract：:Monocyte chemoattractant protein 1 (MCP-1) is a C-C chemokine with potent monocyte chemotactic and activating properties that may contribute to glomerular macrophage infiltration in anti-GBM Ab GN. We have previously reported increased glomerular steady state expression of MCP-1 mRNA relative to GAPDH mRNA in the hete...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.363
更新日期：1996-08-01 00:00:00
abstract：:The transition between the glomerulus and the proximal tubule was studied by light and electron microscopy in perfusion-fixed rabbit kidneys. In most nephrons a transitional segment of tubule occurred between the Bowman's capsule and the proximal tubule proper. This initial part of the tubule was lined by flattened ce...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1975.22
更新日期：1975-03-01 00:00:00
abstract：:As the worldwide prevalence of end-stage renal disease increases it is important to evaluate the rate of living kidney donation in various countries; however there is no comprehensive global assessment of these rates. To measure this, we compiled data from representatives, renal registries, transplant networks, publis...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.20
更新日期：2009-05-01 00:00:00
abstract：:Defective cholesterol metabolism primarily linked to reduced ATP-binding cassette transporter A1 (ABCA1) expression is closely associated with the pathogenesis and progression of kidney diseases, including diabetic kidney disease and Alport Syndrome. However, whether the accumulation of free or esterified cholesterol ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2020.06.040
更新日期：2020-11-01 00:00:00
abstract：:NO, a simple molecule synthesized from L-arginine by NO synthases, has been identified to play an important role in cell communication, cell defense and cell injury. The half life of NO is very short because NO either reacts with superoxide anion (O2-), and/or binds to heme molecules or Fe-S groups present in proteins...
journal_title：Kidney international
pub_type： 社论,评审
doi：10.1038/ki.1995.262
更新日期：1995-07-01 00:00:00
abstract：:Platelet-derived growth factor (PDGF)-D, a specific PDGF receptor β (PDGFR-β) ligand, mediates mesangial proliferation in vitro and in vivo. However, its role in renal development, physiology, and fibrosis is relatively unknown. In healthy murine kidneys, PDGF-D was found to be expressed on renal mesenchymal cells (me...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2015.12.037
更新日期：2016-04-01 00:00:00
abstract：:Tubular atrophy and interstitial fibrosis, important in progression of renal diseases, including diabetic (D) and cyclosporine-induced (CSA) nephropathy, have been considered irreversible. Normoglycemia for 10 years following pancreas transplantation alone (PTA) reversed D glomerulopathy lesions. This study quantified...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000153
更新日期：2006-03-01 00:00:00
abstract：:Noninvasive molecular tests of urine cells have been developed to monitor the activity of kidney diseases. We evaluate whether measurement of urinary messenger RNA (mRNA) levels of chemokine and growth factor genes could distinguish between diffuse proliferative lupus nephritis (class IV LN) and others and whether it ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000132
更新日期：2006-02-01 00:00:00
abstract：BACKGROUND:The aim of this study was to define cut-off values for serum creatinine as an indicator of several levels of renal impairment. METHODS:To identify the suitable values, receiver operating characteristic curves were constructed based on the data of 984 laboratory assessments of renal function. The glomerular ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00411.x
更新日期：1999-05-01 00:00:00
abstract：:Adoptive immunotherapy in patients with advanced cancer produces significant regression of metastatic disease in selected patients, but it is complicated by severe side effects. Prevention of these complications is still limited because their precise mechanisms remain unknown. For this reason we have investigated rena...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.215
更新日期：1991-08-01 00:00:00
abstract：:In six healthy males on a fixed sodium/potassium (Na/K) intake, we studied the relation between plasma K and urine K and Na excretion after an oral K load. Studies were repeated during fludrocortisone (0.5 mg bid) or spironolactone (50 mg qid), that is, after escape from the Na-retaining and Na-excreting effects of th...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.235
更新日期：1988-11-01 00:00:00
