Abstract：
INTRODUCTION:Diabetes mellitus may be treated with pancreatic islet cell transplantation. The use of xenogenic islet cells may overcome the shortage of human donor organs. Microencapsulation seems to be a promising method for immunoprotection. Since isolation, purification, encapsulation, and transplantation of islet cells are labor-intensive, cryopreservation has emerged as an attractive system for islet banking. In this study sodium cellulose sulfate (NaCS), a novel method for microencapsulation of islet cells, was tested for its capability to protect cells during cryopreservation. METHODS:HIT-T15 cells were microencapsulated in NaCS. Cells were frozen and thawed using three different media containing varying amounts of dimethylsulfoxide (DMSO) and glycerol. Cell viability and cell growth were monitored using 3-(-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide before freezing and 1 week after thawing. RESULTS:NaCS did not show any negative impact on the growth rates of encapsulated HIT-T15 cells compared with nonencapsulated controls. Nonencapsulated cells were adequately cryopreserved by both DMSO- and glycerol-containing freezing media. DMSO was not suitable for cryopreservation of encapsulated HIT-T15 cells, whereas glycerol seemed to produce no considerable cell loss during freezing and thawing. DISCUSSION:Islet banking of cells encapsulated in NaCS was feasible. Microencapsulation did not harm islet cell recovery. As NaCS is less immunogenic and more biocompatible than other materials used for microencapsulation, it may be a promising method for immunoisolation of islet cells to replace the endocrine pancreas in a physiological way.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Stiegler PB,Stadlbauer V,Schaffellner S,Halwachs G,Lackner C,Hauser O,Iberer F,Tscheliessnigg Kdoi
10.1016/j.transproceed.2006.08.188subject
Has Abstractpub_date
2006-11-01 00:00:00pages
3026-30issue
9eissn
0041-1345issn
1873-2623pii
S0041-1345(06)01130-4journal_volume
38pub_type
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journal_title：Transplantation proceedings
pub_type： 杂志文章,评审
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abstract：:For patients with end-stage heart failure, heart transplantation remains one of the most successful therapies with excellent long-term survival rates. However, over the past few decades, there has been a worsening supply/demand mismatch given the rising epidemic of heart failure and the relatively fixed availability o...
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abstract：:Alpha-1 antitrypsin (AAT) is a serine protease inhibitor able to prevent diabetes onset in nonobese diabetic (NOD) mice and to prolong islet allograft survival in a nonautoimmune murine model. In this study, we explored the effect of chronic administration of human AAT (hAAT) on allogeneic (C57BL/6) islet graft surviv...
journal_title：Transplantation proceedings
pub_type： 杂志文章
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abstract：BACKGROUND:A severe shortage in donor organs is the major driver for organ transplantation-related crimes. The Declaration of Istanbul 2008 (DOI) was created to stop such crimes. We investigated the impact of DOI on Internet reporting of transplantation-related crimes. METHODS:We conducted Google Advanced Searches to ...
journal_title：Transplantation proceedings
pub_type： 杂志文章,meta分析
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journal_title：Transplantation proceedings
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.04.013
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.08.140
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.01.029
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2012.05.018
更新日期：2012-07-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.01.135
更新日期：2011-05-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.04.099
更新日期：2019-11-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.07.063
更新日期：2007-09-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.06.008
更新日期：2007-09-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.03.132
更新日期：2008-06-01 00:00:00
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journal_title：Transplantation proceedings
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doi：10.1016/j.transproceed.2017.09.060
更新日期：2018-03-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.12.020
更新日期：2011-01-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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journal_title：Transplantation proceedings
pub_type： 杂志文章
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.08.023
更新日期：2007-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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journal_title：Transplantation proceedings
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更新日期：2016-11-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.08.012
更新日期：2004-09-01 00:00:00
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pub_type： 杂志文章,多中心研究
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更新日期：2016-04-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
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更新日期：2016-09-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
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更新日期：2005-09-01 00:00:00
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journal_title：Transplantation proceedings
pub_type： 杂志文章
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更新日期：2008-09-01 00:00:00
