Mutagenesis of the herpesvirus saimiri terminal repeat region reveals important elements for virus production.

abstract：UNLABELLED:Semen enhances HIV infection in vitro, but how long it retains this activity has not been carefully examined. Immediately postejaculation, semen exists as a semisolid coagulum, which then converts to a more liquid form in a process termed liquefaction. We demonstrate that early during liquefaction, semen exh...

journal_title：Journal of virology

authors： Roan NR,Liu H,Usmani SM,Neidleman J,Müller JA,Avila-Herrera A,Gawanbacht A,Zirafi O,Chu S,Dong M,Kumar ST,Smith JF,Pollard KS,Fändrich M,Kirchhoff F,Münch J,Witkowska HE,Greene WC

更新日期：2014-07-01 00:00:00

abstract：UNLABELLED:The genome of nonsegmented negative-strand RNA viruses is tightly embedded within a nucleocapsid made of a nucleoprotein (N) homopolymer. To ensure processive RNA synthesis, the viral polymerase L in complex with its cofactor phosphoprotein (P) binds the nucleocapsid that constitutes the functional template....

journal_title：Journal of virology

authors： Brunel J,Chopy D,Dosnon M,Bloyet LM,Devaux P,Urzua E,Cattaneo R,Longhi S,Gerlier D

更新日期：2014-09-01 00:00:00

abstract：:Mutations introduced into the capsid gene of duck hepatitis B virus (DHBV) were tested for their effects on viral DNA synthesis and assembly of enveloped viruses. Four classes of mutant phenotypes were observed among a series of deletions of covering the 3' end of the capsid open reading frame. Class I mutant capsids ...

journal_title：Journal of virology

authors： Yu M,Summers J

更新日期：1991-05-01 00:00:00

abstract：:Noroviruses are the causative agents of the majority of viral gastroenteritis outbreaks in humans. During the past 15 years, noroviruses of genotype GGII.4 have caused four epidemic seasons of viral gastroenteritis, during which four novel variants (termed epidemic variants) emerged and displaced the resident viruses....

journal_title：Journal of virology

authors： Siebenga JJ,Vennema H,Renckens B,de Bruin E,van der Veer B,Siezen RJ,Koopmans M

更新日期：2007-09-01 00:00:00

abstract：:After uptake into target cells, many nonenveloped viruses undergo conformational changes in the low-pH environment of the endocytic compartment. This results in exposure of amphipathic viral peptides and/or hydrophobic protein domains that are inserted into and either disrupt or perforate the vesicular membranes. The ...

journal_title：Journal of virology

authors： Bilek G,Matscheko NM,Pickl-Herk A,Weiss VU,Subirats X,Kenndler E,Blaas D

更新日期：2011-08-01 00:00:00

abstract：:A virus recovered from the saliva of a child with chronic active Epstein-Barr virus (EBV) infection for 8 years was shown to induce EBV early antigen (EBV-EA) in Raji cells and to be expressed into EBV-EA in fresh EBV-negative peripheral blood leukocytes. However, it did not replicate its DNA. Oropharyngeal epithelial...

journal_title：Journal of virology

authors： Alfieri C,Joncas JH

更新日期：1987-10-01 00:00:00

abstract：:Klebsiella pneumoniae is a member of the family Enterobacteriaceae, opportunistic pathogens that are among the eight most prevalent infectious agents in hospitals. The emergence of multidrug-resistant strains of K. pneumoniae has became a public health problem globally. To develop an effective antimicrobial agent, we ...

journal_title：Journal of virology

authors： Cui Z,Shen W,Wang Z,Zhang H,Me R,Wang Y,Zeng L,Zhu Y,Qin J,He P,Guo X

更新日期：2012-12-01 00:00:00

abstract：:The replication of the single-stranded DNA (ssDNA) of parvovirus LuIII was studied in synchronized HeLa cells. After infection of the cells in early S phase, synthesis of a replicative form (RF) DNA became detectable as early as 9 h postinfection, i.e., after display of the cellular helper function(s) indispensable fo...

journal_title：Journal of virology

authors： Siegl G,Gautschi M

更新日期：1976-03-01 00:00:00

abstract：:An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1)...

journal_title：Journal of virology

authors： De Leys R,Vanderborght B,Vanden Haesevelde M,Heyndrickx L,van Geel A,Wauters C,Bernaerts R,Saman E,Nijs P,Willems B

更新日期：1990-03-01 00:00:00

abstract：:Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen of dogs, causing high mortality in unvaccinated dogs. After emerging, CPV-2 spread rapidly worldwide. However, there is now some evidence to suggest that international transmission appears to be more restricted. In order to investigate the transmission and ...

journal_title：Journal of virology

authors： Clegg SR,Coyne KP,Parker J,Dawson S,Godsall SA,Pinchbeck G,Cripps PJ,Gaskell RM,Radford AD

更新日期：2011-08-01 00:00:00

abstract：:We are using site-directed mutagenesis of single viral genes to identify and analyze the genetic determinants of human and simian immunodeficiency virus pathogenicity. In a first approach, we have constructed a series of simian immunodeficiency virus SIVmac nef mutants by partial deletion and insertions in the nef gen...

journal_title：Journal of virology

authors： Binninger D,Ennen J,Bonn D,Norley SG,Kurth R

更新日期：1991-10-01 00:00:00

abstract：:Mononuclear phagocytes (MP) and T lymphocytes play a pivotal role in the host immune response to human immunodeficiency virus type 1 (HIV-1) infection. Regulation of such immune responses can be mediated, in part, through the interaction of the T-lymphocyte-expressed molecule CD40 ligand (CD40L) with its receptor on M...

journal_title：Journal of virology

authors： Cotter RL,Zheng J,Che M,Niemann D,Liu Y,He J,Thomas E,Gendelman HE

更新日期：2001-05-01 00:00:00

abstract：:The immunosuppressant drug rapamycin blocks progression of the cell cycle at G1 in mammalian cells and yeast. We recently showed that rapamycin inhibits both in vitro and in vivo cap-dependent, but not cap-independent, translation. This inhibition is causally related to reduced phosphorylation and consequent activatio...

journal_title：Journal of virology

authors： Beretta L,Svitkin YV,Sonenberg N

更新日期：1996-12-01 00:00:00

abstract：:The mechanism of regulation for the ribozyme activity of hepatitis delta virus (HDV) RNA in infected cells is unknown. Previously, we developed a direct assay capable of detecting the ribozyme activity of HDV dimer or trimer RNAs in vivo (K.-S. Jeng, A. Daniel, and M. M. C. Lai, J. Virol, 70:2403-2410, 1996). In this ...

journal_title：Journal of virology

authors： Jeng KS,Su PY,Lai MM

更新日期：1996-07-01 00:00:00

abstract：:The attachment of lymphocytic choriomeningitis virus (LCMV) to murine and primate cell lines was quantitated by a fluorescence-activated cell sorter assay in which binding of biotinylated virus was detected with streptavidin-fluorescein isothiocyanate. Cell lines that were readily infected by LCMV (e.g., MC57, Rin, BH...

journal_title：Journal of virology

authors： Borrow P,Oldstone MB

更新日期：1992-12-01 00:00:00

abstract：:Influenza A viruses (IAV) are lytic viruses that have recently been found to activate necroptosis in many of the cell types they infect. Necroptotic cell death is potently immunogenic and limits IAV spread by directly eliminating infected cells and by mobilizing both innate and adaptive immune responses. The benefits ...

journal_title：Journal of virology

authors： Balachandran S,Rall GF

更新日期：2020-04-16 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) infection causes the active degradation of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), and this process is reliant on the expression of the HSV-1 immediate-early protein Vmw110. In this study we investigated in more detail the mechanism by which the degradation...

journal_title：Journal of virology

authors： Parkinson J,Lees-Miller SP,Everett RD

更新日期：1999-01-01 00:00:00

abstract：:JC polyomavirus (JCV) infection is highly prevalent and usually kept in a persistent state without clinical signs and symptoms. It is only during immunocompromise and especially impaired CD4(+) T cell function in the brain, as seen in AIDS patients or natalizumab-treated multiple sclerosis patients, that JCV may cause...

journal_title：Journal of virology

authors： Jelcic I,Aly L,Binder TM,Jelcic I,Bofill-Mas S,Planas R,Demina V,Eiermann TH,Weber T,Girones R,Sospedra M,Martin R

更新日期：2013-03-01 00:00:00

abstract：:We have previously demonstrated that open reading frame (ORF) 50 and ORF 57 encode transcriptional regulating genes in herpesvirus saimiri. ORF 50, a homolog of Epstein-Barr virus R protein, is a sequence-specific transactivator, whereas ORF 57 acts posttranscriptionally. In this report, we demonstrate that the ORF 57...

journal_title：Journal of virology

authors： Whitehouse A,Cooper M,Hall KT,Meredith DM

更新日期：1998-03-01 00:00:00

abstract：:Although adeno-associated virus type 2 (AAV) has gained attention as a potentially useful vector for human gene therapy, the transduction efficiencies of AAV vectors vary greatly in different cells and tissues in vitro and in vivo. We have documented that a cellular tyrosine phosphoprotein, designated the single-stran...

journal_title：Journal of virology

authors： Qing K,Hansen J,Weigel-Kelley KA,Tan M,Zhou S,Srivastava A

更新日期：2001-10-01 00:00:00

abstract：:Grasshoppers, Melanoplus sanguinipes (F.), infected with the grasshopper inclusion body virus (GIBV) showed a general torpor, took longer to develop, and had abnormally high rates of mortality. Infection was found only in the fat body, and developing viruses and inclusion bodies were observed in the nuclei and cytopla...

journal_title：Journal of virology

authors： Henry JE,Nelson BP,Jutila JW

更新日期：1969-06-01 00:00:00

abstract：:The Cas-Br-E murine leukemia virus is a nondefective retrovirus that induces non-T-, non-B-cell lymphomas in susceptible NIH/Swiss mice. By using a DNA probe derived from Cas-Br-E provirus-flanking sequences, we identified a DNA region, originally called Sic-1, rearranged in 16 of 24 tumors analyzed (67%). All proviru...

journal_title：Journal of virology

authors： Bergeron D,Poliquin L,Kozak CA,Rassart E

更新日期：1991-01-01 00:00:00

abstract：:Although highly active antiretroviral therapy (HAART) for human immunodeficiency virus type 1 (HIV-1) infection can reduce levels of HIV-1 RNA in plasma to below the limit of detection, replication-competent forms of the virus persist in all infected individuals. One form of persistence involves a stable reservoir of ...

journal_title：Journal of virology

authors： Ruff CT,Ray SC,Kwon P,Zinn R,Pendleton A,Hutton N,Ashworth R,Gange S,Quinn TC,Siliciano RF,Persaud D

更新日期：2002-09-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) is a recently described arterivirus responsible for disease in swine worldwide. Comparative sequence analysis of 3'-terminal structural genes of the single-stranded RNA viral genome revealed the presence of two genotypic classes of PRRSV, represented by the p...

journal_title：Journal of virology

authors： Nelsen CJ,Murtaugh MP,Faaberg KS

更新日期：1999-01-01 00:00:00

abstract：:The matrix (M) protein of vesicular stomatitis virus (VSV) is a potent inhibitor in vivo of transcription by all three host RNA polymerases (RNAP). In the case of host RNA polymerase II (RNAPII), the inhibition is due to lack of activity of the TATA-binding protein (TBP), which is a subunit of the basal transcription ...

journal_title：Journal of virology

authors： Yuan H,Puckett S,Lyles DS

更新日期：2001-05-01 00:00:00

abstract：:A molecular clone of mouse-neuroadapted yellow fever 17D virus (SPYF-MN) was used to identify critical determinants of viral neuroinvasiveness in a SCID mouse model. Virus derived from this clone differs from nonneuroinvasive YF5.2iv virus at 29 nucleotide positions, encoding 13 predicted amino acid substitutions and ...

journal_title：Journal of virology

authors： Nickells M,Chambers TJ

更新日期：2003-11-01 00:00:00

abstract：:The gene encoding the precursor polypeptide of the Marek's disease herpesvirus (MDHV) secretory glycoprotein gp57-65 (formerly identified as A antigen) has been sequenced. Previous results had localized the gene to a 4.6-kilobase (kb) segment of the BamHI B fragment in the unique long region of the MDHV genome. S1 nuc...

journal_title：Journal of virology

authors： Coussens PM,Velicer LF

更新日期：1988-07-01 00:00:00

abstract：:Rotavirus is the leading global cause of diarrheal mortality for unvaccinated children under 5 years of age. The outer capsid of rotavirus virions consists of VP7 and VP4 proteins, which determine viral G and P types, respectively, and are primary targets of neutralizing antibodies. Successful vaccination depends upon...

journal_title：Journal of virology

authors： Ogden KM,Tan Y,Akopov A,Stewart LS,McHenry R,Fonnesbeck CJ,Piya B,Carter MH,Fedorova NB,Halpin RA,Shilts MH,Edwards KM,Payne DC,Esona MD,Mijatovic-Rustempasic S,Chappell JD,Patton JT,Halasa NB,Das SR

更新日期：2018-12-10 00:00:00

abstract：:Adenovirus type 12 (Huie) inoculated into cultures of primary whole rat embryo produced foci of morphologically altered cells. The number and identification of these transformed areas was dependent upon the calcium concentration of the medium; more foci appeared in 0.1 mm than in 1.8 mm calcium. Cell lines derived fro...

journal_title：Journal of virology

authors： Freeman AE,Black PH,Wolford R,Huebner RJ

更新日期：1967-04-01 00:00:00

abstract：:To improve the safety of recombinant vaccinia virus vaccines, modified vaccinia virus Ankara (MVA) has been employed, because it has a replication defect in most mammalian cells. Here we apply MVA to human immunodeficiency virus type 1 (HIV-1) vaccine development by incorporating the envelope protein gp160 of HIV-1 pr...

journal_title：Journal of virology

authors： Belyakov IM,Wyatt LS,Ahlers JD,Earl P,Pendleton CD,Kelsall BL,Strober W,Moss B,Berzofsky JA

更新日期：1998-10-01 00:00:00