Abstract:
:Quantum dots (QDs) are fluorescent nanocrystals that are emerging as fine alternatives to the conventional organic dyes. They have several advantages including greater photostability and a wider range of excitation-emission wavelengths. By using mortalin staining as a model, we initially demonstrated that the QDs are more stable and provide better resolution in protein imaging in fixed cells. With the help of an internalizing antibody, we generated internalizing QD (i-QD) and demonstrated its inertness to cell replication, structure, and viability. Based on the superior resolution, stability and inertness, we propose the use of QD staining of mortalin as a cell-based visual assay to screen for senescence-inducing drugs, proteins, and siRNAs.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Kaul Z,Yaguchi T,Chiura HX,Kaul SC,Wadhwa Rdoi
10.1196/annals.1395.040subject
Has Abstractpub_date
2007-04-01 00:00:00pages
368-72eissn
0077-8923issn
1749-6632pii
1100/1/368journal_volume
1100pub_type
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